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Pign (BC021148) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210838
Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $756.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pign or BC021148"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pign
Synonyms Gm20308; PIG-N
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210838 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTGTTTTTTGCTTTGGGATTGCTTATCCATTTTGTGTTCTTCGCTTCCATCTTTGACATTTATT
TTACATCTCCTTTGGTTCATGGAATGACTCCCCAGTTTACACCTTTGCCTCCTCCAGCAAAAAGATTAGT
GCTATTTGTTGCTGATGGCCTGAGAGCAGATACTCTTTATGAATTAGATGAAGATGGAAACTCCAGAGCA
CCTTTCATTAGGAATGTTATAATTCATGAAGGCAGCTGGGGAGTATCTCACACACGTGTGCCAACAGAAT
CTCGGCCTGGCCATGTAGCCTTGATAGCTGGATTTTATGAGGATGTCAGTGCAGTTGCCAAAGGATGGAA
AGAAAATCCTGTAGAATTTGACTCTCTTTTTAATGAAAGCAAGTACACGTGGAGCTGGGGGAGTCCAGAT
ATCTTGCCCATGTTTGCCAAAGGTGCTAGTGGTGACCATGTCTATACATATAGTTACGATGCTCAAAGAG
AAGATTTTGGTGCCCACGATGCCACAAAACTGGATACGTGGGTTTTTGATAAAGTTAAGGACTTCTTTGA
TGCTGCAAGAAACAACCAATCTTTGTTCACAAAAGTAAATGAAGAAAAAGTAGTTTTTTTCTTACATTTA
TTGGGAATAGATACAAATGGACATGCTCATCGACCATCGTCAAGGGAATATAAGGACAATATTAAAAAAG
TTGATGATGGAGTGAAGGAAATCGTATCAATTTTCAAGCATTTTTATGGAGATGATGGGAAAACAGCATT
TATCTTTACCTCTGACCATGGAATGACAGACTGGGGTTCCCATGGGGCTGGTCATCCGTCAGAGACTTTA
ACTCCGTTCGTTACTTGGGGAGCTGGAATCAAATTTCCTCAAAATGTATCAGCTCAGCAATATGATGATG
AGTTTTTAAAAGAATGGAGATTGGAAAACTGGAAAAGGAGAGATGTCAATCAGGCTGATATTGCACCTCT
GATGGCATCCCTTATTGGAGTCCCCTTTCCTCTTAATTCGGTGGGAATACTTCCTGTGGGTTATCTTAAC
AACACTGGTCTCTTCAAAGCAGAGAGCATGTTTACCAATGCAGTACAGATTCTTGAACAGTTCAAGGTGA
AAATGACTCAGAAAAAAGAAGCTACTTTACCGTTTTTGTTTACACCATTTAAGTTGCTTTCTGATTCTCA
ACAACTGGACATTTTAAGAAAAGCAAGATCTTACATAAAACAAGAAAAATTTGATGAAGTAGTGTCCCTC
TGTGAGGAGCTGATTGACCTCGCCCTGAGAGGATTGTCCTATTATCATACTTATGACAGACTGTTTCTGG
GCATCAATGTTGCAGTTGGTTTTGTGGGATGGATGTCTTATACTTCTTTGTTAATCATCAAGTCGCATTC
TAATATCCCAAAAGGCACTAGGAAGGAAGGTAAGAAGCCACACTGTCTCCTGCTTTATAGCTTCATAGCT
ACTGGTGTCTTAGTAGCATGCTTCCTGATGATCCAAGCCTGTCCCTGGACTTACTATGTGTACTGCTTGC
TGCCAGTGCCTATATGGTATGCAGTTCTAAGAGAACATGAAGTTATTCAAGACCTGGTTGAATCACTATT
GACTTTTCCTCGGAGCCATTTTGTTGCCTACTTGCTTGTCTTTACCTTGGGTATTGAAGTACTGGTGCTC
AGTTTCTTCTACCGCTACATGTTGACAGCGGGACTGATTGTGTTTGCAGGCTGGCCGTTTCTCACTCAGC
TGTGGACCCGAGCAAAGATCACCTTCCTGAGTTGGGCTTTCTTCTCTTTGCTTCTGGCAGTGTTCCCACT
GATGCCTGTTGTGGGTAGAAAGCCAAACCTTTCTCTAGTAATGGGTGCTGGATTCCTGGTTCTTCTGCTG
TCGCTGGCTGTTGTAACAACTCTCGGAAAAAGAAACATTAAATTGGTAAAGGGCGAGCTGTTGGTACTTC
TGTTACAGATGCTGAGTACAGTGCTCTCTATGTATGTGGTGTATAGCACCCACCACAGTCTGTTAAAGAA
AGAAGGCCTGCCTCTTATGAATCAGATTGTGAGCTGGGCAACATTAGCCTCTTCTCTGGTGGCACCACTC
CTGAGCTCCACAGCTCTCTCTCAGCGACTGGCCAGCATACTCCTCTCCTTGATGTCCACCTACCTGCTCC
TAAGCACTGGGTATGAAGCTCTCTTTCCTTTAGTGCTGTCCTGTTTGATGTTTGTCTGGATCCAAGTGGA
ACAAGAGACCCTTCAACAGCCTGGTGTTTCTTGTAAACAGAAGCTCACCAGCATCCAGTTCACCTGTGAC
ACTGATATAGCTCAGTTCCGACAGCTCTGTCCGGATGACATCCGTAGGGCCTTTTTCCTCATATTGTTGA
TCTTTCCTGTATTCTTAAGACTATTCGACTACAAAAGAATAAGGATTCTGTTAGCCCAAGAAAATTATCA
TAGGTCATTAAAGGCTGCCTGGGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210838 protein sequence
Red=Cloning site Green=Tags(s)

MLLFFALGLLIHFVFFASIFDIYFTSPLVHGMTPQFTPLPPPAKRLVLFVADGLRADTLYELDEDGNSRA
PFIRNVIIHEGSWGVSHTRVPTESRPGHVALIAGFYEDVSAVAKGWKENPVEFDSLFNESKYTWSWGSPD
ILPMFAKGASGDHVYTYSYDAQREDFGAHDATKLDTWVFDKVKDFFDAARNNQSLFTKVNEEKVVFFLHL
LGIDTNGHAHRPSSREYKDNIKKVDDGVKEIVSIFKHFYGDDGKTAFIFTSDHGMTDWGSHGAGHPSETL
TPFVTWGAGIKFPQNVSAQQYDDEFLKEWRLENWKRRDVNQADIAPLMASLIGVPFPLNSVGILPVGYLN
NTGLFKAESMFTNAVQILEQFKVKMTQKKEATLPFLFTPFKLLSDSQQLDILRKARSYIKQEKFDEVVSL
CEELIDLALRGLSYYHTYDRLFLGINVAVGFVGWMSYTSLLIIKSHSNIPKGTRKEGKKPHCLLLYSFIA
TGVLVACFLMIQACPWTYYVYCLLPVPIWYAVLREHEVIQDLVESLLTFPRSHFVAYLLVFTLGIEVLVL
SFFYRYMLTAGLIVFAGWPFLTQLWTRAKITFLSWAFFSLLLAVFPLMPVVGRKPNLSLVMGAGFLVLLL
SLAVVTTLGKRNIKLVKGELLVLLLQMLSTVLSMYVVYSTHHSLLKKEGLPLMNQIVSWATLASSLVAPL
LSSTALSQRLASILLSLMSTYLLLSTGYEALFPLVLSCLMFVWIQVEQETLQQPGVSCKQKLTSIQFTCD
TDIAQFRQLCPDDIRRAFFLILLIFPVFLRLFDYKRIRILLAQENYHRSLKAAWEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC021148
ORF Size 2478 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021148, AAH21148
RefSeq Size 4038 bp
RefSeq ORF 2480 bp
Locus ID 27392
Cytogenetics 1 E2.1
MW 93.8 kDa
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"BC021148" in other vectors (4)
SKU Description Size Price
MC200937 Pign (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793), (10ug) 10 ug
$757.00
MG210838 Pign (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) 10 ug
$956.00
MR210838L3 Lenti ORF clone of Pign (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) 10 ug
$1,056.00
MR210838L4 Lenti ORF clone of Pign (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class N (cDNA clone MGC:13757 IMAGE:4024793) 10 ug
$1,056.00

Plasmid Map for MR210838

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210838
Circular map for MR210838

file-alt Citations

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