Aass (NM_013930) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR211209
Aass (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein
$589.00 $933.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Aass
Synonyms LKR; LKR/SDH; Lo; LOR; LOR/SDH; Lorsdh; SDH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_013930
ORF Size 2778 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013930.4, NP_038958.2
RefSeq Size 3701 bp
RefSeq ORF 2781 bp
Locus ID 30956
UniProt ID Q99K67
Cytogenetics 6 10.27 cM
MW 103 kDa
Summary This gene encodes a bifunctional mitochondrial protein that catalyzes the first two steps in the lysine degradation pathway. The N-terminus contains lysine-ketoglutarate reductase activity and converts lysine to saccharopine, whereas the C-terminus contains saccharopine dehydrogenase activity and converts saccharopine to alpha-aminoadipate semialdehyde. Mutations in a human gene encoding a highly similar protein are associated with familial hyperlysinemia. provided by RefSeq, Jul 2008
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"NM_013930" in other vectors (4)
SKU Description Size Price
MC205647 Aass (untagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$849.00
MG211209 Aass (tGFP-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein 10 ug
$1,133.00
MR211209L3 Lenti ORF clone of Aass (Myc-DDK-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein 10 ug
$1,233.00
MR211209L4 Lenti ORF clone of Aass (mGFP-tagged) - Mouse aminoadipate-semialdehyde synthase (Aass), nuclear gene encoding mitochondrial protein 10 ug
$1,233.00

Plasmid Map for MR211209

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Cloning scheme for MR211209

Circular map for MR211209

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