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Fyb2 (NM_001162980) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213445
1700024P16Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700024P16 gene (1700024P16Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $727.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fyb2
Synonyms 1700024P16Rik; ARAP; C1orf168; Gm620
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213445 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCCGCCCACCCGAGAGAGGGAGGAGCCACAGCCCAGGTGGAGCAGGTGAGGAGCCCCGGCTCC
AGCTCCTCCGGGACAGGCGAAGTGACTGCAAGCTGGCTGGCGGAGGCGAGAGCATTGTTGGCCCCAGGAT
CCAACTCTGGGAAGCTGGCATTGTCTCCCAGTCGCTGCCTAGGAGGACCACAATGGAGGAGGAAGGGATA
AGAAACTTCAAGGAACTTCGAGCCAAATTTCAAAAGTTTAATGCTGCACCTCTTCCAGGGCCAATGAGGT
TTCCAGCTGGCGTGTCTCGAAAGCATGACAGAGGTTCCACACAGCCAGCTCAGGATCTGGCTAATAGGAA
GTGCCTCTCATCCCACCATCATCGAACCCCGTCTCATTCTTCCACTGGAGTGTCCCAGCCTCTTAAAAAC
CAGACATTGAAGTCGGCTCAGGGAGATGAACTTCAGAAGACTTCAAGTTCCTCGGGAACTCCAGAAAAAT
CTACAGTGTGTCCTGAAAGGGATTTCCAGAAAGCTGCCGTGCCATTGGATGTGACTAAATCAAGAGCTAA
GACATCCAACGAGGAGAAAGGAATGACTCTCAGTAGCTTCAGATACAAGCTTTGGAACTGGGAGAAAGTT
TCATCTCAGAACGGTGAAATGTCCCCAGCTCCACTCCTTACCAATGGCGGGATTAAGACCTTCCACTTTG
AAGGGCAGAAAACCATGGGGCTTGCTCAGGACAAACCAGAGAAGAGCCTGAAGGCAACAGGAGCCCAGAC
TCTTCCTCCCCAGAGCCATTCGATGGCCCAGAGAAAATCACCCGTGACCTCCAAAGCCTCCAGTGTATCA
CTTCCTCCACATAGCAGGAAGAGCACAAAAAGCCCTGCCACTGAGGGAAGCCACAGGAGCAGCCAATGCC
AGCCTGTTTATGAGTGTGAACTTGACAGCCTGGTTCCAGAGAAGCCAGAGAGCAGGCACTGCCAGCTTCC
CAAAACAAAACCACTGCCTTCCATTGAGACCCTCGGTCCACCTCCTCCGAAGCCTTCAAAGCCCCCATTC
GTGAACCTCTATGCCTTCCACAAGCTGCCAGCTACTGTCACCAAGACCCCGAAAGAAGAGACTATGAAGG
AGGGTCCCCTGTCTCCAGATAGTGCAGAGTTGGAAGAAGCACATAATTATGACACGACAATTTCGTATCT
GAGACACTCTGGCAACTCCATTAACTTGTGTGCTGAGGGAGAAAGTACTGAAGCCACTTATGAAATTGAG
ATTGAAGAACTCCAAAAGCCTTGGAGGAGTTTTTTCCTTCCAGAACTGAGTCCTAGACCTAAAGAGGAAG
AAAACACCATGGAGGAAAAGGAGTCGTGGGAATCGGAACCTCTGGAGCCAAGGAAGGAGTTACACCCTAG
TCGTCCTCCCAAGGTAGTTGTCTACAAAGAGACTCCAGGAAAAACCCAGATGGCTGGAGTCCATGAGGAC
AGAAGGAGTGTGCCAGCTGGAAACCAGGAAGCCATGACTGACATCATGCAGAACAGGCTCTTCCCCGAGG
ATGTGACGCTGACGAGGCATTCCCAGGATAAGAGTGGGTACGTGGAGGCTTTGGAGGTGACGAAAGAAAC
GCCAAGTCCCAGCACCATTAGGTCAAGCTCCTCTTCAGAGAAGACTTATGATGCCGTGGAGTGCTCCAGA
GAAGACGTACGAAAGTGGGACTTCTCCAGCTCATTCACCTCAGACAGTGAAGAAAATTGTGAAGAAATGT
ATGAAGATATCTACAAAGCAAAGAACGATGATCCTAAAACAGAAGTAGCTGGGAGGACAGCACTGAGAAA
ACTGCAGCAGATCTTCAGGAAAGAAAACGTCGTGTTCAGAATGAAGAAAACCAAGTCAAAAGAAATTGTC
AGCAATGGATTTTCCGTGTCACTGCCTGACTTGGGACCTAGGTCTCAGGATGATTCTCAGGATGGTATCA
TCTACGATGATGTGGATACACGTGAGAAAGAATCAAACGATGAGGATAAAGTAAAAACTTGGAAGACTAA
GTTTTTGATACCAAAAGGAAAAAAGTGGGGGAAAGGAAGCCAGGGATCCAAAAGTTTTTCTCCCAGACAT
TTCTTCAGAACTAAGAAGCAAAAATTAGAAAAGAATAGAATGGAAAAAGAAGAAAAACTCTTCAGAGAAC
GATTTCAGTATGGTAAAGAAATCCTGGTCATTAACAGAGCTGTAGCGTGTGCCAGTAATTCACGAAATGG
AATGTTTGATCTGCCAATAATTCCTGGCGAACAGCTGGAAGTCATCGACACCACTGAGCAAAACCTAGTG
ATCTGTCGCAATTCCAAAGGCAAATATGGATATGTGCTCGTTGAACATCTAGATTTCAAGCATCAGGGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213445 protein sequence
Red=Cloning site Green=Tags(s)

MEARPPERGRSHSPGGAGEEPRLQLLRDRRSDCKLAGGGESIVGPRIQLWEAGIVSQSLPRRTTMEEEGI
RNFKELRAKFQKFNAAPLPGPMRFPAGVSRKHDRGSTQPAQDLANRKCLSSHHHRTPSHSSTGVSQPLKN
QTLKSAQGDELQKTSSSSGTPEKSTVCPERDFQKAAVPLDVTKSRAKTSNEEKGMTLSSFRYKLWNWEKV
SSQNGEMSPAPLLTNGGIKTFHFEGQKTMGLAQDKPEKSLKATGAQTLPPQSHSMAQRKSPVTSKASSVS
LPPHSRKSTKSPATEGSHRSSQCQPVYECELDSLVPEKPESRHCQLPKTKPLPSIETLGPPPPKPSKPPF
VNLYAFHKLPATVTKTPKEETMKEGPLSPDSAELEEAHNYDTTISYLRHSGNSINLCAEGESTEATYEIE
IEELQKPWRSFFLPELSPRPKEEENTMEEKESWESEPLEPRKELHPSRPPKVVVYKETPGKTQMAGVHED
RRSVPAGNQEAMTDIMQNRLFPEDVTLTRHSQDKSGYVEALEVTKETPSPSTIRSSSSSEKTYDAVECSR
EDVRKWDFSSSFTSDSEENCEEMYEDIYKAKNDDPKTEVAGRTALRKLQQIFRKENVVFRMKKTKSKEIV
SNGFSVSLPDLGPRSQDDSQDGIIYDDVDTREKESNDEDKVKTWKTKFLIPKGKKWGKGSQGSKSFSPRH
FFRTKKQKLEKNRMEKEEKLFRERFQYGKEILVINRAVACASNSRNGMFDLPIIPGEQLEVIDTTEQNLV
ICRNSKGKYGYVLVEHLDFKHQG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001162980
ORF Size 2379 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001162980.1, NP_001156452.1
RefSeq Size 3136 bp
RefSeq ORF 2382 bp
Locus ID 242594
Cytogenetics 4 C6
MW 89.2 kDa
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Documents
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"NM_001162980" in other vectors (4)
SKU Description Size Price
MC221664 1700024P16Rik (untagged) - Mouse RIKEN cDNA 1700024P16 gene (1700024P16Rik), (10ug) 10 ug
$800.00
MG213445 1700024P16Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700024P16 gene (1700024P16Rik), (10ug) 10 ug
$927.00
MR213445L3 Lenti ORF clone of 1700024P16Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700024P16 gene (1700024P16Rik) 10 ug
$1,027.00
MR213445L4 Lenti ORF clone of 1700024P16Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700024P16 gene (1700024P16Rik) 10 ug
$1,027.00

Plasmid Map for MR213445

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR213445
Circular map for MR213445

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