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Simc1 (NM_176987) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG213490
Simc1 (tGFP-tagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik), (10ug). Note: ORF is codon optimized
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ORF Plasmid:
tGFP DDK
  $1,474.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Simc1
Synonyms 4732471D19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG213490 representing NM_176987, codon optimized.
Due to the complexity of NM_176987, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGATTTCATCGTCATCTCTGACGACTCTGGTTCTGAGAGTAGCGCTGGGACCCGGTCTGGCCGAG
CTAGGAGGCTTAGGCGGGCTTTGAGTAGGACACCCGGAGCATTGCCGCGGCGAACCGTAGACTTTATTGA
CCTGACCCGCGAGAATCGCGCCAGAACAAAGGACCGCAACGGGCTCTGTGTGATCGATCTGACTCGGAAT
GAGGAAGAAAATCGACCTATAGCGACTCTTGATCTCACTCTGGAGCCTGTCGCTAGCAGTCAGAAAGAGC
CCACATCTCTCCAGACCTGCACTAGCTTGTCCGGAAAAGAGGTCATGGAAGCTCACGAAGATCGCGGATC
TCAACCCGCTGCCCAGAGAATTATCAACAATGATCCGGTGGACCTGGATCTGTTGGAGGAGAACCTGTTT
GAGGGGAGCAGGCCCCCAACAAGCATCAGTCAGGATTCTGTATACCCTCCAGAGCCTAACTGCAGCTCTA
TTACCTATAAAGGCGACCTGAGCTTCCTGACCTCCCTCCAGCTTTCTTCCGATGTGTCTTCATTCTCTTC
CACCTCAAACCAACGCGCCAGCCTGCCTTGCCCACAGCAGGACGTTCCATGCCAGTCACAGGGCTTGCTG
TGTCCTCTTCAGGCTCTGTCTTGTCCCCAAACAGCTTCCCCCTGCCCGCCTCGAGCCAGTTCATGTCCAC
CTCAGGCCCTGTCTTGTCCACCCCAGGCCTTGTCCTGTCCATCCCAAACACTTCAGTGTCAACTGCAGGC
ACTGCCGCAGCCACCTCAAGAGGTTCCATGCTCTACACAAAACGTCCCGTGTCCCCAACAGAACATGCCG
TCAACTCCACAGGGGCTCTCTTGGCATCCACGGCACACACTGTACCCCTATCAAGATACACTGGGCCTCC
CCCAGGACGTTCCTGGGCGGCCACAGAACATGAGCTACCCCCAGGACGTGACCCAACTCCAGGACATGCC
CTGGAGCCTCCACGATATGCCCCTGTCTCTGCAGGACGTACTGCAGTCTCTGCAGGATGTGCCACCTCTG
CTGGGAGACGTTCCTCCCTCTCCCGAAGTTATGCAGCTCCCTGGTTACGTTACCCAGACCAGCAGAGACG
TGATCCAGAGTCCTGCAGGTGTCACTCAGAGCCTGGGGTCAATGATGCAGTCCCCCGGCTCTGTGACCCA
AAGCTTGAGAAGCGTCATGCAGAGCTCTGGCAGCGTGACTCAGTCTCTGCGGTCTGTCATGCAAAGCAGC
GGCTCAGTAACACAGTCCCTGCGATCTGTCATGCAGAGTTCTGGAAGTGTAATGCAGTCCCCTGGTGGAG
TCACACAGAGTCTGCGCAGTGTTACACAGTCTCCGGGCGGCGTGATGCAGAGCCCCGGGGGAGTCATGCA
ATCCCCTCGAGATGTGATGCAATCTCCTCGAGACGTCATGCAGTCTCCTCGAGACGTGATGCAGTCACCC
AGAGGGGTAACTCAAAGCCTGGGATCCATGATGCAATCCCCTGGCGGCGTGATGCAGTCCCTCAGGAGCG
TTATGCAGTCCTCCGGGGGCGTGACACAGTCTCTGAGAAGCGTGATGCAAAGCCCGGGAGGAGTCATGCA
GTCACCTGGAGGAGTTACACAGAGCCCGCGGGGCATGATTAAGTCCCCTGGAATGATGCTGAGCCCTGGG
GATGTAATTCAATCCCTGAACTCAGTGCCTCAGTCTTCTAGGGACCGGATGCAGTCTGCGGGCCACGTGC
CCTCTGCAAGTGGCGACGCAATACAGAGCCCCGGCGGGATGTCTCCCACCTCTCGCGACAGGATGCAGTC
CCCAGGTGGAGTATCCCTGGCAACTGAAGACAGTATCCAGCTCCCTGGCGGTGTGCCACTGTCCTCAGAC
GTCATCCAATCCCAGGGGGGAGTGCCTCTGTCATCCAAAGACAGGATGCAGAGTCCAGGTGGAGTTCCTC
CCAGCTCTGGGGATATGATTCAGTCACAGGGTGGTGTTCCCCAGTCCCTTGGAGACGCAATCCAATCCGC
TGGCGGAGTGCCTCAAAGCAGCGGCGATGCTATCCAATCTCCCGGCGGTGTGAGCCTGGCCACCGGGGAT
AGCATCCAGCTCCCTGGGGGAGTGCCACTCAGTTCTGGGGACGTCATCCAGAGTCAGGGCGGAGTCCCCA
GGTCTTCTCGAGACAGAATGCAGTCTCCGGGAGGGGTGCCTCCCTCTAGTGGAGGGGTTATCCAGTCACC
CGGAGGCGTCTCACCAGCTTCAGGAGATGCGATCCAGTCCCCTGGATCTGTGCTGCCTGCAAGCGGAGAT
GCAATCCAGAGCGCTGGCGGCGTGCTGCTTGCGAGCGGAGACGCCATCCAGTCACCTCGGAGCGTGCCCC
AGAGCCCCTCAGGGACCCTGCGCTCACCGGGTAACATGTCCGAGTCCCTTGGCGACACCCCAAACTTGTC
TGGGGACGTCTCAAACTCCCCACAGGCACTGTTGGATCTGGCACGAGATCGCCCAAAGTCTTCCCCTAAC
GACGTTCAGAATAGAGACACACCAATGGACATTAGCGCTTCATCTTCCTCTAGTTGTAGTGCCAATCCCC
AGTCTAGGCAGAGCGAGTTTAAACTGGACAAGGTGCCATGGCTTACCATCACTGACTCAAGTGCACGAAG
GGAGAAATCTCTGCCCCAGCTCGCCAACCCAGGTTCCGCCCAGATCCAAGGCCAGATTCCACAAGTGGGT
GTTTATAATCGCCCATGTCTTCACCGGCTCAAGTATTTCCTTCGACCACCTGTGCATCACCTCTTCTTTC
AGACCCTCATCCCTGACAAGGATACCAGGGAAAGTAAAGGGCAGAAGCTGGAACCAATCCCTCACCGGAG
ACTCCGAATGGTCACGAACACAATCGAGGAAAACTTCCCTCTCGGCACAGTGCAATTCCTCATGGACTTT
GTTAGTCCACAACACTATCCCCCCAGAGAGATCGTCGCGCACATCGTTCAGAAGATCCTTCTTAGCGGAA
GCGAAACTGTGGACGTGCTGAAGGAAGCCTACATGCTCCTCATGAAAATCCAACAACTCCATCCCGCCAA
CGCCAAAACCGTAGAGTGGGACTGGAAGCTCCTGACTTACGTCATGGAGGAGGAGGGCCAAACCCTGCCT
GGGAGGGTGCTGTTCCTGCGGTACGTGGTGCAGACTCTTGAAGATGACTTCCAACAGATCCTGCGGAGAC
AGCGGCAGCATCTGCAGCAGTCTATAGCCAACACCGTTCTTTCCTGCGATAAGCAACCTCACAACGTCAG
GGATGTAATTAAATGGCTTGTGAAGGCTGTCACGGAAAATGAACTGACCCCCCCACAGGACGAGACTCAG
ACCAGTCCAAGAACGGGCGTGCTCAAGACATCATCTGATCACCTCTCTCCCCGACCGAATCAGAATAAAA
ACACAAACCAGCTGATAGTTTGCCAGTTGCAAAGGATGTTGTCTATTGCGGTCGAAGTGGATAGGACCCC
AACCTGCTCATCTAACAAGATCGCGGAGATGATGTTTGGATTCGTTCTCGACATCCCAGAACGCTCCCAG
CGCGAAATGTTTTTCACAACCATGGAGTCCCACCTGCTGAGATGCAAAGTGTTGGAGATCATTTTCCTCC
ACTCATGCGAAACTCCTACTAGGCTTCCACTCTCCCTCGCGCAGACACTCTATTTCTTGAACAACTCTAC
TTCTCTTCTGAAGTGTCAGAGCGACAAAACACAGTGGCAGACTTGGGACGAGCTGGTCGAACACCTCCAG
TTCCTCCTGTCCTCTTATCAGCATGTTCTGCGCGAGCACCTGCGATCTTCTGTCATAGACCGCAAGGACC
TCATCATTAAACGCATCAAGCCCAAGCCACAGCAGGGAGACGACATCACCGTGTTGGACGTTGAGAAACA
GATTGAGGCGTTCAGGTCTAGGCTCGTTCACATTCTGGGGGAGCCACTTGTGCCCCAGCTCCAGGATAAG
GTACATCTGCTGAAGCTGCTGCTGTTCTACGCCGCCGACCTCAACCCTGATACCGAACCCGCTTCCGAGC
AC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG213490 representing NM_176987
Red=Cloning site Green=Tags(s)

MEDFIVISDDSGSESSAGTRSGRARRLRRALSRTPGALPRRTVDFIDLTRENRARTKDRNGLCVIDLTRN
EEENRPIATLDLTLEPVASSQKEPTSLQTCTSLSGKEVMEAHEDRGSQPAAQRIINNDPVDLDLLEENLF
EGSRPPTSISQDSVYPPEPNCSSITYKGDLSFLTSLQLSSDVSSFSSTSNQRASLPCPQQDVPCQSQGLL
CPLQALSCPQTASPCPPRASSCPPQALSCPPQALSCPSQTLQCQLQALPQPPQEVPCSTQNVPCPQQNMP
STPQGLSWHPRHTLYPYQDTLGLPQDVPGRPQNMSYPQDVTQLQDMPWSLHDMPLSLQDVLQSLQDVPPL
LGDVPPSPEVMQLPGYVTQTSRDVIQSPAGVTQSLGSMMQSPGSVTQSLRSVMQSSGSVTQSLRSVMQSS
GSVTQSLRSVMQSSGSVMQSPGGVTQSLRSVTQSPGGVMQSPGGVMQSPRDVMQSPRDVMQSPRDVMQSP
RGVTQSLGSMMQSPGGVMQSLRSVMQSSGGVTQSLRSVMQSPGGVMQSPGGVTQSPRGMIKSPGMMLSPG
DVIQSLNSVPQSSRDRMQSAGHVPSASGDAIQSPGGMSPTSRDRMQSPGGVSLATEDSIQLPGGVPLSSD
VIQSQGGVPLSSKDRMQSPGGVPPSSGDMIQSQGGVPQSLGDAIQSAGGVPQSSGDAIQSPGGVSLATGD
SIQLPGGVPLSSGDVIQSQGGVPRSSRDRMQSPGGVPPSSGGVIQSPGGVSPASGDAIQSPGSVLPASGD
AIQSAGGVLLASGDAIQSPRSVPQSPSGTLRSPGNMSESLGDTPNLSGDVSNSPQALLDLARDRPKSSPN
DVQNRDTPMDISASSSSSCSANPQSRQSEFKLDKVPWLTITDSSARREKSLPQLANPGSAQIQGQIPQVG
VYNRPCLHRLKYFLRPPVHHLFFQTLIPDKDTRESKGQKLEPIPHRRLRMVTNTIEENFPLGTVQFLMDF
VSPQHYPPREIVAHIVQKILLSGSETVDVLKEAYMLLMKIQQLHPANAKTVEWDWKLLTYVMEEEGQTLP
GRVLFLRYVVQTLEDDFQQILRRQRQHLQQSIANTVLSCDKQPHNVRDVIKWLVKAVTENELTPPQDETQ
TSPRTGVLKTSSDHLSPRPNQNKNTNQLIVCQLQRMLSIAVEVDRTPTCSSNKIAEMMFGFVLDIPERSQ
REMFFTTMESHLLRCKVLEIIFLHSCETPTRLPLSLAQTLYFLNNSTSLLKCQSDKTQWQTWDELVEHLQ
FLLSSYQHVLREHLRSSVIDRKDLIIKRIKPKPQQGDDITVLDVEKQIEAFRSRLVHILGEPLVPQLQDK
VHLLKLLLFYAADLNPDTEPASEH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_176987
ORF Size 4062 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_176987.4, NP_795961.3
RefSeq Size 4811 bp
RefSeq ORF 4065 bp
Locus ID 319719
UniProt ID E9Q6E9
Cytogenetics 13 B1
Summary Inhibits the protease activity of CAPN3.UniProtKB/Swiss-Prot Function
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"NM_176987" in other vectors (2)
SKU Description Size Price
MC224336 Simc1 (untagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik), (10ug) 10 ug
$1,275.00
MR213490 Simc1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 4732471D19 gene (4732471D19Rik). Note: ORF is codon optimized 10 ug
$1,274.00

Plasmid Map for MG213490

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG213490
Circular map for MG213490

file-alt Citations

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