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Pom121 (NM_148932) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211792
Pom121 (Myc-DDK-tagged) - Mouse nuclear pore membrane protein 121 (Pom121)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,044.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pom121
Synonyms 2610027A18Rik; C80273; mKIAA0618
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211792 representing NM_148932
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCCGGCGGCTGCGGCGGCTGACGGAGGCGAGCGGCGGCGGCCCCCGTTGGGCGGACGGGAAGGCC
GGAGCCGGGCCCGCGGCTACGGAGGACCGGCGGGTGCTGCGGCCCTCGGCCTAGCGCTGCTCGGCCTCGC
GCTCTACCTGGTGCCCGCGGCGGCCGCGCTGGCCTGGCTGGCCGTGGGGGCGAGCGCGGCCTGGTGGGGC
CTGAGCCGAGAACCCCGCGGCCCGAGGGCCCTGTCGTCCTTCGTGCGCGACGCGCGCCGTCACCCGCGGC
CAGCTCTCACCGCTTCACCGCCTCCGGCCAAGTCACCAGTCAACGGTAGCCTCTGCGAACCCCGCAGCCC
GCTGGGAGGCCCCGACCCGGCTGAGCTCTTACTCATGGGCAGCTACCTGGGCAAGCCCGGCCCGCCCGAG
CCTGCCCTCCGGCAGGACCCAAGGGAGAGGCCGGGGCGCCGCCCACCCGCACGCTCCCCGCCTCCGGCCT
CGGCGGTCCAGCGAGTCCACCACGTCTACCCCGCGCTCCCCACCCCGCTTTTGCGACCCTCCCGGAGGCC
GCCCCACCGAGATTGTGGGCCTTTGTCCAGTCGGTTTGTTATAACACCCCGAAGACGATATCCCATTCAG
CAAGCCCAGTATTCCTTGCTTGGGGCACTTCCAACCGTCTGCTGGAATGGTGGCCATAAGAAGGCTGTGC
TGTCTCCACGGAACTCCAGGATGGTGTGCAGCCCAGTGACAGTGAGGATTGCCCCTCCAGACAGCAAGCT
ATTCCGCTCATCAATGTCAGAGCAGATACTCGATACAACACTGTCTTCACCATCAAGTAATGCCCCAGAT
CCTTGTGCAAAGGAGACTGTCCTGAACGCCCTCAAGGAGAAGAAGAAAAGAACAGTGGCGGAGGAAGACC
AGCTGCACCTTGATGGCCAGGAAAACAAGAGAAGGCGCCATGATAGCGGTGGGAGCGGACATTCAGCATT
CGAGCCCCTTGTGGCCAACGGAGTCCCTGCTGCATTTGTGCCTAAGCCTGGCTCCCTGAAGAGAAGTCTG
GCTTCCCAGAGCTCAGATGACCACTTGAACAAACGGTCTCGCACCTCCTCTGTGAGCTCCCTGGCTAGTG
CGTGCACAGGAGGCATCCCCAGTTCTAGCCGCAATGCCATCACCAGCTCCTACAGCTCCACCCGTGGCAT
CTCACAGCTGTGGAAGAGGAGTGGTCCCACATCTTCTCCCTTCTCCAGTCCAGCTTCCTCCCGCTCCCAG
ACACCAGAGAGGCCAGCCAAGAAGACAAGAGAGGAGGAGCCATGTCAGCAGTCCAGTTCTTCACCTCCCT
TGGTGACAGACAAGGAGTCCCCAGGAGAAAAGGTTACAGATACAACCACTGGGAAGCAGCAGAGCTCATG
GACTTCCCCACCCACACCTGGCAGCTCCGGGCAACGCAAACGCAAGATCCAGCTGCTGCCCTCCCGGCGA
GGGGACCAGCTCACCTTGCCTCCACCCCCAGAGCTTGGCTATTCCATCACTGCTGAAGACCTGGACATGG
AGAGGAAAGCCTCACTGCAATGGTTTAACAAGGTCTTGGAGGACAAGCCAGATGATGCTTCCGCCTCAGC
CACTGATGGCCCCCCTTCCACCAGCCCTCCCTTCACATTCACCCTGCCCGCTGTGGGGCCTGCTGCCTCG
CCAGCCTCCCTCCCGGCTCCCAGCTCTAACCCACTGTTGGAGAGCTTGAAGAAGATGCAGGAGTCTCCAG
CCCCATCATCCTCGGAACCTGCTGAGGCAGCAACAGTTGCAGCCCCTTCACCTCCGAAGACACCCAGCCT
CTTGGCCCCTCTTGTCTCACCACTGGCAGGGCCCCTAGCCAGCACCTCCTCAGACTCCAAACCTGCAGCC
ACCTTCCTGGGGCTGGCTTCTGCTTCGTCTATAACCCCACTCACTGACAGCAAGTCCTCTGGAGTCTCTC
AGGCTGAGCAGTCTGTCAGCACCCCAGCCTCCACAGCCTCCTCCCCGACCCCAAAGCCCAGCATGCTGTT
TGGGATGCTGAGTCCACCTGCCAGCTCTTCCTCCCTTGCCACTCCAGCTCCGGCTTGTGCTTCTCCCATG
TTTAAGCCCATTTTTCCAGCCACACCTAAAAGTGAGAGTGATAGCCCCTTACCAAGTAGCTCCTCAGCCG
CCACTACAGCTTCATCCAGCACCGCCCCTCCCACAGCAGCCAGCACCACACCCACGTTCAAGCCCATTTT
TGACAAGATGGAGCCATTCACAGCCATGCCCCTGTCAACTCCCTTCTCCCTGAAGCAGACAACTGCTACA
GCCACCACTACAGCCACATCAGCTCCTCTCTTTACTGGCCTGGGCACTGCCACTTCCACGGTGGCCTCTG
GCACCGCAGCTTCTGCCTCAAAGCCTGTCTTTGGCTTTGGAGTGACCACTGCAGCGAGTACTGCCAGCAG
TACCATGACTTCCACTTCCCAGTCAGTTCTGTTCGGGGGAGCCCCCCCTGTTACTACTTCTAGCTCTGCC
CCAGCTCTGGCCTCCATCTTCCAGTTTGGCAAGCCTCTTGCCCCAGCAGCATCTGCAGCAGGCACCTCCT
TTAGCCAGCCCCTTGCCAGCTCTACCCAGACTGCTGCCAGCAATAGTGGCTTCAGTGGTTTTGGCAGCAC
CCTCACCACCTCCACCTCGGCCCCTGCCACCACCAGTCAGCCCACGCTGACCTTCAGCAACACTGTCACC
CCCACCTTCAACATTCCCTTTAGCTCCAGTGCCAAGCCTGCGCTCCCAACCTACCCTGGAGCCAACTCAC
AGCCCACATTTGGAGCCACCGATGGGGCCACCAAGCCAGCACTTGCCCCAAGTTTTGGCAGCTCGTTCAC
TTTCGGCAACTCTGTGGCCTCAGCCCCATCGGCAGCCCCAGCACCAGCCACCTTTGGCAGTGCTGCACAG
CCAGCTTTCGGAGGGTTGAAAGCCGCAGCCTCCACCTTCGGCGCCCCTGCCAGCACTCAGCCAGCTTTTG
GTAGCACCACGTCTGTGTTCTCCTTTGGGTCAGCTACCACATCTGGCTTTGGTGCTGCTGCCACCGCTGC
TACCACCACACAGACCACCAACAGTGGGAGCAGCAGCTCTCTGTTTGGCAGCTCTGCTCCATCCCCATTC
ACATTCGGTGGCTCAGCAGCTCCTGCTGGCAGTGGAGGCTTTGGCCTTAGTGCTACCCCAGGCACCAGCT
CCACCTCTGGAACTTTCAGCTTTGGATCTGGACAGAGTGGGACCCCGGGCACCACCACCTCCTTTGGAAG
TCTGAGTCAGAACACCCTGGGCGCACCCAGCCAGGGCTCACCCTTTGCCTTCAGTGTGGGCAGTACACCT
GAAAGCAAGCCTGTGTTTGGAGGCACATCTACACCTACTTTCGGGCAGAGTGCCCCTGCCCCCGGCGTAG
GCACCACAGGGAGCAGCCTCTCATTTGGGGCCTCTTCAACACCTGCCCAAGGCTTTGTTGGAGTTGGACC
TTTTGGATCAGCAGCTCCTTCGTTTTCCATTGGTGCGGGATCCAAGACCCCAGGGGCTCGACAGCGACTT
CAGGCCCGAAGGCAGCACACCCGCAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211792 representing NM_148932
Red=Cloning site Green=Tags(s)

MSPAAAAADGGERRRPPLGGREGRSRARGYGGPAGAAALGLALLGLALYLVPAAAALAWLAVGASAAWWG
LSREPRGPRALSSFVRDARRHPRPALTASPPPAKSPVNGSLCEPRSPLGGPDPAELLLMGSYLGKPGPPE
PALRQDPRERPGRRPPARSPPPASAVQRVHHVYPALPTPLLRPSRRPPHRDCGPLSSRFVITPRRRYPIQ
QAQYSLLGALPTVCWNGGHKKAVLSPRNSRMVCSPVTVRIAPPDSKLFRSSMSEQILDTTLSSPSSNAPD
PCAKETVLNALKEKKKRTVAEEDQLHLDGQENKRRRHDSGGSGHSAFEPLVANGVPAAFVPKPGSLKRSL
ASQSSDDHLNKRSRTSSVSSLASACTGGIPSSSRNAITSSYSSTRGISQLWKRSGPTSSPFSSPASSRSQ
TPERPAKKTREEEPCQQSSSSPPLVTDKESPGEKVTDTTTGKQQSSWTSPPTPGSSGQRKRKIQLLPSRR
GDQLTLPPPPELGYSITAEDLDMERKASLQWFNKVLEDKPDDASASATDGPPSTSPPFTFTLPAVGPAAS
PASLPAPSSNPLLESLKKMQESPAPSSSEPAEAATVAAPSPPKTPSLLAPLVSPLAGPLASTSSDSKPAA
TFLGLASASSITPLTDSKSSGVSQAEQSVSTPASTASSPTPKPSMLFGMLSPPASSSSLATPAPACASPM
FKPIFPATPKSESDSPLPSSSSAATTASSSTAPPTAASTTPTFKPIFDKMEPFTAMPLSTPFSLKQTTAT
ATTTATSAPLFTGLGTATSTVASGTAASASKPVFGFGVTTAASTASSTMTSTSQSVLFGGAPPVTTSSSA
PALASIFQFGKPLAPAASAAGTSFSQPLASSTQTAASNSGFSGFGSTLTTSTSAPATTSQPTLTFSNTVT
PTFNIPFSSSAKPALPTYPGANSQPTFGATDGATKPALAPSFGSSFTFGNSVASAPSAAPAPATFGSAAQ
PAFGGLKAAASTFGAPASTQPAFGSTTSVFSFGSATTSGFGAAATAATTTQTTNSGSSSSLFGSSAPSPF
TFGGSAAPAGSGGFGLSATPGTSSTSGTFSFGSGQSGTPGTTTSFGSLSQNTLGAPSQGSPFAFSVGSTP
ESKPVFGGTSTPTFGQSAPAPGVGTTGSSLSFGASSTPAQGFVGVGPFGSAAPSFSIGAGSKTPGARQRL
QARRQHTRKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_148932
ORF Size 3600 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_148932.2, NP_683734.2
RefSeq Size 5602 bp
RefSeq ORF 3603 bp
Locus ID 107939
UniProt ID Q8K3Z9
Cytogenetics 5 G2
MW 121 kDa
Summary Essential component of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. When overexpressed in cells induces the formation of cytoplasmic annulate lamellae (AL) (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_148932" in other vectors (4)
SKU Description Size Price
MC223907 Pom121 (untagged) - Mouse nuclear pore membrane protein 121 (Pom121), (10ug) 10 ug
$1,150.00
MG211792 Pom121 (tGFP-tagged) - Mouse nuclear pore membrane protein 121 (Pom121) 10 ug
$1,244.00
MR211792L3 Lenti ORF clone of Pom121 (Myc-DDK-tagged) - Mouse nuclear pore membrane protein 121 (Pom121) 10 ug
$1,344.00
MR211792L4 Lenti ORF clone of Pom121 (mGFP-tagged) - Mouse nuclear pore membrane protein 121 (Pom121) 10 ug
$1,344.00

Plasmid Map for MR211792

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211792
Circular map for MR211792

file-alt Citations

Sigma-1R-Pom121 axis preserves nuclear transport and integrity in poly-PR-induced C9orf72 ALS
Lin, Wu, Fu et al
Neurobiol Dis (2025) 212, 106992 DOI: 10.1016/j.nbd.2025.106992
Cited by 6
AI Summary: OriGene products Pom121-myc/DDK (MR211792) and Atf3-myc/DDK (MR201634) were used in this study to overexpress Pom121 and ATF3, enabling the authors to demonstrate their protective roles against poly-PR-induced nuclear transport defects and toxicity in C9orf72 ALS models.
Snippet: … F: ACA AGT CGG AGA ACG TGC AGG A R: GTT GTC CGA GTA GGT GGT GAA G Recombinant DNA eGFP-PR42 This paper N/A eGFP-GR50 This paper N/A Pom121-myc/DDK OriGene Technologies, Inc. MR211792 Atf3-myc/DDK OriGene Technologies, Inc. MR201634 HA-SigmaR1 Sino Biological, Inc. MG57873-CY AAV-hsyn-eGFP-PR42 This paper N/A Software and algorithms Fiji ImageJ ImageJ https://imagej.net/ij/ Prism 10 GraphPad https://www.graphpad.com/features QuPath software QuPath https://qupath.github.io Biorender Biorender https://biorender.com/
Nucleoporin POM121 signals TFEB-mediated autophagy via activation of SIGMAR1/sigma-1 receptor chaperone by pridopidine
Wang, Wu, Yasui et al
Autophagy (2022) 19 (1), 126-151 DOI: 10.1080/15548627.2022.2063003
Cited by 68
AI Summary: In this study, OriGene products pCMV6-MYC/DDK (PS100001) and pCMV6-POM121-MYC/DDK (MR211792) were used to overexpress tagged POM121 and control proteins in cells, enabling the authors to investigate the role of POM121 in SIGMAR1-mediated autophagy and TFEB nuclear transport.
Snippet: AGGCCCGA-3’ and 5’-CGCTAGAATGCCGTGGGCCG-3’ mouse SIGMAR1 GenScript SC1948-459   pCMV3-HA-SIGMAR1/Sigma-1 receptor mouse Sino Biological MG57873-NY   pCMV6-MYC/DDK   Origene Technologies Inc PS100001   pCMV6-POM121-MYC/DDK mouse Origene Technologies Inc MR211792   pCMV3-SIGMAR1/Sigma-1 receptor-GFP mouse Origene Technologies Inc MG57873-A;pCMV3-HA (Sino Biological, CV017), pCMV3-HA-SIGMAR1/Sigma-1 receptor (Sino Biological, MG57873-NY), pCMV6-MYC/DDK (Origene Technologie, Inc., PS100001) or pCMV6-POM121-MYC/DDK (Origene Technologies, Inc., MR211792). …
Showing 1-2 of 2 papers.
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