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Wwp2 (NM_025830) Mouse Untagged Clone

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SKU
MC202685
Wwp2 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $797.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Wwp2
Synonyms 1300010O06Rik; AA690238; AIP2; AW554328
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC048184 sequence for NM_025830
CGGAAGTGGAGCGGAGTTAGGCGCCGCTGGTGTGGCCGCGGCAGTTCCATTGTGATGACATGGCATCTGC CAGCTCCAGCCGGGCAGGAGTGGCCCTGCCTTTTGAGAAGTCCCAGCTTACCCTGAAAGTGGTGTCAGCA AAGCCCAAGGTGCACAACCGCCAGCCCAGAATCAACTCCTACGTGGAGGTGGCAGTGGATGGACTCCCCA GCGAGACCAAGAAGACGGGGAAGCGCATCGGGAGCTCTGAACTGCTCTGGAATGAAATCATCGTTCTGAA TGTCACAGCCCAGAGTCATTTGGATCTGAAGGTCTGGAGCTGTCATACCTTGAGGAATGAACTACTGGGC ACTGCCTCTGTCAACCTCTCCAATGTCCTGAAGAACAATGGCGGCAAAATGGAGAACACACAACTGACCC TGAACCTGCAGACAGAGAACAAAGGCAGTGTTGTCTCGGGAGGAGAGCTGACAATTTTCCTGGATGGGCC AACTGTTGATCTGGGAAGTGTGCCTAATGGCAGTGCAGTGACAGACGGATCACAGCCACCTTCAAGAGAA TCCAGTGGGACTGCTATAGCTCCAGAGACCCGGCACCAGCCCCCCAGTACAAACTGCTTTGGTGGCAGAT CCCGGACGCACAGACACTCAGGTGGCTCAGCCAGGACAGCCACAGCAGCCAGTGAACAAAGCCCTGGCGC TAGGAACCGCCACCGCCAGCCTGTGAAGAACTCTAGCAGCAGTGGCTTAGCCAATGGCACAGTGAATGAG GAACCTACTCCAGCCAGTGAACCTGAAGAATCGTCGGTTGTTGGTGTAACATCCCTGCCTGCAGCAGCCT TGAGTGTGTCCTCAAATCCCAACACAACATCTCTCCCTGCACAGTCCACACCAGCAGAGGGAGAGGAGGC CAGCACTTCCGGGACACAGCAGCTCCCTGCTGCCGCCCAGGCCCCTGATGCTCTTCCTGCTGGATGGGAA CAGAGAGAGCTGCCCAATGGGCGTGTCTATTATGTTGATCACAACACCAAGACCACCACCTGGGAGCGGC CTCTTCCTCCAGGGTGGGAAAAGCGCACGGACCCCCGAGGGAGGTTTTACTACGTGGACCACAACACCCG GACAACCACCTGGCAGCGCCCAACTGCTGAGTACGTGCGCAACTATGAGCAGTGGCAGTCCCAGCGGAAC CAGCTGCAGGGGGCCATGCAGCACTTCAGCCAAAGATTCCTCTACCAGTCTTCGAGTGCTTCGACTGACC ATGATCCCTTGGGCCCCCTCCCACCTGGCTGGGAGAAGAGGCAGGACAATGGACGGGTGTATTATGTCAA CCACAACACTCGCACTACCCAGTGGGAGGACCCTCGGACCCAGGGGATGATACAGGAGCCAGCCCTGCCC CCAGGGTGGGAGATGAAATACACCAGCGAGGGCGTGCGGTACTTTGTGGACCACAATACCCGCACCACTA CCTTTAAGGATCCTCGCCCAGGGTTCGAGTCAGGGACAAAGCAAGGCTCACCTGGTGCCTATGACCGAAG TTTTCGGTGGAAGTATCACCAGTTCCGTTTCCTCTGCCACTCAAATGCTCTACCCAGCCATGTGAAGATC AGCGTTTCCAGGCAGACACTCTTTGAGGATTCTTTCCAACAGATTATGAACATGAAACCTTACGACCTGC GCCGCCGGCTCTACATCATCATGCGTGGTGAGGAGGGCCTGGACTACGGCGGCATCGCCAGAGAGTGGTT TTTCCTCCTGTCCCATGAGGTGCTCAACCCTATGTACTGTTTGTTTGAATATGCTGGGAAGAACAATTAC TGCCTGCAGATCAACCCGGCCTCTTCCATCAACCCTGACCACCTCACCTACTTCCGCTTTATCGGCAGAT TCATCGCCATGGCTCTGTACCATGGGAAGTTCATCGACACAGGCTTCACTCTCCCTTTCTACAAGCGGAT GCTCAACAAGAGACCGACTCTGAAGGACCTGGAGTCTATTGACCCTGAGTTTTACAACTCCATTGTCTGG ATCAAAGAGAACAACCTGGAAGAGTGTGGTCTGGAGCTGTTTTTCATCCAGGACATGGAGATTCTGGGCA AGGTGACAACCCATGAACTGAAGGAGGGCGGTGAGAACATCCGAGTTACCGAGGAGAACAAGGAGGAGTA TATCATGCTGCTGACTGACTGGCGATTCACCCGAGGCGTGGAAGAGCAGACCAAAGCTTTCCTGGATGGC TTCAATGAGGTTGCCCCTCTGGAGTGGTTGAGATATTTTGATGAGAAAGAGCTGGAGCTCATGCTCTGCG GCATGCAGGAGATAGACATGAGCGACTGGCAGAAGAACGCCATCTATCGGCACTACACCAAGAGCAGCAA GCAGATCCAGTGGTTCTGGCAGGTTGTCAAGGAGATGGACAATGAGAAGAGGATCCGGCTACTGCAGTTT GTCACGGGAACCTGCCGTCTGCCTGTTGGGGGATTTGCTGAGCTCATCGGGAGCAATGGCCCGCAGAAGT TCTGCATCGACAGAGTTGGCAAGGAAACCTGGCTGCCCAGGAGCCATACGTGCTTCAACCGTCTGGACCT GCCTCCCTATAAGAGCTACGAGCAGCTGAAAGAGAAGCTGCTGTACGCCATCGAGGAGACTGAGGGGTTC GGACAGGAGTAGCTGAGGCTGCTGTCTCACACCCTCTAGCACATGTAGTCCTGAGTCCTTCCTGCCGGAG ATGCCACCGGTTGCACAGCCCTCAAGAGGCCCCTTTGGATAGTGGCCCTGCACGAGACTGTGCCTTGATC ATTTCGGTGACTGAAGAGTCTGACCCCAGAGGCCTTGGGGACCCTTTCTTCTCTCTGATGATGGCAGTCT GGAATAAAGCACTGTCACCTTTGACTCTATTACCTGTTGCAAAGCCAGAGGGCTGCCCTTCTCTGCAAAA CTGCCCCTTCCACTAGGACTGAGTCTGGGTGTGTGTGAGAGTGTGAGGGAACATGCCCTGCCTTAGAGGG CTGCCACTGAAGCTGGGCCTCATATGTTCCATGAGGAGCTAGGCCTCGTGGAATGGCTGGCTGACTAAAC CCAGAAAGCCAAGGCTCTGCCAGTGGAAGAGACTTCACTTTTGTAGACCTTCCCTGAAGTCCATGGAACA GACCTAAGGGAACAACTGAGCATCAAAACCGGCTTTGCTTTACTTCCTGGCAGGCTGCAGCCAAGGGGAC TGAGCAGCAGGCACCCAATGCCACCAGCTCGTCTCTTCCTAGCAAGCAAAGGGTTAAAGAAGCCACCACC TGGGTGTCAGTGGGAAGGAGACAGCAGAGCTCAGGCTTTGCCCTTTGACAGAAACCTAAAGACACATCCA CATCCATGCATAGGGCTGTTGCTTGTTGTGTTGTAGGCATGCTCTTCTCACCTTCTGTCAAGGACCTGGT GCCCTGGGTGGTGGAGGGCTCAGCCCTCTTGATTCAGTAGCTTCCCAAGCAGCTGCTCCTCTCAGGAGCC TGCTTAACACTTGCGCCAGCTCAGAGGCCTGCCTCTATGGTCCAGTGCATCCCCGCTGTGCTCAGATGCT GACGAGACTCCTTTGCTAGACTCCCCTAGTTAGGGCCTCTGCAGCTTATGGTGGCTTAAAATGGAGAGAG CATCTTGCCTGGCCGAGTGTTTGTGCTATGTGAGCATGAGGCTTTATTTGACTGTGGAAGAAGACACTAA GAGCCAGCAGAAGGGCCTGAGCTGCCTCATTAGCCTGGCTTCATTTGGGGAGCTCCTGACAGTGTCCCGA GTAGGTGCCCAGCCCCTCTTGGGTACCAGAGTGGTTATAGCGACACACTCCTCATCCAGGCTTCCCAAGA TACACTAGCTCTGGGACACCCCAGGCATGCTCTCAAACTAGTCATGGGGCCTTCTGAGGACAGTTCCATC CTACCTTTGTCCTTCCCTGGTACAGCCTGTGACCCTGGCACACTGCACATGCTGATCTGAGTGGCTCTGC TCACTGTCGCACACCCCTTCCTCTCCCATGCAGTCACCTCAGGACTCCCGACATACATCACAAAGGCTGC TTCATGGCTGGGTGTGGGACCAGATGCCAGCAAGGATGGAAGCATCAGGCTCACAGGTGTGTGTGTGTGT GTGCACGCGTTCGCACATGCGTGAGTGTGTAGCAGAAGTGTGTACAGAGAGGCCCGTGCTAGCTAGCACC AAGTTGGTTGACAGATTGTTTTGTGATGCTTGCCCTGCCTCGGTATTGCCAGTTTCTTGTGCAATAAAGA ATCAGCAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_025830
Insert Size 2613 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC048184, AAH48184
RefSeq Size 4319 bp
RefSeq ORF 2613 bp
Locus ID 66894
UniProt ID Q9DBH0
Cytogenetics 8 D3
Summary E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.UniProtKB/Swiss-Prot Function
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"NM_025830" in other vectors (4)
SKU Description Size Price
MG211018 Wwp2 (tGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$997.00
MR211018 Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$589.00 $797.00
MR211018L3 Lenti ORF clone of Wwp2 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$1,097.00
MR211018L4 Lenti ORF clone of Wwp2 (mGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2) 10 ug
$1,097.00

file-alt Citations

Itch WW domains inhibit its E3 ubiquitin ligase activity by blocking E2-E3 transthiolation
Riling, Kamadurai, Kumar et al
J. Biol. Chem. (2015)
AI Summary: OriGene products contributed mouse Itch variant 1 (MC205232), mouse Ndfip1 (MG202546), mouse Nedd4 (MG222243), and mouse WWP2 (MC202685) cDNA constructs, which were used to express and study the regulatory mechanisms of Nedd4-family E3 ubiquitin ligases in this research.
Snippet: cDNA constructs used: mouse Itch variant 1 (MC205232, Origene), mouse Ndfip1 (MG202546, Origene), mouse Nedd4 (MG222243, Origene), mouse WWP2 (MC202685, Origene). These inserts were ligated into an N-terminal His- MBP tag pRSF-1B plasmid. ... Cite SaveSaving
Showing 1-1 of 1 papers.
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