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Usp4 (BC011341) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211165
Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $838.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Usp4 or BC011341"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Usp4
Synonyms F730026I20Rik; mKIAA4155; Unp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211165 representing BC011341
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGGCCGGGGCAGCCGTGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA
TGGGGACCACGCTCCAACGTGGGGCGCAGTGGTATCTTATTGACAGCCGGTGGTTCAAGCAGTGGAAGAA
GTATGTGGGCTTTGACAGCTGGGACATGTACAATGTCGGGGAGCATAACCTGTTTCCTGGACCGATTGAC
AACTCCGGACTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAGCACTTAATCGATGAGCTGGACTATG
TGCTGGTCCCAGCCGAAGCCTGGAATAAATTGCTGAATTGGTATGGCTGTGTGGAGGGTCAGCAGCCTAT
TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGCTG
AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGACACCATTGCAA
CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAACGTGAAACACGGCTTTGGAACAAATA
CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT
CAGGTGCTAGTAATTGAGCCCCAAAATGAAGATGGCACATGGCCCCGGCAGAGCCTGCAGTCAAATGGAT
CTGGCTTCTCTGCTTCGTATAATTGCCAGGAGCCCCCATCACCTCATATACAGCCTGGCCTCTGTGGACT
TGGAAACCTGGGGAACACCTGCTTCATGAACTCTGCTCTGCAGTGCCTGAGCAACACTGCCCCACTGACT
GAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACCGAGACAACCCTCTGGGGATGAAAGGGGAGA
TTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACTCATGTGGCACCACGGAT
GTTCAAGACGCAAGTGGGACGTTTTGCCCCTCAGTTTTCTGGCTACCAACAGCAAGACTCCCAGGAGCTG
CTAGCCTTTATTCTAGACGGACTGCACGAGGACCTGAACCGCGTAAAGAAGAAGCCTTACCTGGAGCCCA
AGGACGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAACCACAGGCTGAGGAATGA
TTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCGACTTTGGTTTGCCCAGAATGTGCTAAAGTT
TCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAGGATCGGATTATGGAGG
TCTTCCTGGTTCCTGCTGACCCTCAGTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGGC
CATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGAT
GTATATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGATG
ACATTTTTGTGTATGAGGTCTGCAACATGTCCATGGATGGCTCAGAGTGTATCACTCTTCCAGTCTACTT
CAGAGAGAAGAAGTCCAGGCCATCGAGTGCTTCCTCGGGGGCTGTGCTCTATGGACAGCCCCTTCTTGTC
TCTGTCCCTAAGCATAAGCTAACCCTTGAGTCTTTGTACCAGGCTGTTTGTGATCGTATCAGCCGCTACA
TAAAACAGCCTTTGCCTGATGAGTTTCTCAGCTCACCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGAG
TAGCTATGAAGGAGATGAAGAGGAAGAAATGGATCATCAAGAGGAAGGAAAAGAGCAGCTTTCCGAAGTG
GAAGGCAGTGGTGAGGACGATCAGGGAGATGACCATAGCGAGAGCGCCCAAAAGGTGAAAGGCCAGCCGA
GGCACAAGAGGCTCTTTACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAATTCACTGGCAAC
TGACGGGAAACTCCTCAAACTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAGC
CTTTACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACCTGAGCATGTCACAGCCGCAGAAGAAGA
AGAAGGCGGCGGTAGCCCTGCGAGAGTGCATCGAGCTCTTCACCACCATGGAGACCCTTGGGGAGCATGA
CCCCTGGTACTGTCCCACCTGTAAGAAGCACCAGCAGGCAACAAAGAAGTTTGACTTGTGGTCTTTGCCC
AAGATCCTGGTGGTTCACCTCAAGCGTTTCTCCTATAACAGATACTGGCGGGATAAACTTGACACCGTCG
TGGAGTTCCCAGTCAGAGCTCTGAACATGTCCGAGTTTGTCTGTGACCGGTCAGCAAGGCCTTATGTTTA
TGACCTAATTGCTGTGTCCAATCACTATGGAGCCATGGGGGTTGGTCACTACACTGCATATGCGAAGAAC
AGACTGAACGGGAAATGGTATTACTTTGATGATAGCAGTGTGTCCCTGGCCTCTGAGGACCAGATAGTGA
CGAAAGCCGCCTACGTGTTGTTCTATCAGCGTCGGGATGACGAATGCTCCAGCACATCTTCGCTCGGCAG
TTTCCCCGGTTCTGATGGAGGGGTGAAGCTGAGCAGCTCACACCAGGGCATGGGGGATGAGGAGGCTTAC
AACATGGACACCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211165 representing BC011341
Red=Cloning site Green=Tags(s)

MAEGRGSRERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID
NSGLFSDPESQTLKEHLIDELDYVLVPAEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL
KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG
QVLVIEPQNEDGTWPRQSLQSNGSGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTAPLT
EYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQEL
LAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKV
SVTFDPFCYLTLPLPLKKDRIMEVFLVPADPQCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTD
VYNHRFHKIFQMDEGLSHITPRDDIFVYEVCNMSMDGSECITLPVYFREKKSRPSSASSGAVLYGQPLLV
SVPKHKLTLESLYQAVCDRISRYIKQPLPDEFLSSPLEPGACNGSRSSYEGDEEEEMDHQEEGKEQLSEV
EGSGEDDQGDDHSESAQKVKGQPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRS
LYFDEQESEACEKHLSMSQPQKKKKAAVALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLP
KILVVHLKRFSYNRYWRDKLDTVVEFPVRALNMSEFVCDRSARPYVYDLIAVSNHYGAMGVGHYTAYAKN
RLNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQRRDDECSSTSSLGSFPGSDGGVKLSSSHQGMGDEEAY
NMDTN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC011341
ORF Size 2745 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC011341, AAH11341
RefSeq Size 3455 bp
RefSeq ORF 2747 bp
Locus ID 22258
Cytogenetics 9 59.25 cM
MW 126.6 kDa
Summary Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC011341" in other vectors (4)
SKU Description Size Price
MC220766 Usp4 (untagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165), (10ug) 10 ug
$922.00
MG211165 Usp4 (tGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) 10 ug
$1,038.00
MR211165L3 Lenti ORF clone of Usp4 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) 10 ug
$1,138.00
MR211165L4 Lenti ORF clone of Usp4 (mGFP-tagged) - Mouse ubiquitin specific peptidase 4 (proto-oncogene) (cDNA clone MGC:18374 IMAGE:3661165) 10 ug
$1,138.00

Plasmid Map for MR211165

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211165
Circular map for MR211165

file-alt Citations

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