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Dnm1 (BC034679) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR211008
Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $794.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnm1
Synonyms AI838169; Dnm; Ftfl; mKIAA4093
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR211008 representing BC034679.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGGCAACCGCGGCATGGAAGACCTCATCCCGCTGGTTAACCGGTTACAGGACGCCTTCTCCGCCATC
GGCCAGAACGCGGACCTCGACCTGCCGCAGATCGCCGTGGTAGGCGGCCAGAGCGCCGGCAAGAGCTCG
GTGCTGGAGAATTTCGTGGGCAGGGACTTCTTGCCCCGAGGATCTGGCATCGTCACCCGGCGTCCCCTG
GTCCTGCAGCTGGTTAATTCTACCACAGAATATGCCGAGTTCCTGCACTGCAAGGGGAAGAAATTCACC
GACTTCGAGGAGGTGCGCCTGGAGATCGAGGCTGAGACCGATCGAGTCACCGGCACCAACAAGGGCATT
TCGCCAGTGCCCATCAACCTGCGGGTCTACTCGCCCCATGTGCTGAACCTGACTCTAGTGGACCTGCCA
GGAATGACCAAGGTCCCAGTTGGGGACCAACCTCCTGATATCGAGTTCCAGATCCGGGACATGCTTATG
CAGTTCGTCACTAAGGAGAACTGCCTTATCCTGGCTGTGTCCCCTGCCAACTCGGATTTGGCCAACTCT
GATGCCCTCAAGATCGCTAAGGAGGTGGACCCCCAGGGTCAGCGCACCATTGGGGTCATCACCAAGTTG
GACCTGATGGACGAGGGCACAGATGCGCGGGACGTGCTAGAGAACAAGCTGCTCCCTTTGCGCAGAGGT
TACATCGGCGTGGTGAACCGGAGCCAGAAGGACATAGACGGCAAGAAGGACATCACAGCCGCCTTGGCT
GCAGAGCGCAAATTCTTCCTCTCTCACCCATCCTACCGCCACTTGGCTGACCGCATGGGCACACCCTAC
CTGCAGAAGGTCCTCAATCAGCAATTGACCAACCACATCCGGGACACACTGCCGGGACTTCGGAACAAG
CTGCAGAGCCAGCTGCTGTCCATTGAGAAGGAGGTGGACGAGTACAAGAACTTCCGACCGGATGACCCA
GCGCGCAAGACCAAGGCCCTGCTGCAGATGGTCCAGCAGTTTGCAGTGGACTTCGAGAAGCGCATCGAG
GGTTCTGGAGACCAGATTGACACTTACGAGCTGTCAGGTGGAGCCCGCATTAACCGGATCTTCCATGAA
CGCTTCCCCTTTGAGCTGGTTAAGATGGAGTTTGATGAGAAGGAACTGCGAAGGGAGATCAGCTATGCT
ATCAAAAATATCCATGGCATCAGAACGGGGCTCTTTACCCCAGACATGGCCTTTGAAACCATTGTGAAA
AAGCAGGTGAAGAAGATTCGAGAGCCGTGTCTCAAGTGTGTGGACATGGTTATCTCGGAGCTAATCAGC
ACGGTTAGACAGTGCACCAAGAAGCTGCAGCAATACCCGCGTCTGCGGGAGGAGATGGAGCGAATTGTG
ACCACCCACATCCGGGAACGTGAGGGCCGCACCAAGGAGCAGGTCATGCTTCTCATCGACATTGAGCTG
GCTTACATGAATACCAACCACGAAGACTTCATAGGCTTTGCCAATGCTCAGCAGAGAAGCAACCAGATG
AACAAGAAGAAGACTTCAGGGAACCAGGATGAGATTCTGGTCATTCGAAAGGGGTGGTTGACCATCAAC
AACATCGGCATCATGAAGGGAGGCTCCAAGGAGTACTGGTTTGTGCTGACTGCTGAGAATCTGTCCTGG
TACAAGGATGATGAGGAGAAAGAGAAGAAGTACATGCTGTCTGTGGACAATCTGAAGCTGCGTGATGTG
GAGAAGGGCTTCATGTCAAGCAAGCATATTTTTGCCCTCTTCAACACAGAGCAGAGGAATGTCTACAAG
GATTACCGGCAGCTGGAACTGGCCTGTGAGACACAGGAGGAGGTGGACAGTTGGAAGGCTTCCTTCCTG
AGGGCTGGCGTGTACCCTGAGCGTGTTGGGGACAAAGAGAAAGCCAGTGAGACCGAGGAGAACGGCTCT
GACAGCTTCATGCACTCGATGGACCCTCAGCTGGAGCGCCAGGTGGAGACCATCCGGAACCTGGTAGAC
TCGTACATGGCCATTGTCAACAAGACTGTGCGGGACCTCATGCCCAAGACCATCATGCACCTCATGATC
AACAACACCAAGGAGTTTATCTTCTCTGAGCTGCTGGCCAACCTGTACTCTTGCGGGGACCAGAACACA
CTGATGGAAGAATCGGCCGAGCAGGCTCAGCGGCGCGACGAGATGCTGCGCATGTACCACGCACTGAAG
GAGGCGCTCAGTATTATCGGCGACATCAACACGACCACCGTCAGCACGCCCATGCCCCCGCCCGTGGAC
GACTCCTGGCTGCAGGTGCAGAGCGTACCGGCCGGACGCAGATCGCCCACGTCCAGCCCCACGCCGCAG
CGCCGAGCCCCCGCCGTGCCCCCAGCCCGGCCCGGATCGCGGGGCCCTGCTCCTGGGCCTCCGCCTGCT
GGATCCGCCCTGGGGGGGGCGCCCCCCGTGCCCTCCAGGCCGGGGGCTTCCCCTGACCCCTTTGGCCCC
CCTCCCCAGGTGCCCTCGCGCCCCAACCGCGCCCCGCCTGGGGTCCCCAGCCTAGGAGCATGGCGACTA
AATTCACCACAGGGCAAGCATGAGAATCGCGCTGGCAAAGCCAGGCTC
ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Protein Sequence
>Peptide sequence encoded by MR211008
Blue=ORF Red=Cloning site Green=Tag(s)

MGNRGMEDLIPLVNRLQDAFSAIGQNADLDLPQIAVVGGQSAGKSSVLENFVGRDFLPRGSGIVTRRPL
VLQLVNSTTEYAEFLHCKGKKFTDFEEVRLEIEAETDRVTGTNKGISPVPINLRVYSPHVLNLTLVDLP
GMTKVPVGDQPPDIEFQIRDMLMQFVTKENCLILAVSPANSDLANSDALKIAKEVDPQGQRTIGVITKL
DLMDEGTDARDVLENKLLPLRRGYIGVVNRSQKDIDGKKDITAALAAERKFFLSHPSYRHLADRMGTPY
LQKVLNQQLTNHIRDTLPGLRNKLQSQLLSIEKEVDEYKNFRPDDPARKTKALLQMVQQFAVDFEKRIE
GSGDQIDTYELSGGARINRIFHERFPFELVKMEFDEKELRREISYAIKNIHGIRTGLFTPDMAFETIVK
KQVKKIREPCLKCVDMVISELISTVRQCTKKLQQYPRLREEMERIVTTHIREREGRTKEQVMLLIDIEL
AYMNTNHEDFIGFANAQQRSNQMNKKKTSGNQDEILVIRKGWLTINNIGIMKGGSKEYWFVLTAENLSW
YKDDEEKEKKYMLSVDNLKLRDVEKGFMSSKHIFALFNTEQRNVYKDYRQLELACETQEEVDSWKASFL
RAGVYPERVGDKEKASETEENGSDSFMHSMDPQLERQVETIRNLVDSYMAIVNKTVRDLMPKTIMHLMI
NNTKEFIFSELLANLYSCGDQNTLMEESAEQAQRRDEMLRMYHALKEALSIIGDINTTTVSTPMPPPVD
DSWLQVQSVPAGRRSPTSSPTPQRRAPAVPPARPGSRGPAPGPPPAGSALGGAPPVPSRPGASPDPFGP
PPQVPSRPNRAPPGVPSLGAWRLNSPQGKHENRAGKARL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Recombinant protein using MR211008 also available, TP511008
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC034679
ORF Size 2601 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq Size 3448 bp
RefSeq ORF 2603 bp
Locus ID 13429
Cytogenetics 2 22.09 cM
MW 97.8 kDa
Summary This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein is a GTPase which is required for membrane recycling, including vesicle endocytosis in neurons. It may also be involved in cellular fission via association with microtubules and actin filaments. Mutations in this gene have been shown to cause seizures. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2014
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"BC034679" in other vectors (4)
SKU Description Size Price
MC220643 Dnm1 (untagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865), (10ug) 10 ug
$874.00
MG211008 Dnm1 (tGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) 10 ug
$789.00 $994.00
MR211008L3 Lenti ORF clone of Dnm1 (Myc-DDK-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) 10 ug
$1,094.00
MR211008L4 Lenti ORF clone of Dnm1 (mGFP-tagged) - Mouse dynamin 1 (cDNA clone MGC:25354 IMAGE:4506865) 10 ug
$1,094.00

Plasmid Map for MR211008

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR211008
Circular map for MR211008

file-alt Citations

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