Gria1 (NM_008165) Mouse Tagged ORF Clone

SKU MR211142

Gria1 (Myc-DDK-tagged) - Mouse glutamate receptor, ionotropic, AMPA1 (alpha 1) (Gria1), transcript variant 2

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Gria1
• Synonyms: 2900051M01Rik; Glr-1; Glr1; GluA1; Glur-1; GluR-A; gluR-K1; Glur1; GluRA; HIPA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR211142 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCCGTACATCTTTGCCTTTTTCTGCACCGGTTTTCTAGGTGCGGTTGTGGGTGCCAATTTCCCCAACA; ATATCCAGATAGGGGGATTATTTCCAAACCAACAATCACAGGAACATGCGGCTTTTAGGTTTGCTTTGTC; ACAACTCACGGAGCCCCCCAAGCTGCTTCCCCAGATCGATATTGTGAACATCAGCGACAGCTTTGAGATG; ACTTACCGATTCTGTTCCCAGTTCTCCAAAGGAGTGTACGCCATCTTTGGATTTTATGAACGAAGGACTG; TCAACATGCTGACCTCCTTCTGTGGGGCCCTCCATGTGTGCTTCATCACTCCAAGTTTTCCCGTTGACAC; ATCCAATCAGTTTGTCCTTCAGCTGCGCCCGGAACTACAGGAAGCTCTCATTAGCATTATCGACCATTAC; AAGTGGCAGACTTTTGTCTACATTTATGATGCTGACCGGGGCCTGTCAGTCCTGCAGAGAGTCTTGGATA; CAGCCGCCGAGAAGAACTGGCAGGTGACGGCTGTCAACATTCTAACAACCACGGAGGAAGGATACCGGAT; GCTCTTTCAGGACCTGGAGAAGAAAAAGGAGAGGCTGGTGGTGGTGGACTGTGAATCAGAACGCCTCAAC; GCCATCCTGGGCCAGATTGTGAAGCTAGAAAAGAACGGCATCGGGTACCACTACATCCTCGCCAACCTGG; GCTTCATGGACATTGACTTAAATAAGTTCAAGGAGAGTGGAGCCAATGTGACAGGTTTCCAACTGGTGAA; CTACACAGACACGATCCCAGCCAGAATCATGCAGCAGTGGAGGACAAGTGACGCTCGGGACCACACCAGG; GTGGACTGGAAGAGGCCAAAGTACACTTCTGCTCTTACCTATGATGGTGTGAAGGTGATGGCGGAGGCCT; TCCAGAGCCTGCGGAGGCAGAGGATTGACATATCCCGGCGAGGGAATGCTGGGGACTGTCTGGCTAACCC; AGCTGTGCCCTGGGGCCAAGGGATCGACATCCAGAGAGCCCTGCAGCAGGTGCGCTTTGAAGGTTTGACA; GGAAATGTGCAGTTTAACGAGAAAGGGCGCCGGACCAACTACACCCTCCATGTGATCGAAATGAAGCATG; ATGGAATCCGCAAGATTGGTTACTGGAATGAAGATGATAAATTTGTCCCCGCAGCCACGGACGCTCAGGC; TGGAGGGGACAACTCAAGCGTCCAGAATAGAACCTACATCGTCACGACTATCCTCGAAGATCCTTACGTG; ATGCTTAAAAAGAATGCCAACCAATTTGAAGGCAATGACCGCTATGAGGGCTACTGCGTGGAACTGGCTG; CGGAGATCGCCAAGCACGTGGGCTATTCCTACCGACTTGAGATTGTCAGCGACGGCAAATACGGAGCCCG; GGATCCTGACACAAAGGCCTGGAATGGCATGGTGGGAGAGCTAGTCTATGGAAGAGCAGATGTGGCGGTG; GCCCCCTTGACCATAACCTTGGTCCGGGAGGAAGTCATCGACTTCTCCAAGCCATTCATGAGTTTGGGAA; TCTCCATTATGATTAAGAAGCCACAGAAGTCCAAGCCAGGTGTCTTCTCCTTTCTTGACCCTTTGGCCTA; CGAGATCTGGATGTGTATAGTGTTTGCCTACATTGGAGTGAGCGTCGTCCTCTTCCTGGTCAGCCGTTTC; AGTCCTTATGAATGGCACAGTGAAGAGTTTGAAGAAGGACGAGATCAGACAACCAGTGACCAGTCAAATG; AGTTTGGCATATTCAACAGCCTGTGGTTCTCGCTGGGGGCCTTCATGCAGCAAGGATGTGACATTTCCCC; CAGGTCCCTGTCTGGACGCATCGTCGGCGGTGTCTGGTGGTTCTTCACTTTGATTATCATCTCCTCATAC; ACAGCCAACCTGGCTGCCTTCCTGACTGTGGAAAGGATGGTGTCTCCCATCGAGAGTGCAGAGGACCTGG; CAAAGCAGACGGAAATTGCTTATGGGACATTGGAAGCAGGATCCACTAAGGAGTTCTTCAGGAGGTCTAA; AATCGCTGTGTTTGAGAAGATGTGGACATACATGAAGTCTGCAGAACCGTCTGTGTTTGTTCGGACCACA; GAGGAGGGCATGATCAGAGTGAGAAAGTCTAAAGGCAAATATGCCTACCTCCTGGAGTCCACCATGAATG; AGTACATTGAGCAACGCAAGCCCTGTGACACCATGAAAGTGGGAGGTAACTTGGATTCCAAAGGCTATGG; CATTGCAACACCCAAGGGGTCCGCCCTGAGAAATCCAGTAAACCTGGCAGTGTTAAAACTGAACGAGCAG; GGGCTTTTGGACAAATTGAAAAACAAATGGTGGTACGACAAGGGCGAGTGCGGCAGCGGGGGAGGTGACT; CCAAGGACAAGACCAGTGCTCTGAGCCTGAGCAATGTGGCAGGCGTGTTCTACATCCTGATTGGAGGGCT; GGGATTGGCCATGCTGGTTGCCTTAATCGAGTTCTGCTACAAATCCCGTAGCGAGTCGAAGCGGATGAAG; GGTTTCTGTTTGATTCCACAGCAATCCATCAATGAAGCCATACGGACATCGACCCTCCCCAGGAACAGCG; GGGCAGGAGCCAGCGGAGGAAGTGGCAGTGGAGAGAATGGCAGAGTGGTCAGCCAGGACTTCCCCAAGTC; CATGCAATCCATTCCCTGCATGAGCCACAGTTCAGGGATGCCCTTGGGAGCCACAGGATTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR211142 protein sequence; Red=Cloning site Green=Tags(s); ; MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEM; TYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHY; KWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLN; AILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDARDHTR; VDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLT; GNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYV; MLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAV; APLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRF; SPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSY; TANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTT; EEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQ; GLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMK; GFCLIPQQSINEAIRTSTLPRNSGAGASGGSGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008165
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008165.4
• RefSeq Size: 5755 bp
• RefSeq ORF: 2724 bp
• Locus ID: 14799
• Cytogenetics: 11 34.51 cM
• MW: 101.6 kDa
• Summary: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.[UniProtKB/Swiss-Prot Function]