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Hspa4 (NM_008300) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210917
Hspa4 (Myc-DDK-tagged) - Mouse heat shock protein 4 (Hspa4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$589.00 $771.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hspa4
Synonyms 70kDa; AI317151; APG-2; Hsp70RY; Hsp110; mKIAA4025
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210917 representing NM_008300
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGTGGTGGGCATAGACCTGGGCTTTCAGAGCTGCTATGTCGCTGTGGCCCGCGCCGGCGGCATCG
AGACGATCGCTAACGAGTATAGCGACCGCTGCACGCCGGCATGTGTTTCTTTTGGTCCTAAGAATCGTTC
AATTGGAGCAGCAGCTAAGAGCCAGGTAATTTCTAATGCCAAGAACACAGTTCAAGGCTTTAAAAGATTC
CATGGGCGTGCATTCTCCGATCCATTTGTGGAAGCAGAAAAATCTAATCTTGCATATGATATTGTGCAGT
TGCCCACTGGATTAACGGGCATAAAGGTTACATATATGGAAGAAGAGAGGAATTTTACCACGGAACAAGT
GACTGCCATGCTTTTGTCGAAACTGAAGGAGACAGCAGAGAGTGTTCTTAAGAAGCCTGTGGTAGACTGT
GTTGTCTCGGTTCCTAGTTTCTACACTGATGCAGAACGGCGGTCAGTGATGGATGCCACACAGATTGCTG
GTCTCAATTGTTTGCGGTTAATGAATGAAACAACTGCAGTTGCTCTTGCTTATGGAATCTATAAACAAGA
CCTTCCTGCCTTAGAAGAAAAACCAAGAAATGTAGTTTTTGTAGATATGGGACATTCTGCCTATCAAGTT
TCTGTATGTGCATTTAATAAAGGAAAACTGAAAGTTTTGGCCACAGCTTTTGATACAACATTGGGAGGCA
GAAAATTTGATGAGGTGTTGGTAAATCACTTCTGTGAAGAATTTGGAAAGAAATACAAGCTAGACATAAA
ATCTAAAATTCGTGCTTTATTACGACTGTCTCAAGAGTGTGAGAAACTCAAGAAATTGATGAGTGCGAAT
GCTTCAGACCTCCCTTTGAGCATTGAGTGTTTTATGAATGATATTGATGTGTCCGGAACTATGAATAGAG
GCAAATTTTTGGAGATGTGTGATGATCTCCTAGCAAGAGTGGAGCCGCCACTTCGAAGCGTCTTGGAGCA
ATCCAAGTTAAAGAAAGAAGATATTTATGCAGTGGAGATAGTTGGTGGTGCCACACGAATCCCTGCAGTG
AAAGAGAAGATCAGCAAGTTTTTTGGTAAAGAACTCAGCACAACATTAAATGCAGATGAAGCTGTCACTC
GAGGCTGTGCTCTGCAGTGTGCAATTTTATCACCTGCTTTCAAAGTCAGAGAATTTTCTATCACTGATGT
GGTACCATATCCAATATCTTTGCGATGGAATTCTCCAGCTGAAGAGGGGTTAAGTGACTGTGAAGTCTTC
CCCAAAAACCATGCTGCTCCTTTCTCCAAAGTCCTCACCTTCTACAGAAAGGAACCTTTCACTCTTGAGG
CCTACTATAGTTCTCCTCAGGATTTGCCCTATCCAGATCCTGCTATAGCTCAGTTCTCAGTTCAGAAAGT
CACTCCACAGTCTGATGGCTCCAGCTCAAAAGTGAAAGTCAAGGTCCGGGTGAACGTGCACGGCATCTTC
AGTGTGTCCAGTGCGGCCTTGGTGGAGGTGCACAAGTCTGAAGAGAGTGAGGAGCCCATGGAGACGGATC
AGAATGCAAAGGAAGAGGAGAAGATGCAAGTGGACCAGGAAGAACCACATACTGAAGAGCAGCAGCAGCA
GCCACAGACACCAGCAGAAAACAAGGCGGAGTCTGAGGAGATGGAGACATCTCAAGCTGGATCAAAAGAT
AAAAAGACGGACCAACCACCTCAAGCAAAGAAGGCAAAAGTGAAGACCAGCACTGTGGACCTGCCCATTG
AGAGCCAGCTGCTCTGGCAGCTGGACCGGGAGATGCTCGGCCTGTACACAGAGAATGAGGGTAAGATGAT
CATGCAGGATAAACTGGAGAAGGAACGGAATGACGCCAAGAATGCTGTGGAAGAGTATGTGTATGAGATG
AGAGACAAGCTTAGTGGTGAATATGAGAAGTTTGTGAGTGAAGATGATCGTAATACTTTTACCTTAAAAT
TAGAGGATACTGAAAATTGGCTGTATGAAGATGGAGAAGATCAACCAAAGCAAGTTTATGTAGACAAATT
GGCTGAATTAAAAAGTTTAGGTCAACCCATTAAGACACGGTTCCAGGAATCTGAAGAAAGACCAAAATTA
TTTGAAGAACTAGGGAAGCAAATCCAACAGTATATGAAAGTGATCAGCTCTTTCAAAAACAAGGAGGACC
AGTATGAACACTTGGATGCTGCTGACGTGACAAAGGTAGAGAAAAGCACCAATGAGGCGATGGAGTGGAT
GAATAGCAAGCTTAACCTGCAGAACAAACAGAGCCTGACGGTGGATCCAGTTGTCAAGACAAAGGAGATT
GAAGCTAAAATTAAGGAGCTGACAAGTATTTGCAGTCCTATAATTTCAAAACCCAAACCCAAAGTGGAAC
CCCCAAAGGAGGAACCAAAACATGCTGAGCAGAATGGACCAGTGGATGGCCAAGGAGACAACCCAGGCTC
CCAGGCTGCTGAGCACGGTGCAGACACAGCTGTGCCTTCTGATGGCGACAAGAAGCTTCCTGAGATGGAC
ATTGAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210917 representing NM_008300
Red=Cloning site Green=Tags(s)

MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACVSFGPKNRSIGAAAKSQVISNAKNTVQGFKRF
HGRAFSDPFVEAEKSNLAYDIVQLPTGLTGIKVTYMEEERNFTTEQVTAMLLSKLKETAESVLKKPVVDC
VVSVPSFYTDAERRSVMDATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKPRNVVFVDMGHSAYQV
SVCAFNKGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLKKLMSAN
ASDLPLSIECFMNDIDVSGTMNRGKFLEMCDDLLARVEPPLRSVLEQSKLKKEDIYAVEIVGGATRIPAV
KEKISKFFGKELSTTLNADEAVTRGCALQCAILSPAFKVREFSITDVVPYPISLRWNSPAEEGLSDCEVF
PKNHAAPFSKVLTFYRKEPFTLEAYYSSPQDLPYPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIF
SVSSAALVEVHKSEESEEPMETDQNAKEEEKMQVDQEEPHTEEQQQQPQTPAENKAESEEMETSQAGSKD
KKTDQPPQAKKAKVKTSTVDLPIESQLLWQLDREMLGLYTENEGKMIMQDKLEKERNDAKNAVEEYVYEM
RDKLSGEYEKFVSEDDRNTFTLKLEDTENWLYEDGEDQPKQVYVDKLAELKSLGQPIKTRFQESEERPKL
FEELGKQIQQYMKVISSFKNKEDQYEHLDAADVTKVEKSTNEAMEWMNSKLNLQNKQSLTVDPVVKTKEI
EAKIKELTSICSPIISKPKPKVEPPKEEPKHAEQNGPVDGQGDNPGSQAAEHGADTAVPSDGDKKLPEMD
ID

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008300
ORF Size 2526 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008300.3, NP_032326.3
RefSeq Size 4657 bp
RefSeq ORF 2529 bp
Locus ID 15525
Cytogenetics 11 31.91 cM
MW 94.2 kDa
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Documents
Resources
"NM_008300" in other vectors (4)
SKU Description Size Price
MC221995 Hspa4 (untagged) - Mouse heat shock protein 4 (Hspa4), (10ug) 10 ug
$849.00
MG210917 Hspa4 (tGFP-tagged) - Mouse heat shock protein 4 (Hspa4) 10 ug
$971.00
MR210917L3 Lenti ORF clone of Hspa4 (Myc-DDK-tagged) - Mouse heat shock protein 4 (Hspa4) 10 ug
$1,071.00
MR210917L4 Lenti ORF clone of Hspa4 (mGFP-tagged) - Mouse heat shock protein 4 (Hspa4) 10 ug
$1,071.00

Plasmid Map for MR210917

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210917
Circular map for MR210917

file-alt Citations

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