Mtch1 (NM_019880) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR213020
Mtch1 (Myc-DDK-tagged) - Mouse mitochondrial carrier homolog 1 (C. elegans) (Mtch1), nuclear gene encoding mitochondrial protein
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mtch1
Synonyms 2310034O17Rik; AI255158; AU018396; C77849; PSAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR213020 representing NM_019880
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGCTTCGGACCCGGAAGTGGCGCCTTGGGCTCCCGGCGGCGCCGCGGGGATGGCGGGAGCCGGAG
CTGGTGCAGGAGCTCGGGGCGGCGCGCCGGCCGGAGTCGAGGCCCGCGCTCGGGACCCGCCACCCGCGCA
CCGCGCGCACCCTCGCCATCCTCGGCCCGCGGCTCAGCCGTCGGCGCGCAGGATGGACGGCGGCCCGGGC
GCCCCGGGCTCCGGGGACAACGCCCCGACCACCGAGGCGCTGTTCGTGGCGCTGGGCGCGGGCGTGACGG
CTCTCAGTCACCCGCTGCTCTACGTGAAGCTGCTGATCCAGGTGGGTCATGAGCCGATGCCCCCCACCCT
TGGGACCAATGTGCTGGGGAGGAAGGTCCTCTACCTGCCGAGCTTCTTCACCTATGCCAAGTACATTGTG
CAGGTGGATGGGAAGATAGGGCTCTTCCGGGGCCTGAGCCCCCGCCTTATGTCCAACGCCTTGTCCACTG
TGACCCGCGGCAGCATGAAGAAGGTTTTCCCTCCAGATGAGATGGAGCAGGTTTCCAACAAGGACGACAT
GAAGACCTCACTCAAGAAAGTTGTGAAGGAGACATCGTATGAGATGATGATGCAGTGTGTATCGCGAATG
CTGGCCCATCCCTTACACGTGATCTCGATGCGATGCATGGTGCAGTTTGTGGGACGGGAGGCCAAGTACA
GTGGTGTGCTGAGTTCTATTGGGAAGATCTTCAAGGAAGAGGGGCTGCTGGGATTCTTCGTTGGCTTAAT
CCCTCACCTCCTGGGCGATGTGGTTTTCTTGTGGGGCTGTAACCTGCTGGCCCACTTCATCAATGCCTAC
TTGGTGGACGACAGCGTGAGTGACACCCCAGGGGGGCTGGGAAACGACCAGAATCCAGGTTCCCAGTTTA
GCCAGGCCCTGGCCATCCGGAGCTACACCAAGTTTGTGATGGGGATTGCAGTGAGCATGCTGACCTACCC
CTTCCTGCTCGTTGGAGATCTCATGGCAGTGAACAACTGTGGGCTGCGGGCTGGACTCCCTCCGTATTCC
CCTGTGTTCAAGTCCTGGATCCACTGCTGGAAGTACCTGAGTGTGCAGGGCCAGCTCTTCCGCGGCTCCA
GCCTGCTTTTCCGCCGGGTGTCATCGGGGTCATGCTTTGCCCTGGAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR213020 representing NM_019880
Red=Cloning site Green=Tags(s)

MGASDPEVAPWAPGGAAGMAGAGAGAGARGGAPAGVEARARDPPPAHRAHPRHPRPAAQPSARRMDGGPG
APGSGDNAPTTEALFVALGAGVTALSHPLLYVKLLIQVGHEPMPPTLGTNVLGRKVLYLPSFFTYAKYIV
QVDGKIGLFRGLSPRLMSNALSTVTRGSMKKVFPPDEMEQVSNKDDMKTSLKKVVKETSYEMMMQCVSRM
LAHPLHVISMRCMVQFVGREAKYSGVLSSIGKIFKEEGLLGFFVGLIPHLLGDVVFLWGCNLLAHFINAY
LVDDSVSDTPGGLGNDQNPGSQFSQALAIRSYTKFVMGIAVSMLTYPFLLVGDLMAVNNCGLRAGLPPYS
PVFKSWIHCWKYLSVQGQLFRGSSLLFRRVSSGSCFALE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019880
ORF Size 1167 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019880.4
RefSeq Size 1918 bp
RefSeq ORF 1170 bp
Locus ID 56462
UniProt ID Q791T5
Cytogenetics 17 A3.3
MW 42 kDa
Summary Potential mitochondrial transporter. May play a role in apoptosis (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019880" in other vectors (4)
SKU Description Size Price
MC210058 Mtch1 (untagged) - Mouse mitochondrial carrier homolog 1 (C. elegans) (Mtch1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG213020 Mtch1 (tGFP-tagged) - Mouse mitochondrial carrier homolog 1 (C. elegans) (Mtch1) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$657.00
MR213020L3 Lenti ORF clone of Mtch1 (Myc-DDK-tagged) - Mouse mitochondrial carrier homolog 1 (C. elegans) (Mtch1), nuclear gene encoding mitochondrial protein 10 ug
$757.00
MR213020L4 Lenti ORF clone of Mtch1 (mGFP-tagged) - Mouse mitochondrial carrier homolog 1 (C. elegans) (Mtch1), nuclear gene encoding mitochondrial protein 10 ug
$757.00

Plasmid Map for MR213020

Download sequence for

Cloning scheme for MR213020

Circular map for MR213020

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