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Ifit1bl2 (NM_053217) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR213255
2010002M12Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010002M12 gene (2010002M12Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ifit1bl2 or NM_053217"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ifit1bl2
Synonyms 2010002M12Rik; AW412491; Ifit1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213255 representing NM_053217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCAACTTCTGAGTCCCAGCAACATGAGTGAAGAATCTCATAAAAGTCATATACATGACAGCCTGG
ATGAGCTGAGATGCCACTTTACCTGGGAGCTGGACATAAAAGATAAGCACATCCATGACTTAGAAATCAA
AATCTCAGAGACTGAGTTCCGTGATCCCATCTACAGCATCGGGATGCACAACCTCCTGGCCTACGTGAGG
CACCTGAAAGGCCAGCAGGACGAAGCCCTGCAGAGCTTGAAAGAAGCTGAAGCCTTGATCCAGAGCGAGC
AGCTGAGCAAGAGAAGCCTGGCGACCTGGGGCAACTGTGCCTGGCTGCATTACCACAGGGGCAGCTTGGC
AGAAGCCCAGGTCTACCTGGACAAGGTGGAGAAGGTGTGCAAGGAATTTTCAAGTCCCTTCCGCTACAGG
CTGGAGTGTGCTGAGATGGACTGTGAGGAAGGCTGGGCCTTGCGGAAGTGTGGCTCACAAAATTATACAC
GAGCCATGGCCTGCTTTGAAAGGGCTCTGAAAGTGGAGCCAGAAAACCCTGAGTACAACGCTGGCTATGC
AGATGTAGCCTATCACCTGGATTACTATGATGGTAATTCTCTACAACCCTTAAAGAAGGCTGTCAGTGTA
AAACCAGAAGATCCATACCTTAAAGTTCTCCTTGCCCTAAAGCTGCAGGATTTAAGAAAAACAGATGAAG
CAGAAAAACACATTAAAGAAGCTACGCTCACAATATCCTCCCAGAACAATATCTTTGGATATGTAGCCAA
ATTTTACCGAAGGAAAGGTTGTGTGGAGGAAGCTCTCGGGTTCCTTAAAAAAGCCTTGGAGACAAAACCT
TCCTCTCCTTACTTGCATTTCCAGATTGGCCTTTGCCATAAAACACAATTTTTTCAAATGAAAAAAGCTA
CAAGCAGGGAGAATAGAAAAAGAGCAGACCAATCATGCCATTTGGCTATATGTCATTTTAAAAAGACTTT
AGAGCTGAAACCCACATATGACAGAGCATATATTGATTTGGCTGAGGTGTACGCAAAAAATCATCAACAA
AAAGAGGCAGAGGACAATTTTCAGGAAGTGTTGAGCATGAGTAACTTGGGAGATTATATGCAGCAAGAAA
TTCATTTCCGGTATGGCAACTTCCAACAATACTATAAGAAATCAGAAGAGGCAGCCATCACACATTACTT
AAAAGGCCTGAAAATAGAGGTAACTTCTCACTACAGGGATAAACTTCTCAAGGCTTTGGAGGAACTGGCT
GAAGGACGCAAGGAGGATCATGTCCTGGAAAGCTTGAGCCTCCTTGGGCTCGTCTGCAGACTGAGAGGGG
ACACAAGTGAAGCCATGTCATGTTATGAGAAGGCCCTGAGGCTCACAGGAGCAGTGAACCCTGAGTTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR213255 representing NM_053217
Red=Cloning site Green=Tags(s)

MEQLLSPSNMSEESHKSHIHDSLDELRCHFTWELDIKDKHIHDLEIKISETEFRDPIYSIGMHNLLAYVR
HLKGQQDEALQSLKEAEALIQSEQLSKRSLATWGNCAWLHYHRGSLAEAQVYLDKVEKVCKEFSSPFRYR
LECAEMDCEEGWALRKCGSQNYTRAMACFERALKVEPENPEYNAGYADVAYHLDYYDGNSLQPLKKAVSV
KPEDPYLKVLLALKLQDLRKTDEAEKHIKEATLTISSQNNIFGYVAKFYRRKGCVEEALGFLKKALETKP
SSPYLHFQIGLCHKTQFFQMKKATSRENRKRADQSCHLAICHFKKTLELKPTYDRAYIDLAEVYAKNHQQ
KEAEDNFQEVLSMSNLGDYMQQEIHFRYGNFQQYYKKSEEAAITHYLKGLKIEVTSHYRDKLLKALEELA
EGRKEDHVLESLSLLGLVCRLRGDTSEAMSCYEKALRLTGAVNPEF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053217
ORF Size 1398 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053217.3
RefSeq Size 3365 bp
RefSeq ORF 1401 bp
Locus ID 112419
Cytogenetics 19 C1
MW 54.5 kDa
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"NM_053217" in other vectors (4)
SKU Description Size Price
MC216356 2010002M12Rik (untagged) - Mouse RIKEN cDNA 2010002M12 gene (2010002M12Rik), (10ug) 10 ug
$503.00
MG213255 2010002M12Rik (tGFP-tagged) - Mouse RIKEN cDNA 2010002M12 gene (2010002M12Rik), (10ug) 10 ug
$657.00
MR213255L3 Lenti ORF clone of 2010002M12Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 2010002M12 gene (2010002M12Rik) 10 ug
$757.00
MR213255L4 Lenti ORF clone of 2010002M12Rik (mGFP-tagged) - Mouse RIKEN cDNA 2010002M12 gene (2010002M12Rik) 10 ug
$757.00

Plasmid Map for MR213255

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR213255
Circular map for MR213255

file-alt Citations

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