Nos3 (BC052636) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR211798
Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765)
$589.00
$1,046.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Nos3 |
| Synonyms | eNOS, ecNOS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC052636 |
| ORF Size | 3606 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC052636.1 |
| RefSeq Size | 3982 bp |
| RefSeq ORF | 3608 bp |
| Locus ID | 18127 |
| Cytogenetics | 5 11.32 cM |
| MW | 146 kDa |
| Summary | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC052636" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223060 | Nos3 (untagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), (10ug) | 10 ug |
$1,152.00
|
|
| MG211798 | Nos3 (tGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) | 10 ug |
$1,246.00
|
|
| MR211798L3 | Lenti ORF clone of Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) | 10 ug |
$1,346.00
|
|
| MR211798L4 | Lenti ORF clone of Nos3 (mGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) | 10 ug |
$1,346.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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