Notch1 (NM_008714) Mouse Tagged ORF Clone
$3,133.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Notch1 |
| Synonyms | 9930111A19Rik; lin-12; Mis6; N1; Tan1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008714 |
| ORF Size | 7593 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008714.2, NP_032740.2 |
| RefSeq Size | 9497 bp |
| RefSeq ORF | 7596 bp |
| Locus ID | 18128 |
| UniProt ID | Q01705 |
| Cytogenetics | 2 18.91 cM |
| MW | 271.3 kDa |
| Summary | Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4(+) and CD8(+) cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008714" in other vectors (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC225345 | Notch1 (untagged) - Mouse Notch gene homolog 1 (Drosophila) (Notch1), (10ug) | 10 ug |
$3,343.00
|
|
Citations
NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and through transendocytosis into ligand cells
Geles, Gao, Giannakou et al
Cell Rep Med (2021) 2 (5), 100279 DOI: 10.1016/j.xcrm.2021.100279
Geles, Gao, Giannakou et al
Cell Rep Med (2021) 2 (5), 100279 DOI: 10.1016/j.xcrm.2021.100279
Cited by 32
AI Summary: In this study, OriGene products including the NOTCH3 expression plasmid (RC208445) and NOTCH3 shRNA (TL309386) were used to manipulate NOTCH3 levels in cells, enabling the authors to investigate the effects of NOTCH3-targeted antibody drug conjugates on tumor regression and cell death.
NOTCH3-targeted antibody drug conjugates regress tumors by inducing apoptosis in receptor cells and through transendocytosis into ligand cells
Geles, Gao, Giannakou et al
Cell Reports Medicine (2021) 2 (5), 100279 DOI: 10.1016/j.xcrm.2021.100279
Geles, Gao, Giannakou et al
Cell Reports Medicine (2021) 2 (5), 100279 DOI: 10.1016/j.xcrm.2021.100279
Cited by 32
AI Summary: OriGene products—including Human NOTCH3 (Cat# RC224711), Mouse NOTCH3 (Cat# MR212149), Human NOTCH1 (Cat# RC211365), Mouse NOTCH1 (Cat# MR212150), and Human DLL4 (Cat# RC212628)—were used as recombinant DNA constructs to study the roles of NOTCH3 and DLL4 in the development and testing of NOTCH3-targeted antibody drug conjugates in this research.
Snippet: 878_m1) ThermoFisher Scientific Cat # 433182 B2M (Hs00984230_m1) ThermoFisher Scientific Cat # 433182 ------------------------- Recombinant DNA ------------------------- Human NOTCH3 Origene Cat#RC224711 Mouse NOTCH3 Origene Cat#MR212149 Human NOTCH1 Origene Cat#RC211365 Mouse NOTCH1 Origene Cat#MR212150 Human DLL4 Origene Cat#RC212628 pGL4.27 [Luc2P/
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
