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Usp43 (BC021474) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210839
Usp43 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $756.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Usp43
Synonyms 9130017K11Rik, B930051C24, RP23-464J2.1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGCAGAGGAAGGAGGTGTCCCTGCAGAGGAGGTGATCTTGGTTGAACTGTATCCCAATGGATTCC
AGCGGTCATTCTTTGATGAAGAGGACCTGAATACCATTGCAGAGGGTGACAATGTGTATGCTTTCCAAGT
CCCTCCCTCACCTGGTCTGGGGACGCTCTCAGCCCATCCGTCTGGTCTGTCAGTGTCACCGCGCTTGCCA
GTCCGTGACAGCCAGCGATTCTCTGGGCCTCTCCACAGCGAGAACAGGGTGGTCTTTCTCTTCTGTAACC
TGGTGGGCTCAGGGCAGCAGGCGAGCAGGTTTGGACCACCGTTCTTGATTCGGGAAGATAGAACAATCTC
CTGGGCCCAGCTTCAACAGTGTATTCTCAGCAAGGTTCGATGCCTCATGAGGAGTGAGGTCTCTGCACAG
GACCTGGGGACTCTGTTCTCCATCCGGGTTGTGGGGCTCTCCTTGGCCTGCAGCTACTTATCTCCAAAGG
ACAACCGGCCCCTTTGCCACTGGGCAGTTGACAGGGCTTTGCACCTCAGGAGGCCAGGAGGCCCTCCTCA
TGTCAAGCTGGCTGTGGAATGGGACAGCTCTGTCACAGAGCGCCTGTTTGGGAGCCTCCAGGAGGAACGG
GTCCAGGATGCAGACAGTGTGTGGCGGCAGCAGCAGGCACACCAGCAGCCCAGCTGTACCCTGGACGAAT
GTTTTCAGTCCTATACCAAGGAGGAACAGCTGGCCCAGGATGATGCATGGAAGTGCCCTCACTGCCAAGT
CCTCCAGCAGGGTGTGGTCAAGCTGAGTCTGTGGACTCTGCCCGACATCCTGATCATCCATCTGAAACGG
TTCTGTCAGGTGGGAGAGAGAAGGAACAAGCTCTCCACGCTGGTGAAGTTCCCACTGTCTGGACTCAACA
TGGCTCCCCATGTAGCACGGAGGAGCACCAACTCCAAGGCGGGGCCCGGCCCTTGGTCCTCCTGGAAGCA
GCCGATCTGCCTCCCCACCACCTACCCGCTGGACTTCCTCTACGACTTGTATGCTGTCTGCAACCACCAT
GGCAGTCTGCAAGGTGGGCATTACACAGCCTATTGCCGGAACTCCCTGGACGGCCAGTGGTACAGCTATG
ACGACAGCACAGTGGAGGCCCTGCGAGAGGATGAGGTCAACACCAGGGGGGCTTACATCCTATTCTACCA
GAAGCGGAACAGCATCCCTCCCTGGTCAGCCAGCAGCTCCATGAGAGGTTCCACCAGCTCCTCCTTGTCT
GATCATTGGCTCATGAGACTCGGGAGCCTCAACAACAGCACAAGGGGAAGCTTGCTGTCCTGGAGCTCGG
CCCCCTGCCCCTCCGTGGCCCGGGTACCCGACTCTCCTGTCTTCACCAATGGTGTCTGCCACCAGGACAA
GGGAGGAGTTGAGACCAGACCTTTGGTTCGGGGTGTTGGTGGCCGAAGTATCAGCATGAAGGCATCTCCT
GCTTCCCGATCCAGGCATGGGCCCTTCAAGACCATGCCCCTGAGGTGGTCATTTGGACACAGGGAGAAAC
GTCCCGGGGCATCCGTTGAACTGGTGGAATACCTAGAGTCCAGAAGAAGACCCCGTTCTACCAGCCAGTC
CATTGTGCCGCTCTTGACTCGTGCTGCTGGTGGGGAAGAGACTTCAGCGTCACCGAGGTCAGATGGCACC
CTTCCTGCTAAAAGTGAAGACAGCGGTCGAGCCATTGGGCAAGGAACAACTGGGGTGCCCCTTTCCTCAT
GCCACCTCAACCACCATCCAGCCCTGGGGTCTTTAGATGACAGTCTGCACACTGCCAGGACACGAACGGG
GAATGTAAGTCAGGATATCAGGCTCCCAAAAAAGTTTGACCTGCCCCTCACTGTGATGCCCTCAGTGGGG
GATGAAAAGCCAGCTCGCCCTGAGGGCCAGAAGATGACACCTTGGAAGGGAAGTAGCCAGGTGGGAAGCC
AGAGTAGCCCACCTTCCCCCTCCACTGGCTTGCTTAGAAACTTTAAGGACAATGGCCCAGGTACCTTACC
CAAGATGAAGTCTAAGGCTGCCATGGAGGAAAGGGCCCCTGGCAAAGACAGGGGACAGGGGACATTCACT
CTTTTGAAGTCTGTGTTTTGGAAGAAAGAACACAAGAGGACTGTCAGGACTGAGAGCTCACCACCAGCAC
CCCCAATCTCCCTAGGGAGTGACCGGCTGAGCCCTGCCATGAATGAGCAGGCCCGAGGTTCATCCCCTGC
CCTCAGGATTCGAGAGAGCCCAGCCAAGGGCCTGGGCAACCACATGGAGAGGGACATCAGATCTGCGCCC
AGCTCTCTCCACCTCCCCCGAAAAGCCAGCAGGCCCCCTCGAGCCAGTACAGCTGGCACCTCACAAAGGA
CCATTCCTGGGGAACAGATTTCTTATGGCACCCTGCAGAGAGTGAAATACCACACCCTTTCCTTAGGTCG
AAAGAAATCCTTACCTGAGTCCAGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210839 protein sequence
Red=Cloning site Green=Tags(s)

MVAEEGGVPAEEVILVELYPNGFQRSFFDEEDLNTIAEGDNVYAFQVPPSPGLGTLSAHPSGLSVSPRLP
VRDSQRFSGPLHSENRVVFLFCNLVGSGQQASRFGPPFLIREDRTISWAQLQQCILSKVRCLMRSEVSAQ
DLGTLFSIRVVGLSLACSYLSPKDNRPLCHWAVDRALHLRRPGGPPHVKLAVEWDSSVTERLFGSLQEER
VQDADSVWRQQQAHQQPSCTLDECFQSYTKEEQLAQDDAWKCPHCQVLQQGVVKLSLWTLPDILIIHLKR
FCQVGERRNKLSTLVKFPLSGLNMAPHVARRSTNSKAGPGPWSSWKQPICLPTTYPLDFLYDLYAVCNHH
GSLQGGHYTAYCRNSLDGQWYSYDDSTVEALREDEVNTRGAYILFYQKRNSIPPWSASSSMRGSTSSSLS
DHWLMRLGSLNNSTRGSLLSWSSAPCPSVARVPDSPVFTNGVCHQDKGGVETRPLVRGVGGRSISMKASP
ASRSRHGPFKTMPLRWSFGHREKRPGASVELVEYLESRRRPRSTSQSIVPLLTRAAGGEETSASPRSDGT
LPAKSEDSGRAIGQGTTGVPLSSCHLNHHPALGSLDDSLHTARTRTGNVSQDIRLPKKFDLPLTVMPSVG
DEKPARPEGQKMTPWKGSSQVGSQSSPPSPSTGLLRNFKDNGPGTLPKMKSKAAMEERAPGKDRGQGTFT
LLKSVFWKKEHKRTVRTESSPPAPPISLGSDRLSPAMNEQARGSSPALRIRESPAKGLGNHMERDIRSAP
SSLHLPRKASRPPRASTAGTSQRTIPGEQISYGTLQRVKYHTLSLGRKKSLPESSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC021474
ORF Size 2478 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021474, AAH21474
RefSeq Size 2948 bp
RefSeq ORF 2480 bp
Locus ID 216835
Cytogenetics 11 B3
MW 90.9 kDa
Summary May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins (By similarity).UniProtKB/Swiss-Prot Function
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"BC021474" in other vectors (4)
SKU Description Size Price
MC220699 Usp43 (untagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429), (10ug) 10 ug
$833.00
MG210839 Usp43 (tGFP-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) 10 ug
$956.00
MR210839L3 Lenti ORF clone of Usp43 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) 10 ug
$1,056.00
MR210839L4 Lenti ORF clone of Usp43 (mGFP-tagged) - Mouse ubiquitin specific peptidase 43 (cDNA clone MGC:38313 IMAGE:5343429) 10 ug
$1,056.00

Plasmid Map for MR210839

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210839
Circular map for MR210839

file-alt Citations

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