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Mcm4 (NM_008565) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210993
Mcm4 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 4 homolog (S. cerevisiae) (Mcm4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $790.00
5 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Mcm4 or NM_008565"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcm4
Synonyms 19G; AI325074; AU045576; Cdc21; mcdc21; Mcmd4; mKIAA4003
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210993 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGTCCCCGGCATCCACCCCGAGCCGCCGCAGCAGCCGACGCGGACGAGTCACCCCAACCCAGTCCC
TTCGAAGTGAGGAAAGCAGGTCGTCACCCAATCGGAGACGTAGAGGCGAAGATTCTTCCACCGGAGAGCT
ACTGCCAATGCCCACCTCACCAGGAGCCGACCTGCAGAGCCCACCTGCACAGAATGCCTTGTTTTCCAGC
CCTCCTCAGATGCATTCTTTAGCTATTCCTTTGGACTTTGATGTTAGTTCACCATTGACATATGGCACTC
CCAGCTCGCGAGTGGAAGGAACCCCAAGAAGTGGGGTGAGAGGCACACCTGTAAGGCAGAGGCCAGATCT
GGGCTCAGCACGAAAGGGTTTGCAGGTGGATCTGCAGTCTGATGGCGCAGCAGCAGAAGACATCGTACCA
AGTGAACAGTCTCTAGGCCAAAAGCTTGTGATTTGGGGAACAGATGTGAATGTGGCAACATGTAAAGAGA
ATTTTCAGAGATTCCTTCAGTGTTTTACTGATCCTCTGGCCAAAGAAGAAGAAAATGTTGGCATAGATAT
TACTCAACCTTTGTACATGCAACAACTTGGAGAGATTAATATTACAGGAGAGCCATTTTTAAATGTGAAC
TGCGAACACATAAAATCATTTAGCAAAAATCTGTATAGACAGCTCATCTCCTACCCACAGGAGGTTATAC
CAACCTTTGACATGGCTGTCAATGAGATCTTCTTTGACCGTTATCCTGACTCCATCTTAGAACATCAGAT
TCAAGTCAGACCTTTTAATGCGTTGAAGACAAAGAGTATGAGAAACTTGAATCCAGAAGACATTGATCAG
CTCATCACCATCAGTGGCATGGTCATCAGAACATCACAGCTGATTCCGGAGATGCAGGAGGCCTTTTTCC
AATGCCAAGTCTCTGCCCACACCACCCGGGTGGAGATAGATCGAGGCAGAATTGCTGAGCCCTGCAGTTG
TGTGCACTGCCACACTACCCACAGCATGGCACTGATCCACAACCGATCATTCTTCTCTGACAAGCAAATG
ATCAAACTTCAAGAGTCTCCTGAAGACATGCCTGCTGGGCAGACACCTCACACTATTGTCCTTTTTGCCC
ACAATGACCTTGTTGACAAGGTTCAACCAGGGGACAGAGTGAACGTCACAGGCATATATCGAGCAGTACC
AATTCGAGTTAATCCAAGAGTGAGCAACGTGAAGTCTGTCTATAAAACCCACATTGATGTCATTCATTAT
CGGAAAACGGATGCAAAACGTCTGCATGGCCTTGATGAAGAAGCAGAACAGAAACTTTTTTCAGAGAAAC
GTGTGAAATTGCTTAAGGAACTTTCCAGGAAGCCAGATATTTATGAGCGGCTTGCTTCAGCCTTGGCTCC
CAGCATTTATGAACATGAAGATATCAAAAAGGGAATCTTACTTCAGCTCTTTGGTGGAACAAGGAAGGAT
TTCAGTCACACTGGGAGGGGTAAATTCCGTGCTGAGATCAACATCCTTCTGTGTGGGGACCCTGGCACCA
GCAAGTCCCAGCTGCTACAGTATGTGTACAACCTGGTCCCCAGAGGCCAGTACACGTCTGGAAAAGGCTC
CAGTGCGGTCGGCCTCACCGCCTATGTGATGAAAGACCCTGAGACCAGGCAGCTTGTCCTCCAGACAGGT
GCCCTCGTCCTGAGTGACAATGGGATATGCTGCATCGATGAGTTTGACAAAATGAATGAAAGCACAAGGT
CTGTGCTGCATGAGGTCATGGAACAGCAGACTCTGTCCATTGCAAAGGCTGGGATCATCTGTCAGCTCAA
TGCGCGCACCTCTGTCCTGGCAGCAGCAAATCCTATTGAGTCTCAGTGGAATCCTAAAAAAACAACCATT
GAAAATATCCAACTACCGCACACATTGTTGTCAAGGTTTGATCTCATTTTCCTCATGCTAGACCCTCAGG
ATGAGGCATATGACCGGCGTCTAGCTCATCACCTGGTTTCATTGTACTACCAAAGTGAGGAGCAAGTGGA
GGAGGAGTTCCTGGACATGGCCGTGCTGAAAGACTACATTGCATATGCCCATAGTACCATCATGCCCCGA
CTGAGTGAGGAGGCCAGCCAGGCTCTCATTGAGGCTTATGTAAACATGAGGAAGATTGGGAGTAGCCGGG
GGATGGTTTCTGCTTACCCTCGACAGCTAGAGTCATTAATTCGCTTAGCAGAAGCCCATGCTAAAGTAAG
ATTTTCAAACAAAGTTGAAGCAATTGATGTGGAAGAGGCAAAACGCCTCCACCGGGAGGCTCTGAAGCAG
TCTGCAACTGACCCTCGTACTGGCATTGTGGATATTTCTATTCTTACTACAGGAATGAGTGCCACTTCTC
GTAAACGGAAAGAAGAATTAGCTGAAGCATTGAGAAAACTTATTTTATCTAAGGGTAAAACACCAGCCTT
AAAGTACCAACAGCTGTTTGAGGATATTCGGGGACAGTCTGACACAGCAATTACCAAGGACATGTTTGAA
GAAGCCCTGCGAGCTTTGGCTGATGATGATTTCCTAACAGTGACTGGGAAGACTGTCCGCCTGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210993 protein sequence
Red=Cloning site Green=Tags(s)

MSSPASTPSRRSSRRGRVTPTQSLRSEESRSSPNRRRRGEDSSTGELLPMPTSPGADLQSPPAQNALFSS
PPQMHSLAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGSARKGLQVDLQSDGAAAEDIVP
SEQSLGQKLVIWGTDVNVATCKENFQRFLQCFTDPLAKEEENVGIDITQPLYMQQLGEINITGEPFLNVN
CEHIKSFSKNLYRQLISYPQEVIPTFDMAVNEIFFDRYPDSILEHQIQVRPFNALKTKSMRNLNPEDIDQ
LITISGMVIRTSQLIPEMQEAFFQCQVSAHTTRVEIDRGRIAEPCSCVHCHTTHSMALIHNRSFFSDKQM
IKLQESPEDMPAGQTPHTIVLFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIHY
RKTDAKRLHGLDEEAEQKLFSEKRVKLLKELSRKPDIYERLASALAPSIYEHEDIKKGILLQLFGGTRKD
FSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTG
ALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTI
ENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVSLYYQSEEQVEEEFLDMAVLKDYIAYAHSTIMPR
LSEEASQALIEAYVNMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRFSNKVEAIDVEEAKRLHREALKQ
SATDPRTGIVDISILTTGMSATSRKRKEELAEALRKLILSKGKTPALKYQQLFEDIRGQSDTAITKDMFE
EALRALADDDFLTVTGKTVRLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008565
ORF Size 2586 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008565.2, NP_032591.2
RefSeq Size 3589 bp
RefSeq ORF 2589 bp
Locus ID 17217
UniProt ID P49717
Cytogenetics 16 10.09 cM
MW 96.7 kDa
Summary Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008565" in other vectors (4)
SKU Description Size Price
MC200646 Mcm4 (untagged) - Mouse minichromosome maintenance deficient 4 homolog (S. cerevisiae) (Mcm4), (10ug) 10 ug
$790.00
MG210993 Mcm4 (tGFP-tagged) - Mouse minichromosome maintenance deficient 4 homolog (S 10 ug
$990.00
MR210993L3 Lenti ORF clone of Mcm4 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 4 homolog (S. cerevisiae) (Mcm4) 10 ug
$1,090.00
MR210993L4 Lenti ORF clone of Mcm4 (mGFP-tagged) - Mouse minichromosome maintenance deficient 4 homolog (S. cerevisiae) (Mcm4) 10 ug
$1,090.00

Plasmid Map for MR210993

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210993
Circular map for MR210993

file-alt Citations

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