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Psmd2 (NM_134101) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211143
Psmd2 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $832.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Psmd2 or NM_134101"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psmd2
Synonyms 9430095H01Rik; AA407121; TEG-190; Tex190
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211143 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAGGGTGGACGAGACAAGACACCCGTGCAGTCCCAACAGCCCTCCGCGACAACCCCCAGCGGCG
CGGACGAGAAGTCGAGTGGCAAGGAGCGAAGGGATGCCGGGGAAAAAGACAAAGAGCAGGAGCTGTCTGA
GGAGGACAAACAGCTTCAAGATGAACTGGAGATGCTCGTGGAGCGACTCGGGGAGAAAGATACATCTCTA
TATCGACCAGCTCTGGAGGAACTGAGAAGACAAATTCGTTCTTCTACAACTTCCATGACTTCAGTACCCA
AGCCTCTCAAATTTCTGCGTCCACACTATGGCAAATTGAAGGAAATCTATGAGAACATGGCCCCTGGGGA
GAATAAGTGTTTTGCAGCTGACATCATCTCTGTTTTGGCCATGACCATGAGTGGTGAGCGGGAGTGCCTC
AAGTATCGGCTCGTGGGCTCCCAGGAAGAACTGGCGTCATGGGGCCATGAATACGTCAGACATCTGGCAG
GAGAGGTGGCTAAGGAGTGGCAGGAGCTGGATGATGCGGAGAAAGCACAGCGGGAACCACTGCTTACCTT
GGTAAAGGAAATCGTCCCCTACAACATGGCTCACAATGCAGAGCACGAGGCTTGTGACCTGCTGATGGAA
ATTGAGCAGGTGGACATGCTAGAGAAGGACATTGATGAGAACGCTTACGCCAAGGTCTGCCTCTATCTCA
CCAGTTGTGTGAATTATGTACCAGAACCTGAGAACTCTGCCCTCCTGCGCTGCGCCTTGGGCGTGTTCCG
AAAGTTTAGCCGTTTCCCTGAAGCTCTGAGATTGGCACTGATGCTCAATGACATGGAACTGGTAGAAGAC
ATTTTCACTTCCTGCAAAGATGTGGTGGTCCAGAAGCAGATGGCATTCATGCTGGGTCGGCATGGAGTGT
TCCTGGAGCTGAGTGAAGATGTAGAGGAATATGAAGACCTCACAGAAATCATGTCCAATGTCCAGCTCAA
CAGCAACTTCTTGGCTTTAGCGCGGGAGCTGGACATCATGGAGCCCAAGGTGCCTGATGACATCTACAAA
ACCCACCTAGAGAATAACAGGTTTGGTGGCAGTGGCTCTCAAGTGGATTCTGCACGAATGAACCTAGCCT
CCTCTTTTGTAAATGGCTTTGTGAATGCAGCCTTTGGTCAAGATAAACTACTGACTGATGATGGCAACAA
ATGGCTTTACAAGAACAAGGACCATGGGATGCTAAGTGCTGCTGCGTCTCTTGGCATGATTCTGCTGTGG
GATGTGGATGGTGGCCTCACCCAGATTGACAAGTATCTGTACTCCTCTGAGGACTACATCAAGTCAGGAG
CTCTCCTTGCCTGTGGTATCGTGAATTCTGGAGTCCGGAATGAGTGTGATCCTGCCCTGGCACTGCTTTC
AGACTATGTTCTCCATAACAGCAATACCATGAGACTTGGCTCCATCTTTGGGCTAGGTTTGGCCTATGCT
GGCTCTAATCGGGAAGATGTTCTAACACTGCTGCTACCTGTAATGGGAGATTCCAAGTCCAGCATGGAGG
TGGCAGGTGTGACGGCTCTAGCTTGTGGGATGATAGCAGTGGGGTCCTGCAATGGAGATGTCACTTCCAC
CATCCTTCAGACCATCATGGAGAAATCTGAGACTGAGCTCAAGGATACCTATGCTCGTTGGCTTCCTCTT
GGCCTGGGCCTCAATCACTTGGGGAAAGGCGAAGCTATTGAGGCCATCCTGGCTGCCTTGGAGGTGGTGT
CAGAGCCATTCCGCAGTTTTGCCAATACCCTGGTGGATGTATGTGCCTATGCAGGCTCTGGGAATGTGCT
GAAGGTTCAGCAGCTCCTCCACATCTGTAGTGAGCACTTTGACTCTAAGGAAAAGGAGGAAGACAAAGAC
AAGAAGGAAAAGAAGGACAAGGACAAGAAGGAAGCCCCTGCTGACATGGGAGCACATCAGGGAGTAGCTG
TTCTGGGCATTGCACTTATTGCTATGGGGGAAGAGATTGGTGCAGAGATGGCATTGCGAACTTTTGGTCA
CTTGCTGAGATATGGGGAGCCTACCCTCCGTCGCGCTGTACCTTTAGCACTGGCACTAATTTCTGTTTCA
AATCCACGACTCAACATCCTGGATACGCTAAGCAAATTCTCTCATGATGCTGATCCAGAAGTTTCCTATA
ACTCCATCTTTGCTATGGGCATGGTGGGCAGTGGTACCAATAATGCCCGTCTGGCTGCAATGTTGCGCCA
GTTAGCTCAATATCATGCCAAGGACCCCAATAACCTCTTCATGGTGCGCTTGGCACAGGGCTTGACACAT
TTAGGGAAGGGCACACTTACCCTCTGCCCCTATCACAGTGATCGGCAGCTCATGAGTCAAGTAGCGGTGG
CAGGGCTACTCACGGTGCTTGTTTCTTTCCTGGATGTTCGAAACATCATCCTAGGCAAGTCTCACTATGT
CCTGTATGGGCTGGTAGCCGCCATGCAGCCTCGGATGCTGGTTACATTTGATGAGGAGCTGCGACCATTG
CCAGTTTCTGTCCGTGTGGGTCAGGCAGTGGATGTGGTGGGCCAGGCTGGGAAGCCAAAAACAATCACAG
GGTTCCAGACTCATACAACGCCAGTGTTGCTGGCCCATGGGGAGCGGGCAGAGTTGGCCACTGAGGAGTT
TCTTCCTGTCACCCCCATTCTGGAAGGCTTTGTTATTCTTCGGAAGAACCCCAACTATGATCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211143 protein sequence
Red=Cloning site Green=Tags(s)

MEEGGRDKTPVQSQQPSATTPSGADEKSSGKERRDAGEKDKEQELSEEDKQLQDELEMLVERLGEKDTSL
YRPALEELRRQIRSSTTSMTSVPKPLKFLRPHYGKLKEIYENMAPGENKCFAADIISVLAMTMSGERECL
KYRLVGSQEELASWGHEYVRHLAGEVAKEWQELDDAEKAQREPLLTLVKEIVPYNMAHNAEHEACDLLME
IEQVDMLEKDIDENAYAKVCLYLTSCVNYVPEPENSALLRCALGVFRKFSRFPEALRLALMLNDMELVED
IFTSCKDVVVQKQMAFMLGRHGVFLELSEDVEEYEDLTEIMSNVQLNSNFLALARELDIMEPKVPDDIYK
THLENNRFGGSGSQVDSARMNLASSFVNGFVNAAFGQDKLLTDDGNKWLYKNKDHGMLSAAASLGMILLW
DVDGGLTQIDKYLYSSEDYIKSGALLACGIVNSGVRNECDPALALLSDYVLHNSNTMRLGSIFGLGLAYA
GSNREDVLTLLLPVMGDSKSSMEVAGVTALACGMIAVGSCNGDVTSTILQTIMEKSETELKDTYARWLPL
GLGLNHLGKGEAIEAILAALEVVSEPFRSFANTLVDVCAYAGSGNVLKVQQLLHICSEHFDSKEKEEDKD
KKEKKDKDKKEAPADMGAHQGVAVLGIALIAMGEEIGAEMALRTFGHLLRYGEPTLRRAVPLALALISVS
NPRLNILDTLSKFSHDADPEVSYNSIFAMGMVGSGTNNARLAAMLRQLAQYHAKDPNNLFMVRLAQGLTH
LGKGTLTLCPYHSDRQLMSQVAVAGLLTVLVSFLDVRNIILGKSHYVLYGLVAAMQPRMLVTFDEELRPL
PVSVRVGQAVDVVGQAGKPKTITGFQTHTTPVLLAHGERAELATEEFLPVTPILEGFVILRKNPNYDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_134101
ORF Size 2724 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_134101.1, NM_134101.2, NP_598862.1
RefSeq Size 3000 bp
RefSeq ORF 2727 bp
Locus ID 21762
UniProt ID Q8VDM4
Cytogenetics 16 B1
MW 100.2 kDa
Summary Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_134101" in other vectors (4)
SKU Description Size Price
MC222502 Psmd2 (untagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), transcript variant 1, (10ug) 10 ug
$915.00
MG211143 Psmd2 (tGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2) 10 ug
$1,032.00
MR211143L3 Lenti ORF clone of Psmd2 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), transcript variant 1 10 ug
$1,132.00
MR211143L4 Lenti ORF clone of Psmd2 (mGFP-tagged) - Mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (Psmd2), transcript variant 1 10 ug
$1,132.00

Plasmid Map for MR211143

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211143
Circular map for MR211143

file-alt Citations

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