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Wwp2 (NM_025830) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211018
Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $797.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Wwp2
Synonyms 1300010O06Rik; AA690238; AIP2; AW554328
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211018 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTGCCAGCTCCAGCCGGGCAGGAGTGGCCCTGCCTTTTGAGAAGTCCCAGCTTACCCTGAAAG
TGGTGTCAGCAAAGCCCAAGGTGCACAACCGCCAGCCCAGAATCAACTCCTACGTGGAGGTGGCAGTGGA
TGGACTCCCCAGCGAGACCAAGAAGACGGGGAAGCGCATCGGGAGCTCTGAACTGCTCTGGAATGAAATC
ATCGTTCTGAATGTCACAGCCCAGAGTCATTTGGATCTGAAGGTCTGGAGCTGTCATACCTTGAGGAATG
AACTACTGGGCACTGCCTCTGTCAACCTCTCCAATGTCCTGAAGAACAATGGCGGCAAAATGGAGAACAC
ACAACTGACCCTGAACCTGCAGACAGAGAACAAAGGCAGTGTTGTCTCGGGAGGAGAGCTGACAATTTTC
CTGGATGGGCCAACTGTTGATCTGGGAAGTGTGCCTAATGGCAGTGCAGTGACAGACGGATCACAGCCAC
CTTCAAGAGAATCCAGTGGGACTGCTATAGCTCCAGAGACCCGGCACCAGCCCCCCAGTACAAACTGCTT
TGGTGGCAGATCCCGGACGCACAGACACTCAGGTGGCTCAGCCAGGACAGCCACAGCAGCCAGTGAACAA
AGCCCTGGCGCTAGGAACCGCCACCGCCAGCCTGTGAAGAACTCTAGCAGCAGTGGCTTAGCCAATGGCA
CAGTGAATGAGGAACCTACTCCAGCCAGTGAACCTGAAGAATCGTCGGTTGTTGGTGTAACATCCCTGCC
TGCAGCAGCCTTGAGTGTGTCCTCAAATCCCAACACAACATCTCTCCCTGCACAGTCCACACCAGCAGAG
GGAGAGGAGGCCAGCACTTCCGGGACACAGCAGCTCCCTGCTGCCGCCCAGGCCCCTGATGCTCTTCCTG
CTGGATGGGAACAGAGAGAGCTGCCCAATGGGCGTGTCTATTATGTTGATCACAACACCAAGACCACCAC
CTGGGAGCGGCCTCTTCCTCCAGGGTGGGAAAAGCGCACGGACCCCCGAGGGAGGTTTTACTACGTGGAC
CACAACACCCGGACAACCACCTGGCAGCGCCCAACTGCTGAGTACGTGCGCAACTATGAGCAGTGGCAGT
CCCAGCGGAACCAGCTGCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTCTACCAGTCTTCGAGTGC
TTCGACTGACCATGATCCCTTGGGCCCCCTCCCACCTGGCTGGGAGAAGAGGCAGGACAATGGACGGGTG
TATTATGTCAACCACAACACTCGCACTACCCAGTGGGAGGACCCTCGGACCCAGGGGATGATACAGGAGC
CAGCCCTGCCCCCAGGGTGGGAGATGAAATACACCAGCGAGGGCGTGCGGTACTTTGTGGACCACAATAC
CCGCACCACTACCTTTAAGGATCCTCGCCCAGGGTTCGAGTCAGGGACAAAGCAAGGCTCACCTGGTGCC
TATGACCGAAGTTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGCCACTCAAATGCTCTACCCAGCC
ATGTGAAGATCAGCGTTTCCAGGCAGACACTCTTTGAGGATTCTTTCCAACAGATTATGAACATGAAACC
TTACGACCTGCGCCGCCGGCTCTACATCATCATGCGTGGTGAGGAGGGCCTGGACTACGGCGGCATCGCC
AGAGAGTGGTTTTTCCTCCTGTCCCATGAGGTGCTCAACCCTATGTACTGTTTGTTTGAATATGCTGGGA
AGAACAATTACTGCCTGCAGATCAACCCGGCCTCTTCCATCAACCCTGACCACCTCACCTACTTCCGCTT
TATCGGCAGATTCATCGCCATGGCTCTGTACCATGGGAAGTTCATCGACACAGGCTTCACTCTCCCTTTC
TACAAGCGGATGCTCAACAAGAGACCGACTCTGAAGGACCTGGAGTCTATTGACCCTGAGTTTTACAACT
CCATTGTCTGGATCAAAGAGAACAACCTGGAAGAGTGTGGTCTGGAGCTGTTTTTCATCCAGGACATGGA
GATTCTGGGCAAGGTGACAACCCATGAACTGAAGGAGGGCGGTGAGAACATCCGAGTTACCGAGGAGAAC
AAGGAGGAGTATATCATGCTGCTGACTGACTGGCGATTCACCCGAGGCGTGGAAGAGCAGACCAAAGCTT
TCCTGGATGGCTTCAATGAGGTTGCCCCTCTGGAGTGGTTGAGATATTTTGATGAGAAAGAGCTGGAGCT
CATGCTCTGCGGCATGCAGGAGATAGACATGAGCGACTGGCAGAAGAACGCCATCTATCGGCACTACACC
AAGAGCAGCAAGCAGATCCAGTGGTTCTGGCAGGTTGTCAAGGAGATGGACAATGAGAAGAGGATCCGGC
TACTGCAGTTTGTCACGGGAACCTGCCGTCTGCCTGTTGGGGGATTTGCTGAGCTCATCGGGAGCAATGG
CCCGCAGAAGTTCTGCATCGACAGAGTTGGCAAGGAAACCTGGCTGCCCAGGAGCCATACGTGCTTCAAC
CGTCTGGACCTGCCTCCCTATAAGAGCTACGAGCAGCTGAAAGAGAAGCTGCTGTACGCCATCGAGGAGA
CTGAGGGGTTCGGACAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR211018 protein sequence
Red=Cloning site Green=Tags(s)

MASASSSRAGVALPFEKSQLTLKVVSAKPKVHNRQPRINSYVEVAVDGLPSETKKTGKRIGSSELLWNEI
IVLNVTAQSHLDLKVWSCHTLRNELLGTASVNLSNVLKNNGGKMENTQLTLNLQTENKGSVVSGGELTIF
LDGPTVDLGSVPNGSAVTDGSQPPSRESSGTAIAPETRHQPPSTNCFGGRSRTHRHSGGSARTATAASEQ
SPGARNRHRQPVKNSSSSGLANGTVNEEPTPASEPEESSVVGVTSLPAAALSVSSNPNTTSLPAQSTPAE
GEEASTSGTQQLPAAAQAPDALPAGWEQRELPNGRVYYVDHNTKTTTWERPLPPGWEKRTDPRGRFYYVD
HNTRTTTWQRPTAEYVRNYEQWQSQRNQLQGAMQHFSQRFLYQSSSASTDHDPLGPLPPGWEKRQDNGRV
YYVNHNTRTTQWEDPRTQGMIQEPALPPGWEMKYTSEGVRYFVDHNTRTTTFKDPRPGFESGTKQGSPGA
YDRSFRWKYHQFRFLCHSNALPSHVKISVSRQTLFEDSFQQIMNMKPYDLRRRLYIIMRGEEGLDYGGIA
REWFFLLSHEVLNPMYCLFEYAGKNNYCLQINPASSINPDHLTYFRFIGRFIAMALYHGKFIDTGFTLPF
YKRMLNKRPTLKDLESIDPEFYNSIVWIKENNLEECGLELFFIQDMEILGKVTTHELKEGGENIRVTEEN
KEEYIMLLTDWRFTRGVEEQTKAFLDGFNEVAPLEWLRYFDEKELELMLCGMQEIDMSDWQKNAIYRHYT
KSSKQIQWFWQVVKEMDNEKRIRLLQFVTGTCRLPVGGFAELIGSNGPQKFCIDRVGKETWLPRSHTCFN
RLDLPPYKSYEQLKEKLLYAIEETEGFGQE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025830
ORF Size 2610 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025830.4
RefSeq Size 4319 bp
RefSeq ORF 2613 bp
Locus ID 66894
UniProt ID Q9DBH0
Cytogenetics 8 D3
MW 98.8 kDa
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025830" in other vectors (4)
SKU Description Size Price
MC202685 Wwp2 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2), (10ug) 10 ug
$797.00
MG211018 Wwp2 (tGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$997.00
MR211018L3 Lenti ORF clone of Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$1,097.00
MR211018L4 Lenti ORF clone of Wwp2 (mGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$1,097.00

Plasmid Map for MR211018

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211018
Circular map for MR211018

file-alt Citations

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