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1300001I01Rik (BC072573) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210966
1300001I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1300001I01 gene (cDNA clone MGC:99410 IMAGE:5705116)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP
$589.00 $784.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1300001I01Rik
Synonyms 1300001I01Rik; Kiaa0664; mKIAA0664
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210966 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCATCAAGACGGACGAGTTGCCGGCGGCCGCCCCGGCCGACAGCGCCCGGGAGCACGGCTCGCAGG
CCGGCGGCAAGGGGCGGCCGAACGCGGCTGATCTCCCCTCGGTCATGCTGTTGAATGGTGACTGCTCAGA
GAATTTGAAGAAGGAAGAAGGGACATCTGAGCCACCTAGGGAAAATGGGCTAGATGAGGGTGAGCCTGGA
GATGAGACCACTGGACAGGAAGTCATTGTCATTCAGGACACAGGCTTTTCTGTGAAGATTCTGGCCCCTG
GCATCGAGCCCTTTTCCCTGCAGGTGTCCCCCCAGGAGATGGTGCAGGAGATCCACCAGGTGCTCATGGA
CCGTGAGGATACTTGTCATCGTACCTGCTTCTCACTACACTTGGATGGCAATATGCTGGACCACTTTTCA
GAGCTACGCAGTGTGGAGGGGCTGCAGGAGGGCTCGGTGCTCCGAGTTGTGGAAGAGCCTTACACGGTGC
GTGAGGCCCGAATCCATGTGCGTCATGTCAGAGACCTCCTCAAGAGCTTGGACCCATCTGATGCCTTCAA
TGGAGTTGACTGTAACTCCTTGTCCTTCCTGAGTGTCTTCACTGATGGTGACTTAGGAGACAGTGGGAAG
CGGAAGAAAGGCTTGGAGATGGACCCCATCGACTGCACCCCACCTGAGTATATCCTGCCGGGGAGCCGGG
AGCGGCCTCTCTGTCCCCTGCAGCCCCAGAACCGTGACTGGAAGCCCCTGCAGTGTCTGAAAGTACTTAC
CATGAGTGGCTGGAACCCACCTCCTGGGAACCGCAAGATGCATGGGGACCTCATGTACCTGTTTGTGATC
ACTGCTGAGGACCGGCAAGTCAGCATCACTGCATCGACGAGGGGCTTCTACCTGAATCAGTCCACGGCCT
ACCACTTCAACCCCAAGCCTGCCAGCCCCCGCTTCCTCAGCCATTCCCTAGTAGAGCTGCTCAACCAGAT
CAGCCCAACCTTCAAAAAGAACTTTGCTGTGCTGCAGAAGAAAAGGGTCCAGCGCCACCCATTCGAGAGG
ATCGCCACCCCGTTCCAGGTATACAGCTGGACAGCCCCCCAGGCAGAGCATGCCATGGACTGTGTGCGTG
CGGAGGACGCCTACACCTCGAGGCTGGGCTACGAGGAGCACATTCCTGGACAGACCCGGGACTGGAATGA
AGAGCTGCAGACAACAAGGGAGCTGCCCCGCAAGAACCTGCCTGAGCGGTTGCTCCGAGAACGAGCCATA
TTCAAGGTGCACAGTGATTTCACAGCGGCAGCCACAAGGGGTGCCATGGCAGTTATTGATGGCAATGTGA
TGGCCATCAACCCCAGTGACGAGACCAAGATGCAGATGTTCATCTGGAACAACATCTTCTTCAGCCTGGG
CTTTGATGTCCGGGACCACTACAAAGACTTTGGTGGGGATGTGGCAGCCTACGTGGCACCCACCAATGAC
CTGAATGGTGTTCGCACATACAATGCCGTGGATGTAGAGGGACTGTACACACTGGGTACAGTGGTAGTGG
ATTACCGTGGCTACCGGGTCACAGCTCAGTCCATCATCCCTGGCATCCTGGAACGAGACCAGGAGCAGAG
TGTCATCTATGGCTCCATTGACTTTGGCAAGACGGTGGTGTCTCACCCACGCTATCTGGAGCTGTTGGAG
CGCACCAGCCGGCCCCTCAAGATCCTTCGTCACCGAGTACTCAATGACCGAGACGAGGAGGTGGAGCTCT
GCTCCTCGGTCGAGTGTAAGGGCATCATTGGCAATGATGGGCGCCACTACATCCTTGACCTACTGCGCAC
CTTCCCACCAGACCTCAACTTCCTTCCTGTGCCTGGTGAGGAGCTACCCGAGGAGTGCTCTCGAGCTGGC
TTCCCCCGAACCCATCGACACAAGCTGTGCTGTTTGCGCCAGGAGCTAGTGGATGCTTTTGTGGAGCACA
GGTATCTTCTCTTCATGAAGCTGGCTGCCTTACAGTTGATGCAACAGAAGGCCAGCAAGGTGGAGACCAC
CACCTCCCTGGAGAATGGTGGCCTTCCCTCCTCAGCAGAGACCAAATCTGAAGATTCAATAGGACCTGAA
GCAGGATGTGAGGAGGAGGGCAGCAGTGTGAGTGGCCTCGCCAAGGTGAAGGAGCTGGCAGAGACCATCG
CCTCAGATGATGGCACAGTAGACCCTCGAAGCCGAGAGGTGATCCGAAATGCCTGCAAGGCTGTTGGCTC
CATCAGCAGCACGGCCTTTGATATTCGCTTCAATCCTGACATCTTCTCCCCAGGGGTTCGATTTCCTGAG
TCCTGCCAGGATGAAGTTCGGGACCAGAAGCAGCTGTTGAAAGATGCTGCTGCTTTCCTGCTCTCCTGCC
AGATCCCCGGTTTGGTGAGGGAGGGGCTGAAAGGTTGGGTTGGGGCTGCTGAAAGGGGCTGTTGCCCCTT
GGTGCCTCCTGTTCCTTGGTTTCTTGATCACATAGAAAACCTTCTTGTAACTTCTGCATTGGCCTCTCCT
AGCCTGGCACACTGGCAGTGCCCAATAAGTCAAAGGAGGCGTCAGATA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210966 protein sequence
Red=Cloning site Green=Tags(s)

MVIKTDELPAAAPADSAREHGSQAGGKGRPNAADLPSVMLLNGDCSENLKKEEGTSEPPRENGLDEGEPG
DETTGQEVIVIQDTGFSVKILAPGIEPFSLQVSPQEMVQEIHQVLMDREDTCHRTCFSLHLDGNMLDHFS
ELRSVEGLQEGSVLRVVEEPYTVREARIHVRHVRDLLKSLDPSDAFNGVDCNSLSFLSVFTDGDLGDSGK
RKKGLEMDPIDCTPPEYILPGSRERPLCPLQPQNRDWKPLQCLKVLTMSGWNPPPGNRKMHGDLMYLFVI
TAEDRQVSITASTRGFYLNQSTAYHFNPKPASPRFLSHSLVELLNQISPTFKKNFAVLQKKRVQRHPFER
IATPFQVYSWTAPQAEHAMDCVRAEDAYTSRLGYEEHIPGQTRDWNEELQTTRELPRKNLPERLLRERAI
FKVHSDFTAAATRGAMAVIDGNVMAINPSDETKMQMFIWNNIFFSLGFDVRDHYKDFGGDVAAYVAPTND
LNGVRTYNAVDVEGLYTLGTVVVDYRGYRVTAQSIIPGILERDQEQSVIYGSIDFGKTVVSHPRYLELLE
RTSRPLKILRHRVLNDRDEEVELCSSVECKGIIGNDGRHYILDLLRTFPPDLNFLPVPGEELPEECSRAG
FPRTHRHKLCCLRQELVDAFVEHRYLLFMKLAALQLMQQKASKVETTTSLENGGLPSSAETKSEDSIGPE
AGCEEEGSSVSGLAKVKELAETIASDDGTVDPRSREVIRNACKAVGSISSTAFDIRFNPDIFSPGVRFPE
SCQDEVRDQKQLLKDAAAFLLSCQIPGLVREGLKGWVGAAERGCCPLVPPVPWFLDHIENLLVTSALASP
SLAHWQCPISQRRRQI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC072573
ORF Size 2568 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC072573, AAH72573
RefSeq Size 6121 bp
RefSeq ORF 2570 bp
Locus ID 74148
Cytogenetics 11
MW 95.6 kDa
Summary mRNA-binding protein involved in proper cytoplasmic distribution of mitochondria. Specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm and regulates transport or translation of these transcripts close to mitochondria, playing a role in mitochondrial biogenesis.UniProtKB/Swiss-Prot Function
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Resources
"BC072573" in other vectors (4)
SKU Description Size Price
MC220837 1300001I01Rik (untagged) - Mouse RIKEN cDNA 1300001I01 gene (cDNA clone MGC:99410 IMAGE:5705116), (10ug) 10 ug
$863.00
MG210966 1300001I01Rik (tGFP-tagged) - Mouse RIKEN cDNA 1300001I01 gene (cDNA clone MGC:99410 IMAGE:5705116) 10 ug
$984.00
MR210966L1 Lenti ORF clone of 1300001I01Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1300001I01 gene (cDNA clone MGC:99410 IMAGE:5705116) 10 ug
$1,084.00
MR210966L2 Lenti ORF clone of 1300001I01Rik (mGFP-tagged) - Mouse RIKEN cDNA 1300001I01 gene (cDNA clone MGC:99410 IMAGE:5705116) 10 ug
$1,084.00

Plasmid Map for MR210966

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210966
Circular map for MR210966

file-alt Citations

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