Chd4 (NM_145979) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG212098
Chd4 (tGFP-tagged) - Mouse chromodomain helicase DNA binding protein 4 (Chd4)
  $2,009.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Chd4
Synonyms 9530019N15Rik; AA617397; BC005710; D6Ertd380e; Mi-2beta; mKIAA4075
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145979
ORF Size 5745 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145979.2, NP_666091.1
RefSeq Size 6438 bp
RefSeq ORF 5748 bp
Locus ID 107932
UniProt ID Q6PDQ2
Cytogenetics 6 59.28 cM
Summary Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145979" in other vectors (2)
SKU Description Size Price
MC225032 Chd4 (untagged) - Mouse chromodomain helicase DNA binding protein 4 (Chd4), (10ug) 10 ug
$1,771.00
MR212098 Chd4 (Myc-DDK-tagged) - Mouse chromodomain helicase DNA binding protein 4 (Chd4) 10 ug
$1,609.00

Plasmid Map for MG212098

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Cloning scheme for MG212098

Circular map for MG212098

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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