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Nos3 (BC052636) Mouse Untagged Clone

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SKU
MC223060
Nos3 (untagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
  $1,152.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nos3
Synonyms eNOS, ecNOS
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC052636
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACTTGAAGAGTGTGGGCCAGGAGCCTGGGCCACCCTGTGGCCTAGGGCTCGGGCTGGGTTTAG
GGCTGTGCGGCAAGCAGGGCCCAGCCTCTCCAGCACCGGAGCCTAGCCAGGCGCCAGCACCCCCGTCCCC
AACCCGACCAGCACCAGACCACAGCCCCCCGCTAACCCGGCCCCCAGACGGACCCAGGTTTCCTCGAGTA
AAGAATTGGGAAGTGGGCAGCATCACCTACGACACCCTCAGTGCCCAGGCTCAGCAGGATGGGCCCTGTA
CCTCAAGACGCTGCTTGGGATCCCTGGTGTTTCCAAGGAAGTTACAGAGCCGGCCCACCCAGGGCCCTTC
ACCCACTGAGCAGCTATTGGGTCAAGCCCGGGACTTCATCAATCAGTACTATAACTCCATCAAAAGGAGT
GGCTCCCAGGCTCATGAGCAGCGGCTTCAGGAAGTGGAGGCTGAGGTGGCAGCCACAGGCACCTACCAGC
TCCGGGAGAGCGAGCTGGTGTTTGGGGCCAAGCAGGCCTGGCGCAATGCTCCCCGCTGTGTGGGCCGGAT
CCAGTGGGGAAAGCTGCAGGTATTTGATGCTCGGGACTGCAGGACTGCACAGGAAATGTTCACCTACATC
TGTAACCACATTAAATACGCAACAAATAGAGGCAATCTTCGTTCAGCCATCACAGTGTTCCCCCAGCGCT
GCCCTGGCCGGGGAGACTTCCGGATCTGGAACAGCCAGCTGATACGCTATGCGGGCTATAGGCAGCAGGA
TGGCTCCGTGCGAGGGGACCCCGCCAACGTGGAGATCACTGAGCTCTGTATCCAACATGGCTGGACCCCA
GGAAATGGCCGCTTTGATGTGCTGCCCCTGTTACTCCAGGCTCCTGATGAGCCCCCAGAACTCTTCACTC
TGCCCCCAGAGATGGTCCTCGAGGTGCCTCTGGAGCACCCCACGCTCGAGTGGTTTGCTGCCCTTGGCCT
GCGCTGGTATGCCCTCCCAGCTGTGTCCAACATGCTGCTAGAAATCGGGGGCCTGGAGTTTCCTGCTGCC
CCTTTCAGCGGCTGGTACATGAGTTCAGAGATTGGCATGAGGGACCTGTGTGACCCTCACCGCTACAACA
TACTTGAGGATGTGGCTGTGTGCATGGATCTGGACACCAGGACAACCTCATCCCTGTGGAAAGACAAGGC
AGCGGTGGAAATTAATGTGGCCGTGTTGCACAGTTACCAGCTGGCCAAAGTGACCATAGTGGACCACCAC
GCCGCCACAGCCTCCTTCATGAAGCACCTGGAAAATGAGCAGAAGGCCAGAGGGGGCTGCCCTGCCGATT
GGGCCTGGATTGTGCCCCCCATCTCAGGCAGCCTAACTCCTGTCTTCCATCAAGAGATGGTCAACTATTT
CCTGTCCCCTGCCTTCCGCTACCAGCCAGACCCCTGGAAGGGAAGTGCAGCAAAGGGGGCAGGCATCACC
AGGAAGAAGACCTTTAAGGAAGTAGCCAATGCAGTGAAGATCTCTGCCTCACTCATGGGCACGGTGATGG
CGAAGCGTGTGAAGGCAACCATTCTGTATGGCTCTGAGACTGGCCGGGCCCAGAGCTACGCACAGCAGCT
GGGAAGACTCTTCCGGAAGGCGTTTGATCCCCGGGTCCTGTGCATGGATGAGTATGATGTGGTGTCCCTA
GAGCACGAGGCACTGGTGTTGGTGGTGACCAGCACATTTGGCAATGGGGATCCTCCGGAGAATGGAGAGA
GCTTTGCAGCAGCGCTCATGGAAATGTCAGGCCCGTACAACAGCTCCCCTAGGCCTGAGCAGCACAAGAG
CTACAAAATCCGATTCAACAGTGTCTCCTGCTCAGACCCACTGGTATCCTCTTGGCGGCGCAAGAGGAAG
GAGTCTAGCAACACAGACAGTGCAGGAGCCCTGGGCACCCTCAGGTTCTGTGTGTTTGGGCTGGGCTCCC
GAGCATACCCCCACTTCTGTGCCTTTGCTCGAGCGGTGGACACAAGGCTGGAGGAGCTGGGCGGGGAGCG
ACTACTGCAGCTGGGCCAAGGTGATGAGCTCTGTGGCCAGGAGGAGGCTTTCCGAGGCTGGGCCCAGGCC
GCCTTCCAGGCTGCCTGTGAAACCTTCTGTGTGGGAGAAGATGCCAAAGCTGCTGCCCGAGATATCTTCA
GCCCCAAACGCAGCTGGAAGCGCCAGAGGTACCGGCTGAGTACCCAGGCTGAGAGCCTGCAATTACTACC
AGGGCTGACTCACGTGCACAGGCGGAAGATGTTCCAGGCTACAATCCTCTCTGTGGAAAACCTACAGAGC
AGCAAATCCACCCGAGCCACGATCCTGGTGCGTCTGGACACCGGAGGCCAGGAGGGACTGCAGTACCAGC
CAGGGGACCACATAGGTGTGTGCCCACCCAACCGTCCTGGCCTAGTGGAGGCACTGCTGAGCCGAGTGGA
GGACCCTCCGCCATCCACAGAACCTGTGGCTGTGGAACAACTGGAGAAAGGCAGCCCTGGTGGCCCTCCC
CCCGGCTGGGTACGGGACCCCCGGCTACCCCCATGTACGCTGCGGCAGGCTCTCACCTACTTCCTGGACA
TCACTTCCCCGCCTAGTCCTCGCCTCCTTCGACTGCTCAGCACCCTGGCAGAAGAGTCCAGCGAACAGCA
GGAGCTAGAGGCTCTCAGCCAGGACCCCCGGCGCTACGAAGAATGGAAGTGGTTCAGCTGCCCCACACTG
CTAGAGGTGCTGGAGCAATTTCCTTCAGTGGCACTGCCTGCCCCGCTGATCCTCACCCAGCTGCCCTTGC
TCCAGCCCCGGTACTACTCTGTCAGTTCAGCACCCAGCGCCCACCCAGGAGAGATCCACCTCACCATAGC
TGTGCTGGCTTACAGAACCCAGGATGGGCTGGGCCCTCTGCACTACGGTGTCTGCTCCACGTGGATGAGC
CAGCTCAAGGCGGGAGATCCAGTGCCCTGCTTCATCAGGGGGGCTCCCTCCTTCCGGCTGCCACCTGATC
CTAACTTGCCCTGCATCCTGGTGGGCCCAGGGACTGGCATTGCACCCTTCCGGGGATTCTGGCAAGACAG
ACTACACGACATTGAGATCAAAGGGCTACAACCTGCCCCCATGACTTTGGTGTTTGGCTGCCGATGCTCC
CAACTGGACCATCTCTACCGGGACGAGGTACTGGACGCCCAGCAGCGTGGGGTGTTTGGACAAGTCCTCA
CCGCCTTTTCCAGGGATCCTGGCAGCCCCAAGACCTACGTGCAAGACCTCCTGAGGACAGAGCTAGCCGC
GGAGGTTCACCGTGTGCTGTGCCTTGAGCAAGGACATATGTTTGTCTGCGGCGATGTCACTATGGCAACC
AGCGTCCTGCAAACCGTGCAGAGAATTCTGGCAACAGAGGGCGGCATGGAGCTGGATGAAGCCGGTGACG
TCATCGGCGTGCTGCGGGATCAGCAACGCTACCACGAGGACATTTTCGGACTCACATTGCGCACCCAGGA
GGTGACAAGCCGCATACGCACCCAGAGCTTTTCTTTGCAGGAGCGACAGCTGAGGGGCGCAGTGCCCTGG
TCCTTTGACCCGCCTGGCCCAGAAATACCTGGTTCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC052636
Insert Size 3609 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052636, AAH52636
RefSeq Size 3982 bp
RefSeq ORF 3608 bp
Locus ID 18127
Cytogenetics 5 11.32 cM
Summary Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.UniProtKB/Swiss-Prot Function
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"BC052636" in other vectors (4)
SKU Description Size Price
MG211798 Nos3 (tGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) 10 ug
$1,246.00
MR211798 Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) 10 ug
$589.00 $1,046.00
MR211798L3 Lenti ORF clone of Nos3 (Myc-DDK-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) 10 ug
$1,346.00
MR211798L4 Lenti ORF clone of Nos3 (mGFP-tagged) - Mouse nitric oxide synthase 3, endothelial cell (cDNA clone MGC:58949 IMAGE:6530765) 10 ug
$1,346.00

file-alt Citations

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