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Mcm2 (NM_008564) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211123
Mcm2 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $828.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mcm2 or NM_008564"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcm2
Synonyms AA959861; AW476101; BM28; CDCL1; Mcmd2; mKIAA0030
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211123 representing NM_008564
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGTCTTCTGAGTCTCTCTCAGCATCTAGCCCTGCCCGTCAGCGGCGCCGGATCAGTGATCCCC
TCACCTCCAGCCCAGGCCGCAGCTCCAGACGTGCTGACGCCCTGACCTCCAGCCCTGGCAGAGACCTCCC
CCCATTTGAAGATGAGTCTGAGGGGCTGCTGGGCACAGAGGGGCCCATGGAGGAAGAAGAGGATGGAGAG
GAACTCATTGGTGATGGCATGGAGAGAGACTACCGTCCCATTCCGGAGCTCGATGTCTACGAGGCCGAGG
GATTGGCCCTGGATGATGAAGATGTGGAGGAGCTGACAGCCAGTCAGAGAGAGGCAGCTGAGCGGACCAT
GAGGCAGCGGGACCGTGAGGCTGGCAGAGGCCTGGGACGCATGCGCCGGGGGCTGCTCTATGACAGCAGC
GAGGAAGATGAGGAGCGGCCTGCCCGTAAGCGCCGCCACGTAGAACGCGCCACAGAGGATGGCGAGGAGG
ATGAAGAGATGATCGAGAGTATTGAGAATCTGGAGGACCTCAAGGGCCACTCGGTGCGCGAGTGGGTGAG
CATGGCAGGGCCCAGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCGCACCCACGTGGACAGCCAT
GGCCACAACGTCTTCAAGGAGCGCATCAGTGATATGTGCAAAGAGAACCGTGAGAGTTTGGTGGTAAATT
ATGAAGACCTGGCAGCCCGGGAGCACGTGTTGGCATACTTCCTGCCGGAAGCACCGGCTGAGTTGCTGCA
GATCTTTGACGAGGCTGCCCTGGAGGTCGTGTTGGCCATGTACCCTAAATATGACCGTATCACCAACCAC
ATCCATGTGCGCATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGTTCACTGAGGCAGTTGCACCTGAACC
AGCTGATCCGTACCAGTGGCGTGGTGACCAGCTGCACCGGAGTCCTGCCCCAGCTCAGCATGGTCAAGTA
CAACTGTAGCAAGTGCAACTTTGTACTGGGGCCTTTCTGCCAGTCTCAGAATCAGGAGGTGAAGCCTGGC
TCCTGCCCTGAGTGCCAGTCTGCTGGGCCCTTTGAGATCAACATGGAGGAGACCATCTATCAGAACTACC
AACGTATCCGCATCCAGGAGAGTCCCGGCAAGGTGGCGGCTGGCCGACTGCCCCGTTCCAAGGATGCCAT
TCTCCTCGCTGATCTGGTGGACAGCTGCAAGCCAGGGGACGAGATAGAGCTGACCGGCATTTACCATAAT
AACTATGACGGCTCGCTTAACACCGCCAACGGCTTTCCAGTCTTTGCCACTATTATCTTGGCCAACCATG
TTGCCAAGAAGGACAACAAAGTAGCTGTGGGGGAGCTCACCGATGAGGACGTGAAGATGATCACCGGTCT
CTCCAAGGATCAGCAAATTGGAGAGAAGATCTTTGCCAGCATTGCACCCTCCATCTATGGGCATGAAGAC
ATCAAGAGAGGCCTGGCTCTGGCCCTGTTTGGAGGGGAGCCCAAGAACCCAGGTGGAAAGCACAAGGTTC
GAGGTGACATTAATGTGCTCTTGTGTGGGGACCCTGGCACAGCAAAGTCCCAATTCCTCAAATACATCGA
GAAAGTGTCTAGCCGTGCCATCTTCACCACTGGCCAGGGTGCGTCAGCAGTGGGTCTCACCGCGTACGTT
CAGCGGCATCCCGTCAGCAGAGAGTGGACCTTAGAGGCGGGAGCCCTGGTTCTGGCTGACCGGGGGGTGT
GTCTCATTGACGAGTTTGACAAGATGAATGACCAGGACAGGACCAGCATCCACGAGGCCATGGAACAGCA
AAGCATCTCCATCTCCAAGGCTGGCATCGTTACCTCGCTGCAAGCCCGCTGCACTGTCATAGCTGCTGCC
AACCCCATAGGAGGCCGCTACGACCCTTCACTGACCTTCTCAGAGAATGTAGACCTCACAGAGCCCATCA
TTTCCCGCTTTGATGTCCTGTGTGTGGTGAGGGACACTGTTGATCCAGTTCAGGATGAGATGCTGGCCCG
CTTTGTGGTTGGCAGCCACGTCAGACACCACCCCAGTAACAAGAAGGATGAAGGGTTGACTAATGGTGGC
ACCTTGGAGCCAGCCATGCCCAACACATATGGCGTGGAGCCCCTGCCTCAGGAGGTGCTGAAGAAGTATA
TCATCTATGCCAAGGAGAGGGTCCGCCCGAAGCTCAACCAGATGGACCAGGATAAAGTGGCCAGGATGTA
CAGTGACCTGAGGAAGGAGTCCATGGCAACGGGCAGCATTCCCATCACGGTGCGCCACATCGAGTCCATG
ATCCGCATGGCCGAGGCCCATGCCCGCATGCACCTGCGGGACTACGTGATGGAAGACGATGTCAACATGG
CCATCCGAGTGATGATGGAGAGCTTCATTGACACCCAGAAGTTCAGCGTCATGCGGAGTATGCGCAAGAC
TTTTGCCCGGTATCTCTCCTTCCGGCGAGATAACAATGATCTGCTGCTCTTCATACTGAAGCAGTTGGTG
GCTGAGCAGGTGACATATCAACGCAACCGCTTTGGGGCCCAGCAGGACACCATTGAAATACCTGAGAAGG
ATCTGATGGACAAGGCCAGGCAGATCAATATTCACAACCTCTCTGCCTTCTACGACAGCGACCTCTTCAA
ATTCAACAAGTTCAGCCGTGACCTGAAACGCAAACTGATCCTACAGCAGTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211123 representing NM_008564
Red=Cloning site Green=Tags(s)

MAESSESLSASSPARQRRRISDPLTSSPGRSSRRADALTSSPGRDLPPFEDESEGLLGTEGPMEEEEDGE
ELIGDGMERDYRPIPELDVYEAEGLALDDEDVEELTASQREAAERTMRQRDREAGRGLGRMRRGLLYDSS
EEDEERPARKRRHVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSH
GHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNH
IHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCSKCNFVLGPFCQSQNQEVKPG
SCPECQSAGPFEINMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
NYDGSLNTANGFPVFATIILANHVAKKDNKVAVGELTDEDVKMITGLSKDQQIGEKIFASIAPSIYGHED
IKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYV
QRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAA
NPIGGRYDPSLTFSENVDLTEPIISRFDVLCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKKDEGLTNGG
TLEPAMPNTYGVEPLPQEVLKKYIIYAKERVRPKLNQMDQDKVARMYSDLRKESMATGSIPITVRHIESM
IRMAEAHARMHLRDYVMEDDVNMAIRVMMESFIDTQKFSVMRSMRKTFARYLSFRRDNNDLLLFILKQLV
AEQVTYQRNRFGAQQDTIEIPEKDLMDKARQINIHNLSAFYDSDLFKFNKFSRDLKRKLILQQF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008564
ORF Size 2712 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008564.2, NP_032590.2
RefSeq Size 3381 bp
RefSeq ORF 2715 bp
Locus ID 17216
UniProt ID P97310
Cytogenetics 6 D1
MW 102.5 kDa
Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.UniProtKB/Swiss-Prot Function
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Documents
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"NM_008564" in other vectors (4)
SKU Description Size Price
MC222517 Mcm2 (untagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2), (10ug) 10 ug
$911.00
MG211123 Mcm2 (tGFP-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S 10 ug
$1,028.00
MR211123L3 Lenti ORF clone of Mcm2 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2) 10 ug
$1,128.00
MR211123L4 Lenti ORF clone of Mcm2 (mGFP-tagged) - Mouse minichromosome maintenance deficient 2 mitotin (S. cerevisiae) (Mcm2) 10 ug
$1,128.00

Plasmid Map for MR211123

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211123
Circular map for MR211123

file-alt Citations

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