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Ankrd27 (BC065093) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211401
Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $909.00
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Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd27
Synonyms MGC25907, Varp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211401 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTATATGATGAAGATCTCCTGAAAAACCCTTTCTACCTGGCCCTTCAGAAGTGGCGCCCCGACT
TGTGCAGCAAGGTGGCCCAGATCCATGGCATTGTCCTAGTGCCTTGCAGAGGAAGCCTGCCCGGCAGTGT
GCAGGCCTCCTGCCAGTTCGAGTCCTATGTTTTGGTACCCACGGAAGGACACTTTCAGACCTTAGATGGA
AAGGCTGTCGTCATTGAAGGAAACAGGATTAAGCTAGGAGCAGGTTTTGCTTGCCTTCTCTCTGTGCCCA
TCCTCTTTGAGGAGACTTTCTACAACGAGAAAGAGGAGAGTTTCAGCATTCTCTGCATTGCTCATCCTTT
GGAGAGGAGAGAGACTTCAGAAGAACCTTCAGCGCCTGCAGATCCTTTCTCCCTGAAAACCATCGAAGAT
GTGAGAGAATTTTTGGGAAGACACTCAGAGAAATTTGACAAGAACATTGCCTCTTTCCACCGGACGTTCC
GAGAATGTGAAAGAAAGAGCCTCCGCCACCACATAGACTCCGTAAATGCTCTCTACACCAAATGCCTCCA
GCAGCTTCTCCGAGACTCTCACCTGAAGGTTCTTGCAAAGCAGGAGGCCCAGATGAACCTGATGAAGCAG
GCCGTAGAGATGTATGTCCATCACGATATTTACGACCTGATCTTTAAATACGTGGGGACCATGGAGGCGA
GCGAGGATGCCGCCTTTAACAAAATCACGAGAAGCCTTCAAGATCTCCAGCAGAAAGACATCGGCGTGAA
ACCCGAGTTCAGCTTCAACATCCCTCGGGCAAAGAGAGAGCTGGGTCAGTTGAACAAGTGTACGTCGCCA
CAGCAGAAGCTGCTGTGCCTGAGGAAGGTGGTCCAGCTCATGACACAATCTCCCAGCCAGAGAGTGAACT
TGGAGACCATGTGTGCCGATGATCTCCTCTCGGTCCTGTTATATCTGCTCGTGAAGACAGAGATCCCTAA
CTGGATGGCAAATTTGAGCTACATCAAAAACTTCAGATTTAGCAGCTCGGCCAAAGATGAGCTAGGATAC
TGCCTGACCTCAGTCGAGGCTGCGATTGAATACATCCGGCAAGGAAGTCTCTCCACGAAGACGCCTGACG
CTGAGGGATTTGGCGACAGACTATTTCTCAAGCAGCGGATGAACTTGCTGTCCCAGATGACATCAACACC
CATCGACTGCCTGTTCAAGCACATCGCCTCTGGTAACCAAAAGGAAGTGGAAAGACTTCTGAGCCAAGAT
GACCAGGACAAAGACGCCATGCAAAAGATGTGCCACCCACTTTGTTCCTGTGAGGACTGTGAGAAGCTTA
TCTCTGGGAGGCTGAATGACCCGTCAGTTGTCACTCCTTTCTCCAGAGATGACAGAGGTCAAACACCCCT
CCATGTGGCTGCCCTCTGTGGTCAGGCATCCCTCATTGACTTCCTGGTCTCCAAAGGTGCCGTAGTGAAC
GCCACAGACTATCATGGGTCCACCCCCTTACATCTGGCATGTCAGAAAGGCTTCCAAAGTGTGACGCTGC
TGCTGCTGCACTACAAGGCCAGCACTGAGGTTCAGGACAACAACGGCAACACCCCGCTGCACCTGGCCTG
CACCTATGGGCAGGAGGATTGCGTGAAGGCCCTGGTCTACTATGATGTCCAGGCCTGCAGATTGGATATT
GGTAATGAGAAAGGAGACACAGCCCTGCACATTGCTGCACGCTGGGGTTACGAGGGCATCATAGAGACAC
TGCTGCAGAATGGAGCCCCCACAGCAGTCCAGAACAGACTGAAGGAAACACCACTCAAGTGTGCTCTGAA
CTCGAAGATTCTGTCTATCATGGAAGCTCATCACCTATCCTCTGACAGGAGGCCAAGGCCTTCTGAGGTT
CCTGCACAGTCCCCTACACGCTCTGTGGATTCCATCAGCCAGGGCTCCTCCACCTCCAGCTTTTCTTCCA
TATCGGTGAGCTTCAGGCAAGAAGAGGTCAAAAAGGACTACAGGGAGAAACTGGCCAGGATTTCTGCCAA
TGGGCTTAGTGTGAATGTGACCAACCAGGACGGCTTCTCTCCCCTGCACATGGCTGCCCTGCATGGCCGC
ACAGACCTCGTCCCCCTCCTGCTGAAGCACGGTGCTTACTCAGGTGCCAGAAACACAAGCCAAGCCGTCC
CGCTCCACCTGGCCTGCCAGCAGGGGCACTTTCAGGTGGCAAAATGTCTCTTAGATTCCAATGCAAAACC
TAATAAAAAGGACCTCAGTGGGAACACACCCCTCATTTGTGCCTGCTCTGCCGGCCACCATGAAGTCGCT
GCGCTGTTGCTACAGCACGGGGCCTCCATCAATGCTTGCAACAACAAAGGCAACACGGCCCTGCATGAGG
CTGTGATGGGAAGGCACACCCTGGTAGTGGAGCTGCTTCTGTTCTACGGGGCGTCGGTTGACATCCTGAA
CAAGAGGCAGTACACGGCGGCGGACTGTGCCGAGCAGGACTCAAAAATAATGGAGTTGCTGCAGGTAGTG
CCAGGCTGTGTTGCGTCTCTAGACAGCGTCGAGGAGGCAGACCACGAGGGTTACGTGACTGTTGAGATCA
GGAGAAAATGGAACCCCAAAATGTACAACCTGCCAGAGGAACCTCTTAGGAGACAGTTTTGCCTCATTAA
CCCGGGGGGTCGGTTCCAGGAGAGGACATCCAGGGAGACTATGGGAAGGGATAGAAGTGTGCCCGATCTT
GCAGGACGTTCTCTTCAGGAGCCAGAGAAGCAAAGAGTCACCGGAAAGCAGAGTGACCTGTCAGACCTGA
GCAGGTATCAGACCTCTGAGGAAGGAAACAAGGGGCTCCCGGAGAGGCCCGTGTCGAGACAGGCTGCTCC
AGGACACCGGCCGATGGTCCGCAGACACACAGTCAATGACGCAGCCATACTCCAGGTCCCAGAGGTGACT
GTCCACCTCACCACCCACGAGGCGAGTGTTCCTCAGTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211401 protein sequence
Red=Cloning site Green=Tags(s)

MALYDEDLLKNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPGSVQASCQFESYVLVPTEGHFQTLDG
KAVVIEGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLERRETSEEPSAPADPFSLKTIED
VREFLGRHSEKFDKNIASFHRTFRECERKSLRHHIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQ
AVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSP
QQKLLCLRKVVQLMTQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSAKDELGY
CLTSVEAAIEYIRQGSLSTKTPDAEGFGDRLFLKQRMNLLSQMTSTPIDCLFKHIASGNQKEVERLLSQD
DQDKDAMQKMCHPLCSCEDCEKLISGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVN
ATDYHGSTPLHLACQKGFQSVTLLLLHYKASTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDI
GNEKGDTALHIAARWGYEGIIETLLQNGAPTAVQNRLKETPLKCALNSKILSIMEAHHLSSDRRPRPSEV
PAQSPTRSVDSISQGSSTSSFSSISVSFRQEEVKKDYREKLARISANGLSVNVTNQDGFSPLHMAALHGR
TDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKKDLSGNTPLICACSAGHHEVA
ALLLQHGASINACNNKGNTALHEAVMGRHTLVVELLLFYGASVDILNKRQYTAADCAEQDSKIMELLQVV
PGCVASLDSVEEADHEGYVTVEIRRKWNPKMYNLPEEPLRRQFCLINPGGRFQERTSRETMGRDRSVPDL
AGRSLQEPEKQRVTGKQSDLSDLSRYQTSEEGNKGLPERPVSRQAAPGHRPMVRRHTVNDAAILQVPEVT
VHLTTHEASVPQS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC065093
ORF Size 2979 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC065093, AAH65093
RefSeq Size 4105 bp
RefSeq ORF 2981 bp
Locus ID 245886
Cytogenetics 7 B2
MW 110.6 kDa
Summary May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics (By similarity). May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation (By similarity). Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7 (PubMed:19403694, PubMed:21187289). Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38 (PubMed:19745841, PubMed:22171327). Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. Required for the colocalization of VAMP7 and Rab21, probably on TGN sites (By similarity). Involved in GLUT1 endosome-to-plasma membrane trafficking; the function is dependent of association with VPS29 (By similarity). Regulates the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes (PubMed:26620560).UniProtKB/Swiss-Prot Function
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"BC065093" in other vectors (4)
SKU Description Size Price
MC220935 Ankrd27 (untagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501), (10ug) 10 ug
$1,001.00
MG211401 Ankrd27 (tGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) 10 ug
$1,109.00
MR211401L3 Lenti ORF clone of Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) 10 ug
$1,209.00
MR211401L4 Lenti ORF clone of Ankrd27 (mGFP-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501) 10 ug
$1,209.00

Plasmid Map for MR211401

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211401
Circular map for MR211401

file-alt Citations

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