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Olfr197 (NM_146484) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212901
Olfr197 (Myc-DDK-tagged) - Mouse olfactory receptor 197 (Olfr197)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Olfr197 or NM_146484"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr197
Synonyms MOR183-3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212901 representing NM_146484
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAACAGAGAACACAACACTGCTGACAGAATTTGTTCTCACGGGACTCAGTCACCAGCCACAATGGA
AAATCCCCCTGTTCCTGCTGTTCTTGGTCATCTATCTTATCACCATTGTGGGGAACCTTGGTCTGATCAT
TCTCATCTGGAATGACCCTCGCCTTCAAATACCCATGTACCTATTTCTTGGGTGTTTAGCTCTTGTTGAT
ACTTTGTTATCATCCACAGTGACACCAAAGATGCTCCTCACCTTCACAGCTAAGAGCAAACTGATCTCTC
TTTCTGAATGCATGATACAGTTCTTTTCATTTGTGATGTGTGCAACTATGGAATGTTTTCTCTTGGCAGC
AATGGCCTACGATCGCTATGCAGCCATATGCAAACCCTTACTGTACCCAGTGATCATGACAAATAGACTG
TGTGTTCGTCTGTTAGTCTTGACCTTTGTAACTGGAATTCTTCATGCTTCAATTCATGAAGGCATGCTGT
GCCGATTAACCTTCTGTAATTCCAATGTAATACATCACTTTTACTGTGATGTTATGCCACTGTTAAAGAT
TTCCTGTACCGACCCTTCTCTTAATTACCTGATTGTTTTTATTTTCTCTGGCTCAATTCAAGTTTTCACT
ATCTCAACTATTCTAGGCTCTTACACACTTGTTCTCTTTACAATCTTAAAAAAGAAATCTGCCAAAGGCA
TAAAGAAAGCCTTCTCCACCTGTGGAGCTCATCTCCTTTCTGTGTGCTTATATTATGGTTCTCTTCTCTT
CATGTATCTGCTTCCTGCATCCCAAAAAGCACAGGATGAAGACATGATGGACTCTGTATTCTACACTGTC
ATAATCCCTGTGATGAATCCAATCATCTATAGTTTGAGAAACAAGCAAATCAAAGACTCACTGGAAAAAT
TCTTAAAGAGAAATGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212901 representing NM_146484
Red=Cloning site Green=Tags(s)

MGTENTTLLTEFVLTGLSHQPQWKIPLFLLFLVIYLITIVGNLGLIILIWNDPRLQIPMYLFLGCLALVD
TLLSSTVTPKMLLTFTAKSKLISLSECMIQFFSFVMCATMECFLLAAMAYDRYAAICKPLLYPVIMTNRL
CVRLLVLTFVTGILHASIHEGMLCRLTFCNSNVIHHFYCDVMPLLKISCTDPSLNYLIVFIFSGSIQVFT
ISTILGSYTLVLFTILKKKSAKGIKKAFSTCGAHLLSVCLYYGSLLFMYLLPASQKAQDEDMMDSVFYTV
IIPVMNPIIYSLRNKQIKDSLEKFLKRNV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_146484
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_146484.1, NP_666695.1
RefSeq Size 930 bp
RefSeq ORF 930 bp
Locus ID 258477
Cytogenetics 16 C1.3
MW 34.9 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This olfactory receptor gene appears to represent a strain-specific polymorphic pseudogene in mouse, where some strains, including the C57BL/6 reference genome strain, have a non-functional allele, while other strains have a protein-coding allele. provided by RefSeq, Jan 2019
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"NM_146484" in other vectors (4)
SKU Description Size Price
MC213653 Olfr197 (untagged) - Mouse olfactory receptor 197 (Olfr197), (10ug) 10 ug
$330.00
MG212901 Olfr197 (tGFP-tagged) - Mouse olfactory receptor 197 (Olfr197), (10ug) 10 ug
$500.00
MR212901L3 Lenti ORF clone of Olfr197 (Myc-DDK-tagged) - Mouse olfactory receptor 197 (Olfr197) 10 ug
$600.00
MR212901L4 Lenti ORF clone of Olfr197 (mGFP-tagged) - Mouse olfactory receptor 197 (Olfr197) 10 ug
$600.00

Plasmid Map for MR212901

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212901
Circular map for MR212901

file-alt Citations

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