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BC055324 (NM_201364) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211119
BC055324 (Myc-DDK-tagged) - Mouse cDNA sequence BC055324 (BC055324)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $827.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol BC055324
Synonyms MGC65590
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211119 representing NM_201364
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGCAGGACGATGCGGAGGTGGCGAGCGGGGTCGTGCTGGAGGAACTCAGCAGCTGGTCGGAGGAGA
TGTGCCGTCGGGAGCTGCCTTCGGTGCTGCCCCGGCTCCTCTCTATGTATCAATGTTCTGAAAGTTGGAT
TGAACATATCCGAATTTTAAAAATCATTGTTGAAATGTTTTTGCCTCATATGAACCACCTGACACTGGAA
GAAACTTTGTTTTCCCAAGTTTTACCAAAGAGTATAAAATTGTTTGATGGCATGATATGTGAATTAACCA
GTGAAGCCAGAGAACTCTCCAGCCAAAATTTAGAAATCCAGGTCACCATAAGGAACATTTTACAAGCAAT
GGTGCAGGTCATAGGAGGTTTTACAGGATGTGTCCGTCATGTCTGTGCCACACAGAAATCTGTCTTTCTA
GGAAGTATTCAGAGTCTTCCCTCATTCATCCTGCATATAATTAAAAGTGCATTTGTGCACTGTAAGAACA
GCGAATGTGTGTATTCTGGGCGCTTACACCTAGTGTCAGACCTTCTCCAGGTTCTGTTCAAGGAGGCCTA
TTCTCTCCAGAAGCAGCTGATGGGACTGCTGGACACGGTTTGCCTGGACCCTTCAGTAGATGAGAATAAC
GCTTTGATAATGGTAGGAGTTATTCATTCATTATTGGATATCTGCTCTGTTATCTCTGGTATGGACCAAG
CATTTCATGCCAATACTTGGAAGTTTATTATTAAGCAGAGCCTCAAACACCACTCGGTTATAAAGAGCCA
GTTGAGACACAAAGAGATCATTTCTAGCTTGTGTGAAGACATTCTTTTCTCCTTTCATTCTTGTTTACAG
TTAGCTGAGCAGATAACACAGCCAGCTGCACAGGGTAATGCTGACTACAGGTTATTTCAAAAAACACTGA
AATTGTGTCGTTTCTTTGCTAACTCCCTTTTGCACTACACTAAGGAATGTCTTCCTTTCCTGTCTGATTC
TTGCTGTACTCTGCACCAGCTTTATCTGCAGATACACAGCAAGTTTCTAAGCCTCTGTGCTGCCAAGACT
TCCAAAGCCCAGCAGGAGGAAATAGCAAGCACTTTCCTTGTGCTACTGGACCCACTCATCAGTCAGCTGC
TCAAATCCCAGCCTTTTGTGCAGGCAGTTTTGGCGAGTAAACTTGCCCTGCCCTGTGAGCTGCAGCTCCC
ACAAGTCCTACTCCTGGTTGTCGCCATGGATAAACTGCCCTCTCAGCCACAGGACGTGCAGACCCTGTGG
AGCACAGAGGATATGACTAGGATGTCCATACTGAAAGGAATTTTCTACAATTTTGGCCAGTGTTCTGGTG
AACTCTCTCTGCCTACTCATTTACAAGGAACGAAGGGTAAAGGACAAGCTGAGGTGCCTGTCACGTTGTA
TCAGCATGTTTGTGTTCATCTGTGTGCATTTGTGGCTTCTTTTCATTCTTCATTGTTTCCTCGGCTGGAT
GCTGCTCTATTGAATGCTGTGCTTAGTACCAATATGAGTACCTCTTTGTTAGCGATGGATGTGTGGTGCT
TCCTTGCTCGGTATGGGACTGCTAAACTTGGTGCACACCATGTCACCCTAGTGGCTCATCTGGTTAAATC
TTGCCCTGGAAAATGTGTTCAGCTTACCAACCTCTCCATACTGTTGAAGCGCCTCCTTTTCTTCATGGCT
GCACCCCATCAGGTGCAATTCATCCAGAAATTTTCTCCAAAAGAAGCAGACAACTTGCATTTGTGGCAGT
ATATTTCCCTCCAGGCGTTCGATGCTGATCTCAGGAAGCCGGTTGCATGTGAATTGGTCAGAGTGTGCAG
AGCACAGTGCAGGAAGTGGTTGAGCAGCACTCGGACTCTGGCAGAGCTTGACTCTCTGAACACAGCACTT
TCTGTTGTACTTACTGTCTGCAATTCTGCTGGAGAAGCTTTGGATAGTAGACAGCTAACTGCAGTTACTG
AAGTTTTGGGTGAGCTTTGGACTTTCATAAACGTTGAACAAATTATCAGTCAGCCCTATGTTCAACAGGC
ATTTAGCCTTTTACTTCAACTGCTGGCGTTTTTCATCCAAACTGTAGATCTGCAGCTGATAAGTCAGGTT
GTAAATGTCCTGACTTCGGTGATTAAACTAGAGCCTCCTGACCACGTCTCTTTGGCAGTGCTGGATTTTA
TATCGTCTCTGGGCAAACTTTATATATCTCAAACTATCCGGGACAAGGTGCTGCCCAGCTTGTCTTGTAT
TCTTACTTCACTGATCGTCAACAAGAATTGGTTGCTAGAGCAACATACTTTGGAGGCATTTACTCAGTTT
GCTGAGGGAACAAAGCATGAAGAGATAGTTCCACAGTGTCTCGGTTCTGAAGAAATCAAGAACAAAGTTG
TATCCTTTTTGGAGAAGACAGAATCTGTAGATGAAGCTGAGGTTGCCACAGTGGACAATGTGAAGCAGGA
GAAAGGCACTTTCTGGGAACCTGCTGCTAAGGTGACTGTAGAAGAAGTGAAGACGTCAGCGTTTCAGCCT
CACACAAAAAGAGCCCGTCGGGTGTTGCCCTTTGAAGAAGAATACAGATCAGTATTTAAAGCTGCAGCAA
GGGCTTTGGAGACAACTGAGTTTTTACTCAAACACTCTCTTGCTCCAGCATGGCTGCTGCCAGAACTGGA
GGCGCTGCAAGGAAGGATCGAGAAGCTAAAACGCTGTGTGCTTACAGGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211119 representing NM_201364
Red=Cloning site Green=Tags(s)

MSQDDAEVASGVVLEELSSWSEEMCRRELPSVLPRLLSMYQCSESWIEHIRILKIIVEMFLPHMNHLTLE
ETLFSQVLPKSIKLFDGMICELTSEARELSSQNLEIQVTIRNILQAMVQVIGGFTGCVRHVCATQKSVFL
GSIQSLPSFILHIIKSAFVHCKNSECVYSGRLHLVSDLLQVLFKEAYSLQKQLMGLLDTVCLDPSVDENN
ALIMVGVIHSLLDICSVISGMDQAFHANTWKFIIKQSLKHHSVIKSQLRHKEIISSLCEDILFSFHSCLQ
LAEQITQPAAQGNADYRLFQKTLKLCRFFANSLLHYTKECLPFLSDSCCTLHQLYLQIHSKFLSLCAAKT
SKAQQEEIASTFLVLLDPLISQLLKSQPFVQAVLASKLALPCELQLPQVLLLVVAMDKLPSQPQDVQTLW
STEDMTRMSILKGIFYNFGQCSGELSLPTHLQGTKGKGQAEVPVTLYQHVCVHLCAFVASFHSSLFPRLD
AALLNAVLSTNMSTSLLAMDVWCFLARYGTAKLGAHHVTLVAHLVKSCPGKCVQLTNLSILLKRLLFFMA
APHQVQFIQKFSPKEADNLHLWQYISLQAFDADLRKPVACELVRVCRAQCRKWLSSTRTLAELDSLNTAL
SVVLTVCNSAGEALDSRQLTAVTEVLGELWTFINVEQIISQPYVQQAFSLLLQLLAFFIQTVDLQLISQV
VNVLTSVIKLEPPDHVSLAVLDFISSLGKLYISQTIRDKVLPSLSCILTSLIVNKNWLLEQHTLEAFTQF
AEGTKHEEIVPQCLGSEEIKNKVVSFLEKTESVDEAEVATVDNVKQEKGTFWEPAAKVTVEEVKTSAFQP
HTKRARRVLPFEEEYRSVFKAAARALETTEFLLKHSLAPAWLLPELEALQGRIEKLKRCVLTG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201364
ORF Size 2709 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201364.1, NP_958752.1
RefSeq Size 2930 bp
RefSeq ORF 2712 bp
Locus ID 381306
UniProt ID Q3TQQ9
Cytogenetics 1 H2.2
MW 101.7 kDa
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Documents
Resources
"NM_201364" in other vectors (4)
SKU Description Size Price
MC222499 BC055324 (untagged) - Mouse cDNA sequence BC055324 (BC055324), (10ug) 10 ug
$910.00
MG211119 BC055324 (tGFP-tagged) - Mouse cDNA sequence BC055324 (BC055324) 10 ug
$1,027.00
MR211119L3 Lenti ORF clone of BC055324 (Myc-DDK-tagged) - Mouse cDNA sequence BC055324 (BC055324) 10 ug
$1,127.00
MR211119L4 Lenti ORF clone of BC055324 (mGFP-tagged) - Mouse cDNA sequence BC055324 (BC055324) 10 ug
$1,127.00

Plasmid Map for MR211119

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211119
Circular map for MR211119

file-alt Citations

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