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Tiam1 (BC065126) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211096
Tiam1 (Myc-DDK-tagged) - Mouse T-cell lymphoma invasion and metastasis 1 (cDNA clone MGC:86168 IMAGE:30534934)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $824.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tiam1
Synonyms AI847750; D16Ium10; D16Ium10e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211096 representing BC065126
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAACGCAGAAAGCCAAAATGTAGACCACGAGTTTTATGGAGAAAAGCATGCCAGCCTGGGGCGGA
AGCACACTTCACGCTCCCTGCGGCTGTCACACAAGACCCGGAGGACACGGCATGCTTCCTCCGGGAAGGC
GATCCACAGGAACTCTGAAGTGAGCACCCGGTCCAGCAGCACGCCCAGCATCCCCCAGTCCCTGGCTGAA
AATGGCCTGGAGCCCTTCTCCCAAGAAGGCGCCCTAGACGACTTCGGGGACCCCATCTGGGTGGACCGAG
TAGATATGGGCTTGAGACCCGTATCTTACACCGATTCTTCCGTCACTCCCAGCGTAGATGGCAGCATCGT
CCTCACTGCAGCCTCTGTGCAGAGCATGCCAGACTCAGAGGAGAGCCGGCTTTACGGGGATGACGCTACG
TACTTGGCTGAGGGAGGCAGGAGGCAGTGTCCCTATACATCCAATGGGCCCACATTCATGGAGACAGCGA
GCTTTAAGAAGAAGCGCTCCAAATCTGCAGACATCTGGCGGGAGGACAGCCTGGAATTCTCACTCTCAGA
TCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTGGGTTCCGCGGAAGAGAAGGATTGCGAGGAG
GCTCGGGGGATGGAAACAGAGGCGAGTCCCCGGCAGCTCAGCACCTGTCAGCGAGCCAACTCCCTGGGTG
ACTTGTATGCTCAGAAAAACTCTGGGGTGAAGGCTAATGGAGGACCGAGGAACAGATTCTCAAGCTACTG
CCGGAATTTGGTGTCGGATATTCCCGATCTTGCAAAGCATAAGATGCCACCGGCTGCTGCCGAAGAGACT
CCTCCGTACAGTAATTACAACACGCTTCCCTGTAGGAAGTCGCACTGTCTTTCCGAGGGTGCCACCAACC
CACAAATTAGCCTTAGCAAGAGCATGCAAGGCAGAAGAGCAAAAACCACCCAGGATGTTAACACAGGCGA
GGGCAGCGAGTTTGCGGACAGTGGGATCGAAGGGGCCACCACCGACACGGACCTCTTGTCCAGGAGATCC
AATGCCACCAACTCAAGCTACTCGCCCCCCACAGGCCGAGCCTTTGTGGGCAGCGACAGCGGCAGCAGTT
CAACAGGGGATGCTGCGCGCCAGGGGGTGTACGAGAACTTCAGGCGGGAACTGGAGATGAGTACCACCAA
CAGTGAGAGCCTGGAGGAGGCTGGCTCTGCGCACAGCGATGAGCAGAGCAGCGGAACCCTCAGTTCCCCG
GGCCAGTCGGACATCCTGCTAACGGCTGCGCAGGGCACTGTGCGGAAGGCTGGAGCGCTGGCAGTCAAGA
ACTTCCTGGTCCACAAGAAGAATAAGAAGGTGGAGTCGGCTACCCGGAGGAAGTGGAAGCACTACTGGGT
GTCCCTGAAAGGTTGCACGCTCTTTTTCTATGAGACTGATGGCAGGTCTGGGATAGACCACAACAGTGTC
CCCAAGCACGCCGTCTGGGTGGAGAACAGCATCGTGCAGGCTGTGCCTGAGCACCCCAAGAAAGACTTCG
TCTTCTGCCTCAGCAACTCCCTGGGTGACGCCTTCCTCTTCCAGACCACGAGCCAGACAGAGCTGGAGAA
CTGGATCACCGCCATCCACTCTGCCTGCGCAGCTGCCGTCGCAAGGCACCACCACAAGGAGGACACGCTC
CGCCTCCTCAAGTCGGAAATCAAGAAGCTGGAGCAGAAGATCGACATGGACGAGAAGATGAAGAAGATGG
GGGAGATGCAGCTGTCTTCCGTCACCGACTCGAAGAAGAAGAAGACCATCCTAGACCAGATCTTCGTTTG
GGAGCAGAACCTCGAACAGTTCCAGATGGACCTGTTCCGATTCCGCTGTTACCTCGCCAGCCTCCAGGGC
GGGGAGCTGCCAAACCCCAAAAGGCTGCTTGCTTTTGCGAGCCGGCCCACGAAGGTGGCCATGGGCCGCC
TCGGGATCTTCTCCGTGTCATCTTTTCATGCCCTGGTGGCAGCCCGGACTGGTGAGATCGGAGTGAGAAG
GCGTACTCAGGCCATGTCCAGGTCTGCAAGCAAGCGAAGGAGCAGAAAGGACTTTCTGAAGTCTGTGCAT
TCGATCCTGCGAGATAAACACAGAAGACAGCTCCTCAAAACGGAAAGCCTTCCGTCAGCCCAGCAGTATG
TCCCCTTTGGAGGCAAGAGATTGTGTGCGCTTAAAGGCGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGC
CCCAAGCAAGTCTCTTGGGAGGAGGAGGCGGCGACTGGCCCGAAACAGGTTTACCATCGATTCAGACGCC
ATCTCAGCCAGCAGCCCGGAGAAAGAGCCCCAGCAGCCCGCCGGTGGTGGGGACACTGACCGATGGGTAG
AGGAACAGTTCGATCTTGCTCAGTACGAGGAGCAGGATGACATCAAGGAGACAGACATCCTCAGTGACGA
TGACGAATTCTGTGAGTCCCTGAAGGGCGCCTCAGTGGACAGAGACCTTCAGGAGCAGCTTCAGGCTGCC
TCCATCAGTCAGCGGGCCCGAGGCCGGAGAACCCTCGATAGCCACGCCTCCCGCATGACACAGCTCAAGA
AGCAAGCGGCCCTCTCGGGCATCAACGGGGGCCTGGAGAGTGCGAGCGAGGAAGTCATTTGGGTCAGGCG
CGAAGACTTTGCCCCCTCCAGGAAACTGAACACGGAGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211096 representing BC065126
Red=Cloning site Green=Tags(s)

MGNAESQNVDHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKAIHRNSEVSTRSSSTPSIPQSLAE
NGLEPFSQEGALDDFGDPIWVDRVDMGLRPVSYTDSSVTPSVDGSIVLTAASVQSMPDSEESRLYGDDAT
YLAEGGRRQCPYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEE
ARGMETEASPRQLSTCQRANSLGDLYAQKNSGVKANGGPRNRFSSYCRNLVSDIPDLAKHKMPPAAAEET
PPYSNYNTLPCRKSHCLSEGATNPQISLSKSMQGRRAKTTQDVNTGEGSEFADSGIEGATTDTDLLSRRS
NATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSP
GQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYETDGRSGIDHNSV
PKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACAAAVARHHHKEDTL
RLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQG
GELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGEIGVRRRTQAMSRSASKRRSRKDFLKSVH
SILRDKHRRQLLKTESLPSAQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDA
ISASSPEKEPQQPAGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESLKGASVDRDLQEQLQAA
SISQRARGRRTLDSHASRMTQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC065126
ORF Size 2700 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC065126.1
RefSeq Size 5225 bp
RefSeq ORF 2702 bp
Locus ID 21844
Cytogenetics 16 51.5 cM
MW 191.5 kDa
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"BC065126" in other vectors (4)
SKU Description Size Price
MC220765 Tiam1 (untagged) - Mouse T-cell lymphoma invasion and metastasis 1 (cDNA clone MGC:86168 IMAGE:30534934), (10ug) 10 ug
$907.00
MG211096 Tiam1 (tGFP-tagged) - Mouse T-cell lymphoma invasion and metastasis 1 (cDNA clone MGC:86168 IMAGE:30534934) 10 ug
$789.00 $1,024.00
MR211096L3 Lenti ORF clone of Tiam1 (Myc-DDK-tagged) - Mouse T-cell lymphoma invasion and metastasis 1 (cDNA clone MGC:86168 IMAGE:30534934) 10 ug
$1,124.00
MR211096L4 Lenti ORF clone of Tiam1 (mGFP-tagged) - Mouse T-cell lymphoma invasion and metastasis 1 (cDNA clone MGC:86168 IMAGE:30534934) 10 ug
$1,124.00

Plasmid Map for MR211096

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211096
Circular map for MR211096

file-alt Citations

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