Chd4 (NM_145979) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR212098
Chd4 (Myc-DDK-tagged) - Mouse chromodomain helicase DNA binding protein 4 (Chd4)
$1,609.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Chd4 |
| Synonyms | 9530019N15Rik; AA617397; BC005710; D6Ertd380e; Mi-2beta; mKIAA4075 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_145979 |
| ORF Size | 5745 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_145979.2, NP_666091.1 |
| RefSeq Size | 6438 bp |
| RefSeq ORF | 5748 bp |
| Locus ID | 107932 |
| UniProt ID | Q6PDQ2 |
| Cytogenetics | 6 59.28 cM |
| MW | 218.2 kDa |
| Summary | Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
KSHV episome tethering sites on host chromosomes and regulation of latency-lytic switch by CHD4
Kumar, Lyu, Yanagihashi et al
Cell Rep (2022) 39 (6), 110788 DOI: 10.1016/j.celrep.2022.110788
Kumar, Lyu, Yanagihashi et al
Cell Rep (2022) 39 (6), 110788 DOI: 10.1016/j.celrep.2022.110788
Cited by 52
AI Summary: OriGene contributed to this study by providing the pLenti-Flag-mCHD4-myc plasmid, which was used to investigate the role of CHD4 in regulating the KSHV latency-lytic replication switch.
Snippet: with pLenti-Flag-mCHD4-myc WT or pLenti-Flag-mCHD4-myc mutant using Lipofectamine 2000 reagent according to the manufacturer’s protocol. pLenti-Flag-mCHD4-myc was obtained from a commercial source (Origene, MD, USA). Point mutations were inserted by synthesizing DNA fragments that contain intended mutations and replaced at SexAI-AgeI restriction sites with In-FusionHD (Takara Bio, Japan). At 24
Showing 1-1 of 1 papers.
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in shipping.
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