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Ercc4 (BC026792) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211135
Ercc4 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $829.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ercc4 or BC026792"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ercc4
Synonyms AI606920; Xpf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211135 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCCGCTGTTGGAGTACGAGAGGCAGCAGGTGCTGGAACTGCTCGACAGCGACGGGCTGGTGGTGT
GTGCCCGCGGACTCGGCACCGACCGGCTCCTCTACCATTTCCTGCGGCTTCACTGCCACCCGGCCTGCCT
GGTGCTGGTGCTCAACACGCAGCCGGCCGAGGAGGAATATTTTATCAATCAGTTGAAGATAGAAGGAGTT
GAACATCTCCCTCGACGAGTGACAAATGAAATCGCAAGTAACAGTCGCTATGAAGTCTACACGCAGGGTG
GTATTATATTTGCAACAAGCCGAATACTCGTGGTTGATTTCTTGACAGGTAGAATACCTTCAGATTTAAT
AACTGGCATCCTCGTGTACAGGGCACACAGAATCATTGAGTCCTGCCAGGAAGCCTTCATCCTGCGCCTC
TTCCGCCAGAAAAACAAGCGCGGGTTCATCAAAGCTTTCACCGACAACGCTGTTGCCTTTGACACTGGCT
TTTGTCACGTGGAAAGAGTGATGCGGAACCTTTTTGTGAGGAAGCTCTACCTGTGGCCAAGGTTCCATGT
AGCAGTCAACTCATTCTTGGAACAACACAAGCCTGAAGTTGTAGAGATTCACGTGTCCATGACTCCCGCC
ATGCTTGCCATTCAGACGGCCATACTAGACATCTTAAATGCATGTCTGAAAGAACTGAAATGTCACAACC
CGTCACTTGAAGTAGAAGATTTGTCACTAGAAAACGCTCTTGGGAAGCCATTCGACAAGACAATCCGCCA
TTACTTGGACCCTTTGTGGCATCAGCTTGGAGCCAAGACTAAATCCTTGGTGCAGGATTTAAAGATACTA
AGAACCTTACTGCAGTATCTCTCTCAGTATGATTGTGTTACATTTCTGAATCTTCTGGAATCTCTGAGAG
CAACAGAGAAGGTGTTTGGTCAGAATTCAGGTTGGCTTTTCCTGGATGCTAGCACCTCCATGTTTGTGAA
TGCTCGTGCCCGAGTTTACCGAGTTCCAGATGTCAAACTGAACAAAAAGGCCAAAACATCAGAAAAGACG
TCGAGTCCAGAAGTTCAAGAAACAAAAAAAGAACTGGTTCTAGAGAGCAATCCAAAGTGGGAGGCGCTGA
CTGACGTCCTGAAAGAAATCGAAGCAGAAAATAAGGAGAGCGAAGCCCTTGGTGGCCCAGGCCGGGTGCT
GATCTGTGCAAGCGATGACCGCACGTGCTGCCAGCTGAGAGACTACCTGTCCGCCGGAGCAGAAACCTTC
CTGCTGCGACTTTATAGGAAAACTTTTGAGAAGGATGGCAAAGCTGAAGAAGTGTGGGTAAATGTTAGAA
AGGGGGACGGCCCGAAGAGAACTACAAAATCTGACAAAAGACCAAAAGCCGCTCCAAACAAGGAGCGTGC
TTCCGCCAAAAGGGGGGCCCCTCTCAAAAGGAAGAAGCAAGAGCTGACTCTGACGCAAGTTTTGGGGTCC
GCTGAGGAGCCGCCGGAGGACAAGGCTTTGGAGGAAGACCTGTGCAGACAGACCAGTAGTAGCCCAGAAG
GCTGCGGGGTAGAGATTAAGCGTGAATCATTTGATTTAAATGTGTCGTCTGATGCGGCCTATGGCATCCT
GAAAGAGCCCCTGACCATCATCCATCCGCTTCTAGGGTGCAGCGACCCCTATGCCCTGACGCGGGTACTG
CACGAGGTGGAGCCCAGATACGTGGTTCTGTATGATGCAGAGCTCACCTTTGTTCGCCAACTGGAGATCT
ACAGGGCTAGCAGGCCTGGGAAACCTCTGAGGGTTTACTTTCTTATATACGGAGGCTCCACGGAAGAGCA
GCGCTACCTGACTGCTCTGCGGAAAGAAAAGGAAGCCTTTGAAAAGCTCATAAGAGAAAAAGCTAGCATG
GTTGTCCCCGAAGAACGGGAAGGCAGAGATGAGACCAACCTGGACCTAGCAAGAGGCTCAGCAGCCCTGG
ATGCTCCCACGGACACTCGGAAAGCCGGTGGCCAGGAGCAGAATGGCACCCAGTCCAGCATCGTGGTGGA
CATGCGTGAGTTTCGGAGCGAGCTCCCATCTCTGATCCATCGGCGGGGTATTGACATAGAGCCAGTGACT
CTGGAGGTGGGTGATTACATCCTGACACCTGAGCTGTGTGTGGAGCGCAAAAGTGTGAGCGACCTCATTG
GCTCGCTGCACAGTGGCCGCCTGTACAGCCAGTGCCTAGCCATGTCTCGCTACTACCGGAGGCCTGTGCT
GCTCATTGAGTTTGACCCGAGCAAGCCCTTCTCCCTGGCGCCACGAGGTGCCTTCTTCCAGGAGATGAGC
AGCAGCGATGTGAGCTCCAAGCTTACGCTCCTCACCTTGCACTTCCCGCGTTTGCGGCTGCTCTGGTGCC
CCTCACCCCACGCCACAGCCGAGCTGTTTGAGGAGCTGAAGCAGAACAAGCCACAGCCTGATGCGGCCAC
AGCCATGGCCATCACCGCAGACTCAGAGACACTGCCTGAGTCGGACAGATATAACCCCGGCCCCCAAGAC
TTTGTGCTGAAGATGCCTGGGGTCAACGCCAAGAACTGTCGCTCCCTGATGAACCAAGTGAAGAACATCG
CAGAGCTGGCCACCCTGTCCCTGGAACGACTCACAACCATCCTGGGGCACTCGGGAAATGCCAAGCAGCT
GCACGACTTCCTGCACACTGCCTATGCAGACTTGGTGTCCAAAGGCAGAGTGAGAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211135 protein sequence
Red=Cloning site Green=Tags(s)

MAPLLEYERQQVLELLDSDGLVVCARGLGTDRLLYHFLRLHCHPACLVLVLNTQPAEEEYFINQLKIEGV
EHLPRRVTNEIASNSRYEVYTQGGIIFATSRILVVDFLTGRIPSDLITGILVYRAHRIIESCQEAFILRL
FRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPA
MLAIQTAILDILNACLKELKCHNPSLEVEDLSLENALGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKIL
RTLLQYLSQYDCVTFLNLLESLRATEKVFGQNSGWLFLDASTSMFVNARARVYRVPDVKLNKKAKTSEKT
SSPEVQETKKELVLESNPKWEALTDVLKEIEAENKESEALGGPGRVLICASDDRTCCQLRDYLSAGAETF
LLRLYRKTFEKDGKAEEVWVNVRKGDGPKRTTKSDKRPKAAPNKERASAKRGAPLKRKKQELTLTQVLGS
AEEPPEDKALEEDLCRQTSSSPEGCGVEIKRESFDLNVSSDAAYGILKEPLTIIHPLLGCSDPYALTRVL
HEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASM
VVPEEREGRDETNLDLARGSAALDAPTDTRKAGGQEQNGTQSSIVVDMREFRSELPSLIHRRGIDIEPVT
LEVGDYILTPELCVERKSVSDLIGSLHSGRLYSQCLAMSRYYRRPVLLIEFDPSKPFSLAPRGAFFQEMS
SSDVSSKLTLLTLHFPRLRLLWCPSPHATAELFEELKQNKPQPDAATAMAITADSETLPESDRYNPGPQD
FVLKMPGVNAKNCRSLMNQVKNIAELATLSLERLTTILGHSGNAKQLHDFLHTAYADLVSKGRVRK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC026792
ORF Size 2718 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC026792, AAH26792
RefSeq Size 3235 bp
RefSeq ORF 2720 bp
Locus ID 50505
Cytogenetics 16 A1
MW 102.5 kDa
Summary Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC026792" in other vectors (4)
SKU Description Size Price
MC220780 Ercc4 (untagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522, (10ug) 10 ug
$913.00
MG211135 Ercc4 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 10 ug
$1,029.00
MR211135L3 Lenti ORF clone of Ercc4 (Myc-DDK-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 10 ug
$1,129.00
MR211135L4 Lenti ORF clone of Ercc4 (mGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522 10 ug
$1,129.00

Plasmid Map for MR211135

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211135
Circular map for MR211135

file-alt Citations

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