Simc1 (NM_176987) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213490
Simc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik). Note: ORF is codon optimized
  $1,274.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Simc1
Synonyms 4732471D19Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_176987
ORF Size 4062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176987.4, NP_795961.3
RefSeq Size 4811 bp
RefSeq ORF 4065 bp
Locus ID 319719
UniProt ID E9Q6E9
Cytogenetics 13 B1
MW 146.6 kDa
Summary Inhibits the protease activity of CAPN3.UniProtKB/Swiss-Prot Function
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"NM_176987" in other vectors (2)
SKU Description Size Price
MC224336 Simc1 (untagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik), (10ug) 10 ug
$1,275.00
MG213490 Simc1 (tGFP-tagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik), (10ug). Note: ORF is codon optimized 10 ug
$1,474.00

Plasmid Map for MR213490

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Cloning scheme for MR213490

Circular map for MR213490

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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