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Atp6v0a1 (BC071182) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210870
Atp6v0a1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $762.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atp6v0a1
Synonyms ATP6a1, Atpv0a1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210870 representing BC071182
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAGCTTTTCCGGAGTGAGGAGATGACGCTGGCCCAGCTATTTCTACAGTCTGAAGCTGCTTATT
GCTGTGTCAGTGAATTAGGAGAGCTTGGGAAGGTCCAATTTCGGGATTTAAATCCAGATGTGAATGTTTT
CCAGAGGAAATTTGTGAATGAAGTTAGAAGATGTGAAGAAATGGATCGAAAACTCCGATTTGTTGAGAAA
GAGATAAGGAAAGCTAACATCCCAATTATGGACACCGGTGAAAACCCAGAGGTGCCCTTCCCTCGGGACA
TGATCGACTTAGAGGCCAATTTCGAGAAGATTGAAAATGAACTGAAGGAAATCAACACTAACCAGGAAGC
TCTGAAGAGAAACTTCCTGGAACTGACTGAATTAAAATTTATCCTGCGAAAAACCCAGCAGTTTTTCGAT
GAGATGGCGGATCCAGACCTGTTGGAAGAGTCCTCATCACTCTTGGAGCCAAACGAGATGGGAAGAGGCG
CACCCTTACGACTTGGCTTTGTGGCTGGTGTGATTAACCGGGAGCGGATCCCGACCTTTGAGCGCATGCT
TTGGCGAGTGTGCCGAGGGAATGTGTTCCTGAGACAAGCTGAAATCGAAAACCCGCTGGAGGATCCTGTG
ACTGGCGACTACGTGCACAAGTCTGTGTTCATCATTTTTTTCCAAGGCGATCAGCTGAAAAACAGGGTCA
AGAAAATCTGTGAAGGGTTCCGAGCCTCCCTCTATCCCTGCCCTGAGACTCCACAGGAAAGGAAAGAAAT
GGCTTCCGGAGTCAATACCAGGATCGACGACCTCCAAATGGTTCTGAATCAGACGGAGGACCACCGCCAG
AGGGTTCTGCAGGCAGCTGCTAAGAACATCCGCGTCTGGTTCATCAAGGTGCGGAAGATGAAGGCTATCT
ACCATACCCTGAACCTGTGCAATATAGACGTGACCCAGAAGTGCCTGATCGCAGAAGTCTGGTGCCCTGT
CACTGACCTGGACTCCATCCAGTTTGCACTACGAAGAGGCACGGAACACAGTGGGTCCACTGTCCCCTCC
ATTCTGAACAGGATGCAGACAAACCAGACACCGCCGACATATAATAAAACCAACAAGTTCACGCACGGCT
TCCAGAACATAGTAGATGCTTATGGGATCGGCACTTACCGAGAGATTAACCCAGCTCCGTACACTGTCAT
CACCTTCCCCTTTCTGTTTGCTGTGATGTTCGGAGATTTTGGGCACGGCATTTTGATGACGCTGTTTGCT
GTGTGGATGGTGTTGAGGGAGAGCCGGATCCTCTCCCAGAAGCACGAGAATGAGATGTTTAGCATGGTGT
TCAGCGGCCGGTACATTATTCTTCTGATGGGACTGTTCTCCATCTATACTGGACTCATCTACAATGACTG
CTTTTCCAAGTCTCTGAATATCTTTGGGTCATCGTGGAGTGTACGGCCAATGTTCACTCAAGGGAACTGG
ACGGAGGAGACGCTTCTGGGGAGTTCTGTTCTCCAGTTGAACCCCGCTATCCCTGGAGTTTTTGGTGGCC
CGTACCCGTTTGGCATTGATCCGATTTGGAACATTGCAACCAACAAGCTGACCTTCCTCAACTCCTTCAA
GATGAAGATGTCTGTTATCCTCGGCATCATCCACATGCTGTTTGGAGTCAGCCTGAGCCTTTTCAACCAT
ATCTATTTCAAGAAGCCCCTGAACATCTACTTTGGCTTTATTCCTGAGATCATCTTCATGTCCTCGTTGT
TTGGCTACCTGGTCATCCTTATCTTTTACAAGTGGACAGCCTACGATGCCCACTCGTCTAGGAATGCCCC
GAGCCTCCTGACCCACTTCATAAACATGTTCCTCTTCTCCTACCCAGAGTCTGGTAATGCAATGCTGTAC
TCTGGACAGAAAGGAATTCAGTGTTTCCTCATCGTGGTGGCGATGCTCTGCGTCCCTTGGATGCTTCTGT
TTAAGCCGCTGATCCTTCGCCATCAGTACCTGAGGAAGAAGCATTTGGGAACTCTCAACTTTGGTGGGAT
CAGGGTGGGCAACGGACCGACAGAGGAGGATGCTGAAATTATTCAGCATGACCAGCTTTCCACCCATTCA
GAGGACGCAGAAGAGTTTGACTTTGGCGACACCATGGTCCACCAGGCCATCCACACCATTGAGTACTGCT
TGGGTTGTATCTCCAACACCACGTCGTACCTGCGACTCTGGGCCCTCAGCCTGGCCCACGCACAGCTCTC
TGAAGTACTTTGGACCATGGTGATCCACATTGGCCTGCACGTCCGGAGTTTGGCCGGGGGTCTAGGGCTG
TTTTTCATCTTCGCCGCCTTTGCCACGCTGACCGTGGCTATCCTGCTGATCATGGAGGGCCTCTCGGCCT
TCCTCCACGCGCTGCGGTTACACTGGGTGGAGTTCCAGAACAAATTCTACACTGGGACTGGCTTCAAGTT
CCTCCCCTTCTCCTTTGAGCACATTCGGGAAGGGAAGTTTGATGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210870 representing BC071182
Red=Cloning site Green=Tags(s)

MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEK
EIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFD
EMADPDLLEESSSLLEPNEMGRGAPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPV
TGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQ
RVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPS
ILNRMQTNQTPPTYNKTNKFTHGFQNIVDAYGIGTYREINPAPYTVITFPFLFAVMFGDFGHGILMTLFA
VWMVLRESRILSQKHENEMFSMVFSGRYIILLMGLFSIYTGLIYNDCFSKSLNIFGSSWSVRPMFTQGNW
TEETLLGSSVLQLNPAIPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNH
IYFKKPLNIYFGFIPEIIFMSSLFGYLVILIFYKWTAYDAHSSRNAPSLLTHFINMFLFSYPESGNAMLY
SGQKGIQCFLIVVAMLCVPWMLLFKPLILRHQYLRKKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHS
EDAEEFDFGDTMVHQAIHTIEYCLGCISNTTSYLRLWALSLAHAQLSEVLWTMVIHIGLHVRSLAGGLGL
FFIFAAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYTGTGFKFLPFSFEHIREGKFDE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC071182
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC071182.1
RefSeq Size 3980 bp
RefSeq ORF 2498 bp
Locus ID 11975
Cytogenetics 11 64.04 cM
MW 145.9 kDa
Summary Required for assembly and activity of the vacuolar ATPase. Potential role in differential targeting and regulation of the enzyme for a specific organelle (By similarity).UniProtKB/Swiss-Prot Function
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"BC071182" in other vectors (4)
SKU Description Size Price
MC220706 Atp6v0a1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184), (10ug) 10 ug
$839.00
MG210870 Atp6v0a1 (tGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) 10 ug
$962.00
MR210870L3 Lenti ORF clone of Atp6v0a1 (Myc-DDK-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) 10 ug
$1,062.00
MR210870L4 Lenti ORF clone of Atp6v0a1 (mGFP-tagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit A1 (cDNA clone MGC:76459 IMAGE:30101184) 10 ug
$1,062.00

Plasmid Map for MR210870

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210870
Circular map for MR210870

file-alt Citations

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