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Them4 (NM_029431) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR212675
Them4 (Myc-DDK-tagged) - Mouse thioesterase superfamily member 4 (Them4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Them4 or NM_029431"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Them4
Synonyms 2700077M13Rik; 4921507I02Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR212675 representing NM_029431
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGAAACTGCGCTATGCGCCTGCGCACTCTTGGGGCCACGCCTGCGAGGCGGCCAGGAGCAGCTA
GGAGGTTATTTTCTTCTGAGAAAGTCATTCGTAAGGACTATGCTCTCCCTAACCCCAGCTGGACTAAGGA
CCTCAGACTCCTTTTTGATCAGTTCATGAAGAAATGTGAAGATGGCTCCTGGAAACGCATGCCTTCACAC
AGACAAAACCCTACTCGAGCTATTCAAGAGTTCCAAACCCTTTTTGTTGACTCGAAGTTTAAGAAAGAAG
AACAAATGTCAAAGGCCCAACAGTTCACCAGAAGTTTCGAGGAAGGGCTAGGCTTTGAGTATGCGATGTT
CTATAATAAAGTTGAGAAGAGGACGGTCTCCTTGTTTCAAGGAGGACTTCACCTGCAAGGAGTACCTGGA
TTTGTTCACGGAGGTGCCATTGCAACCATCATTGATGTCACTACTGGCACGTGTGCAATTTCCGAAGGCG
TTGCCATGACTGCCAACCTCAACATCACCTATAAAAAGCCTATCCCCCTTCTTTCTGTTGTTGTGGTAAA
TAGCCAACTTCAAAAAATTGAAGGAAGGAAACTTTTTGTTTCCTGTACTATTCAAAGTATTGATGAGAAG
ACTTTATACACAGAGGCAACGGCCTTATTTATAAAGCTGGATCCTGAGAAACCTTTGACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR212675 representing NM_029431
Red=Cloning site Green=Tags(s)

MLRNCAMRLRTLGATPARRPGAARRLFSSEKVIRKDYALPNPSWTKDLRLLFDQFMKKCEDGSWKRMPSH
RQNPTRAIQEFQTLFVDSKFKKEEQMSKAQQFTRSFEEGLGFEYAMFYNKVEKRTVSLFQGGLHLQGVPG
FVHGGAIATIIDVTTGTCAISEGVAMTANLNITYKKPIPLLSVVVVNSQLQKIEGRKLFVSCTIQSIDEK
TLYTEATALFIKLDPEKPLT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029431
ORF Size 690 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029431.1, NP_083707.1
RefSeq Size 1730 bp
RefSeq ORF 693 bp
Locus ID 75778
UniProt ID Q3UUI3
Cytogenetics 3 F2.1
MW 26.5 kDa
Summary Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism (By similarity). Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity (PubMed:19421406).UniProtKB/Swiss-Prot Function
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Documents
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"NM_029431" in other vectors (4)
SKU Description Size Price
MC211697 Them4 (untagged) - Mouse thioesterase superfamily member 4 (Them4), (10ug) 10 ug
$330.00
MG212675 Them4 (tGFP-tagged) - Mouse thioesterase superfamily member 4 (Them4), (10ug) 10 ug
$500.00
MR212675L3 Lenti ORF clone of Them4 (Myc-DDK-tagged) - Mouse thioesterase superfamily member 4 (Them4) 10 ug
$600.00
MR212675L4 Lenti ORF clone of Them4 (mGFP-tagged) - Mouse thioesterase superfamily member 4 (Them4) 10 ug
$600.00

Plasmid Map for MR212675

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR212675
Circular map for MR212675

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