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Grm4 (NM_001013385) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210868
Grm4 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 4 (Grm4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $763.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Grm4
Synonyms Gprc1d; mGluR4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210868 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGGAAGGGAGGCTGGGCCTGGTGGTGGGCCCGGCTGCCCCTCTGCCTACTCCTCAGCCTTTATG
GCTCCTGGGTGCCTTCATCCCTAGGAAAGCCCAAGGGTCACCCCCACATGAACTCTATCCGTATCGATGG
AGACATCACCCTGGGAGGCCTGTTTCCCGTCCACGGTCGCGGCTCCGAGGGCAAGGCCTGCGGCGAGTTG
AAGAAGGAGAAAGGTATCCACCGGCTGGAGGCCATGCTCTTTGCCCTGGACCGCATCAACAACGACCCGG
ACCTACTGCCCAACATCACGTTGGGCGCCCGCATTCTGGACACCTGCTCAAGGGATACCCACGCCCTGGA
GCAGTCCCTGACCTTTGTGCAGGCGCTCATCGAGAAGGACGGCACGGAGGTCCGCTGCGGCAGCGGGGGC
CCACCCATCATCACCAAGCCTGAACGAGTGGTGGGTGTCATCGGAGCTTCGGGGAGCTCCGTCTCGATCA
TGGTGGCCAACATCCTCCGCCTCTTCAAGATCCCCCAGATCAGCTACGCCTCCACGGCTCCCGACTTGAG
TGATAACAGCCGCTATGACTTCTTCTCCCGGGTCGTGCCCTCGGACACATACCAGGCCCAGGCCATGGTG
GACATCGTCCGGGCCCTCAAGTGGAACTATGTGTCCACGCTGGCCTCAGAGGGTAGCTACGGCGAGAGCG
GCGTGGAGGCCTTTATCCAGAAGTCCCGAGAGAACGGAGGCGTGTGCATTGCCCAGTCGGTGAAGATTCC
ACGGGAACCCAAGACCGGGGAGTTTGACAAGATCATCAAACGCCTTCTGGAAACGTCCAATGCCAGAGCC
ATCATCATCTTTGCCAACGAGGATGATATCAGGAGGGTGCTGGAGGCAGCGCGCAGGGCCAACCAGACCG
GCCACTTCTTTTGGATGGGTTCTGATAGCTGGGGCTCCAAGAGCGCCCCCGTGCTGCGCCTTGAGGAAGT
GGCTGAAGGTGCAGTCACCATTCTTCCCAAGAGGACGTCTGTGCGAGGGTTTGACCGATACTTCTCCAGC
CGCACGCTTGACAACAACAGGCGCAACATCTGGTTTGCTGAGTTCTGGGAGGACAACTTCCATTGCAAGT
TGAGCCGCCACGCGCTCAAGAAGGGAAGCCACATCAAGAAGTGCACCAACCGAGAGCGCATCGGGCAGGA
CTCGGCCTACGAACAGGAGGGGAAGGTGCAGTTTGTGATCGACGCCGTGTACGCCATGGGCCATGCTCTG
CACGCCATGCATCGTGACCTGTGTCCCGGCCGCGTAGGACTCTGCCCTCGAATGGACCCTGTGGATGGCA
CCCAGCTGCTTAAGTACATCAGAAACGTCAACTTCTCAGGCATCGCCGGGAACCCGGTGACCTTCAACGA
GAACGGAGACGCGCCAGGGCGTTATGACATCTACCAGTACCAACGTCGCAACGGCTCGGCTGAGTACAAG
GTCATCGGCTCATGGACAGACCACTTGCACCTCAGAATAGAGCGGATGCAGTGGCCAGGGAGTGGCCAGC
AGCTGCCACGCTCCATCTGCAGCCTGCCCTGCCAGCCAGGCGAGCGGAAGAAGACGGTGAAGGGCATGGC
TTGCTGCTGGCACTGCGAGCCCTGCACGGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACCTGC
CCCTATGACATGCGGCCCACGGAGAACCGCACGAGCTGCCAGCCCATACCCATTGTCAAGTTGGAGTGGG
ACTCACCCTGGGCTGTGCTGCCCCTCTTCCTGGCTGTGGTGGGCATTGCTGCCACGCTGTTCGTGGTGGT
CACTTTTGTGCGCTACAACGACACTCCGATCGTCAAGGCCTCGGGCCGGGAGCTGAGCTACGTCCTGCTG
GCGGGCATCTTTCTCTGCTATGCCACCACCTTCCTCATGATCGCAGAGCCTGACCTGGGGACCTGTTCAC
TCCGCCGCATCTTCCTGGGGCTTGGCATGAGCATCAGCTACGCGGCCCTGCTGACCAAGACCAACCGCAT
CTACCGCATCTTTGAGCAGGGCAAGCGGTCGGTCAGCGCCCCACGGTTCATCAGCCCCGCCTCGCAGCTG
GCCATCACCTTCGTCCTCATCTCGCTGCAGTTGCTTGGCATCTGCGTGTGGTTCGTGGTGGACCCCTCCC
ACTCGGTGGTGGACTTCCAGGACCAGCGGACACTTGACCCCCGCTTCGCCCGGGGTGTGCTCAAGTGTGA
CATCTCGGACCTGTCGCTCATCTGCCTCCTGGGCTACAGCATGCTGCTGATGGTCACGTGTACTGTGTAT
GCCATCAAGACTCGAGGCGTGCCTGAGACCTTCAATGAGGCCAAGCCCATCGGCTTCACCATGTACACCA
CCTGCATCGTCTGGCTGGCCTTCATCCCCATCTTTTTTGGCACCTCGCAGTCGGCTGACAAGGTAACCTC
TGAGGCCCTGCCCGTGGAATTCAGCCCGCCATTGCTGGCACATAAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210868 protein sequence
Red=Cloning site Green=Tags(s)

MSGKGGWAWWWARLPLCLLLSLYGSWVPSSLGKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKACGEL
KKEKGIHRLEAMLFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGG
PPIITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMV
DIVRALKWNYVSTLASEGSYGESGVEAFIQKSRENGGVCIAQSVKIPREPKTGEFDKIIKRLLETSNARA
IIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKSAPVLRLEEVAEGAVTILPKRTSVRGFDRYFSS
RTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHIKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHAL
HAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQRRNGSAEYK
VIGSWTDHLHLRIERMQWPGSGQQLPRSICSLPCQPGERKKTVKGMACCWHCEPCTGYQYQVDRYTCKTC
PYDMRPTENRTSCQPIPIVKLEWDSPWAVLPLFLAVVGIAATLFVVVTFVRYNDTPIVKASGRELSYVLL
AGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQL
AITFVLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVY
AIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSADKVTSEALPVEFSPPLLAHN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001013385
ORF Size 2496 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001013385.2
RefSeq Size 3231 bp
RefSeq ORF 2499 bp
Locus ID 268934
UniProt ID Q68EF4
Cytogenetics 17 A3.3
MW 92.8 kDa
Summary G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity (By similarity).UniProtKB/Swiss-Prot Function
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"NM_001013385" in other vectors (3)
SKU Description Size Price
MG210868 Grm4 (tGFP-tagged) - Mouse glutamate receptor, metabotropic 4 (Grm4) 10 ug
$963.00
MR210868L3 Lenti ORF clone of Grm4 (Myc-DDK-tagged) - Mouse glutamate receptor, metabotropic 4 (Grm4) 10 ug
$1,063.00
MR210868L4 Lenti ORF clone of Grm4 (mGFP-tagged) - Mouse glutamate receptor, metabotropic 4 (Grm4) 10 ug
$1,063.00

Plasmid Map for MR210868

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210868
Circular map for MR210868

file-alt Citations

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