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Spag5 (NM_017407) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211734
Spag5 (Myc-DDK-tagged) - Mouse sperm associated antigen 5 (Spag5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $1,014.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spag5
Synonyms AI874642; D11Bhm180e; Deepest; MAP126; Mastrin; S17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211734 representing NM_017407
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGAGGGTGAAAACACTGAATCTCGGTTTGTCGCCTTCGCCCCAAAAGGGGAAGCCAGCTATGAGTA
CTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCTCGCCGATTCAGGGAAAGGTCCCTCTATGATCTC
CGCGCTGACCCCATACCTGTGCAGGCTGGAGCTGAAGGAAAGATGCAACAACTCATCTCCAGTGGATTTT
ATCAATACTGAGAACAACTTCCTTTCAGAACAGTTCAGCCATCCTTCAACGCACATAGAAGCTTGCCAGC
GTGAATCCGATCCAACTCCTGAAAGCAACTCTCTTTTCCATACTTTGGAGGAAGCAATAGAAACAGTGGA
TGACTTTGTCGTGGACCCAAGAGATGATAGTATAGTGGAGTCCATGGTTCTTCTGCCCTTTTCATTAGGG
CAGCAGCAAGACCTGATGCTCCAGGCTCACTTAGATACCACAGCAGAGAGAACTAAAAGCTCTCTAAATG
AATCTTTGGGGCTAGAAGATCTGGTGGGGAAGGAGGTGGCACCTTGTGTGGAAGACAGCCTAACAGAAAT
TGTTGCTATTAGGCCCGAGCAACCTACATTTCAGGACCCTCCGTTAGGACCCAGTGACACTGAAGATGCA
CCTGTGGACTTAGTTCCTTCTGAAAATGTCCTGAATTTCTCTCTGGCTCGTCTTTCTCCTTCAGCTGTCC
TGGCCCAGGATTTCTCTGTCGATCATGTCGATCCAGGGGAGGAAACCGTAGAGAACAGAGTCCTACAGGA
AATGGAAACGAGCTTCCCCACATTCCCTGAGGAGGCCGAATTGGGAGACCAAGCACCTGCTGCAAATGCA
GAAGCCGTCTCACCCTTGTACCTGACATCAAGTCTAGTGGAGATGGGGCCCCGGGAAGCCCCAGGCCCAA
CAGTAGAAGACGCCAGTAGGATTCCTGGCCTTGAGTCAGAGACTTGGATGTCCCCATTGGCCTGGCTAGA
AAAGGGTGTGAATACATCGGTCATGCTACAAAACCTCCGCCAGAGCTTATCCTTTTCTTCTGTGCTTCAG
GATGCTGCCGTTGGCAACACACCCCTCGCCACGTGTTCTGTGGGCACTTCCTTTACTCCTCCAGCACCAC
TGGAGGTAGGCACCAAGGACAGTACTTCAGAGACAGAGCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCT
GGCTACCTTGTCCCGACACGACTTGGAAGAGAACCTGTTGAACTCTCTTGTCTTGCTGGAAGTTCTTTCC
CACCAGCTACAAGCCTGGAAGAGCCAGTTGACTGTCCCTCACCGGGAAGCTCGAGACAGTAGTACACAGA
CTGACAGCTCTCCTTGTGGGGTCACTAAGACACCTAAACATCTTCAGGACAGCAAGGAGATTAGACAGGC
TCTACTGCAAGCCAGGAATGTCATGCAATCATGGGGTCTAGTCTCTGGAGACCTGTTGTCCTTGCTTCAC
CTGTCCCTAACACACGTGCAAGAAGGCAGAGTGACAGTGAGTCAGGAGTCTCAGCGGTCAAAAACCCTGG
TTTCCTCCTGTTCTCGTGTGCTGAAGAAATTGAAGGCAAAGCTGCAGAGCCTCAAAACAGAATGTGAGGA
GGCAAGGCACAGCAAGGAAATGGCCCTTAAAGGCAAGGCTGCGGCAGAGGCAGTACTGGAGGCTTTCCGT
GCACATGCCAGCCAGCGCATCAGCCAACTGGAACAGGGTCTCACATCTATGCAGGAATTCAGAGGCCTTC
TACAGGAGGCACAGACCCAACTGATAGGGCTTCACACAGAACAAAAGGAGCTGGCTCAGCAGACAGTGAG
TCTGAGTTCTGCCTTGCAGCAGGACTGGACATCAGTCCAATTGAATTATGGAATATGGGCAGCTTTGCTG
AGTTGGTCTCGAGAACTCACCAAGAAACTCACAGCCAAGAGCCGGCAGGCCCTTCAGGAACGTGATGCTG
CAATTGAAGAAAAGAAGCAGGTTGTGAAGGAAGTGGAACAAGTCTCTGCCCATTTAGAGGACTGTAAAGG
TCAAATCGAACAACTGAAGTTGGAAAATAGTCGCCTTACTGCAGATCTCTCGGCTCAGCTGCAGATTCTG
ACTAGCACAGAGAGTCAGCTAAAGGAAGTACGGAGTCAGCATTCCCGCTGTGTCCAGGACCTGGCCGTGA
AGGATGAATTGCTGTGTCAACTTACCCAGAGCAACAAGGAGCAGGCTACTCAATGGCAAAAGGAAGAAAT
GGAACTGAAACACATACAAGCAGAACTGCTACAGCAGCAGGCTGTGTTGGCTAAGGAGGTCCAGGACCTG
AGGGAGACTGTGGAGTTTATAGATGAAGAAAGTCAAGTTGCTCACCGGGAGCTGGGCCAGATTGAGAGTC
AGTTGAAAGTCACGCTAGAGTTACTGCGGGAGCGCAGCCTGCAGTGTGAGACCCTCAGGGACACTGTGGA
CAGCCTGAGGGCTGAGCTGGCCAGCACTGAAGCAAAGCATGAGAAACAGGCCCTAGAGAAGACACACCAG
CATTCTCAAGAGCTGCGGCTACTGGCTGAGCAGCTGCAGAGTCTCACCCTCTTCTTACAGGCAAAACTCA
AGGAGAACAAGGCTGAATCAGAGATCATTCTGCCCAGCACAGGCTCTGCTCCAGCCCAGGAACACCCTCT
GTCCAATGACAGCAGCATCTCAGAACAGACCCCGACAGCAGCAGTAGATGAAGTGCCAGAACCAGCTCCT
GTGCCATTGCTTGGAAGTGTTAAGAGTGCTTTCACCCGAGTAGCCTCAATGGCTTCCTTTCAGCCTACAG
AGACCCCAGACTTGGAGAAGAGCCTGGCAGAAATGAGTACTGTGTTACAAGAGCTTAAGAGCCTGTGTTC
CCTGCTGCAAGAGTCTAAAGAGGAGGCCACTGGGGTCCTGCAGAGGGAAATCTGTGAACTACACTCGAGA
CTACAGGCCCAAGAAGAAGAGCATCAGGAAGCCCTGAAGGCAAAGGAAGCAGACATGGAGAAGCTGAACC
AGGCCTTGTGCTTGCTCCGCAAGAATGAGAAGGAGCTCCTGGAAGTGATACAGAAGCAGAACGAGAAGAT
CCTGGGGCAAATAGACAAGAGCGGCCAGCTCATAAACCTCAGAGAGGAGGTGACCCAGCTCACACAGTCA
CTTCGGCGTGCAGAGACAGAGACTAAAGTGCTCCAGGAAGCCCTGGAAGGCCAGCTAGATCCCAGCTGCC
AGCTGATGGCTACTAACTGGATCCAGGAAAAAGTGTTTCTCTCACAGGAGGTGAGCAAGCTGAGGGTTAT
GTTCCTGGAGATGAAAACTGAGAAGGAACAGCTGATGGACAAGTATCTGAGCCATAGGCACATCCTGGAG
GAGAATCTTCGGCGCTCTGACACAGAGTTAAAGAAACTCGATGACACAATTCAGCATGTCTATGAGACTC
TGTTGTCTATCCCAGAGACTATGAAGAGTTGCAAGGAGTTACAAGGATTGCTAGAATTTCTGAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211734 representing NM_017407
Red=Cloning site Green=Tags(s)

MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDF
INTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLG
QQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDA
PVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANA
EAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQ
DAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLS
HQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLH
LSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFR
AHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALL
SWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQIL
TSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDL
RETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQ
HSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAP
VPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSR
LQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLINLREEVTQLTQS
LRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLSQEVSKLRVMFLEMKTEKEQLMDKYLSHRHILE
ENLRRSDTELKKLDDTIQHVYETLLSIPETMKSCKELQGLLEFLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_017407
ORF Size 3495 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_017407.3
RefSeq Size 3822 bp
RefSeq ORF 3498 bp
Locus ID 54141
UniProt ID Q7TME2
Cytogenetics 11 46.74 cM
MW 130.4 kDa
Summary Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity).UniProtKB/Swiss-Prot Function
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"NM_017407" in other vectors (4)
SKU Description Size Price
MC202063 Spag5 (untagged) - Mouse sperm associated antigen 5 (Spag5), (10ug) 10 ug
$1,015.00
MG211734 Spag5 (tGFP-tagged) - Mouse sperm associated antigen 5 (Spag5) 10 ug
$1,214.00
MR211734L3 Lenti ORF clone of Spag5 (Myc-DDK-tagged) - Mouse sperm associated antigen 5 (Spag5) 10 ug
$1,314.00
MR211734L4 Lenti ORF clone of Spag5 (mGFP-tagged) - Mouse sperm associated antigen 5 (Spag5) 10 ug
$1,314.00

Plasmid Map for MR211734

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211734
Circular map for MR211734

file-alt Citations

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