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Zfp28 (NM_175247) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210832
Zfp28 (Myc-DDK-tagged) - Mouse zinc finger protein 28 (Zfp28)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
  $831.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Zfp28
Synonyms 2810438M17Rik; mkr-5; Zfp-28
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210832 representing NM_175247
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGGAATGGCGAGTGTAGTGTCATGCGAACCCTGGGCCCTCCTGGGGCGTGGCGCCCTCTGCACAA
AGGCCAGGCCTGGTGGAGGTCCGGCGGCGGGGACTGTAGTTGCTCCTGGGAGCCCCGACCGGGGAAGGCC
GCGCTCCAGGAACAGCCTGGCCTCCCAGGATCAGCAAGGAGCAGTGACCTCGGGGACCGCGCACAAAGCT
CTGTTTTCCAGAGATACCAACTTCCTCCAGGAGATAAACCGCAAACAGGAAGCTGCCCCTACAGGGACTA
GACATAAAGCCAAGTCGCAGGGCTTGGTGACCTTTGGGGATGTGGCTGTAGTTTTCTCCCAGGAGGAGTG
GGAATGGCTCAACTCTGAGCAGAGGAGTCTGTACTGGAAGGTGATGCTGGACAACTACAGGAACCTAGCA
TCCCTGGGACTTTGTGCTTCTCAGCCAGATATGATCACCTCGTTGGAACAAGGAAGGGATCCCTGGATGA
TGAAAAGGAAGATGAGAAAAGGCCAGCACCTGGACTTGAAGGCCATGCAGGAGACCAAGGAGTTCCCTCC
AAAGGACTTAAGTGAGGAGACATTATTCCTGGCAGTGCTAAGAAAACAGCTCCTGCCCCATAGACCTAAG
TGCTCCATGGTACGGGCAGCCTGGGAGGGTGGTGCAGTGTTCACGACACACAGGGGCTTGAAGACCAACT
CAGGTCTGGCTAGAGACAGTCCTGCACAGCTTGTGTCAGCTCAGAGGAGTTTCTGTAAGAGTGTGACTTG
GGAGAACTGTGGTGACCGGGGCTCCGTAGGCCAGCAGTCTGTACAGGAAGCCCAGGATCTGCTTCCAAGG
CAGGACTCACATGCTGAACGGGTAACAGGCAGAACTTGGAGCACTAAACTTGAGTGCTCCACTTTCAGAG
ATCAGGATTCTGAGTGTACGTTTGAAAGGAATGAGCAAGAGACAGTCACTCCGAACAGAGCCTTCTCAGA
GGGGAGAGATGGTATGTGTATCGAATCTGGAAGATGGTTTCATTTGAACAGTTCAGACGAGAGAAGTCAT
AACTGTGACTCAGGTAAAAGTTTCTCCTCAAATCCAGTAGTTGTAAAGGAAACCGGAATCTGTTCAGGAA
AAAAACTTTTCCAATGTAATGAATGTAAGAAAACTTTCACCCAGAGCTCCTCCCTTACCGTTCACCAGAG
AATTCATACTGGAGAGAAGCCCTATAAATGCAATCAGTGTGGGAAGGCCTTCAGTGACGGGTCCTCCTTC
GCACGGCACCAGAGGTGCCATACAGGCAAGAAGCCGTACGAGTGCCCGGAGTGTGGCAAAGCTTTCATAC
AGAACACCTCGCTCGTTCGTCACTGGAGGTACTATCACACTGGGGAGAAACCCTTCGATTGCATCGACTG
TGGGAAAGCCTTCAGTGACCACATAGGGCTTAATCAACACAGGAGGATTCACACTGGAGAGAAACCATAC
ACGTGTGAAGTGTGTCACAAATCCTTTAGGTACGGCTCATCCCTCACTGTGCACCAAAGGATTCATACTG
GAGAGAAACCGTACGAGTGTGAGATTTGCAGAAAAGCCTTCAGCCACCATGCATCCCTCACTCAGCATCA
ACGAGTGCATTCTGGAGAAAAGCCTTTTAAATGTAAAGAGTGTGGGAAAGCTTTTAGGCAGAATATACAC
CTTGCTAGTCACTGGAGGATCCATACCGGGGAGAAGCCCTTTGAGTGTGGGGAATGTGGGAAATCTTTCA
GCATCAGCTCACAGCTTGCCACGCATCAGAGAATTCATACAGGAGAGAAGCCTTACGAATGTAAGGTTTG
TAGGAAAGCATTTACACAGAAGGCTCACCTTGCACAACATCAGAAGACTCATACGGGGGAGAAGCCATAC
GAGTGCAAGGAATGTGGCAAGGCCTTCAGCCAGACCACTCACCTCATTCAACACCAGAGGGTTCATACTG
GAGAGAAGCCCTATAAATGCCTGGAATGTGGGAAGGCCTTTGGTGATAACTCATCCTGTACTCAACATCG
GAGACTTCACACCGGCCAGAGGCCTTACGAGTGTGTGGAGTGTGGGAAGACATTCAAGACCAAGTCGTCC
CTTATCTGTCATCGTAGATGTCACACGGGAGAGAAACCTTATGAGTGCAGTGCATGTGGTAAAGCCTTTA
GCCACCGGCAGTCCCTTAGTGTACATCAGAGAATTCATTCGGGGAAGAAACCGTATGAATGTAAGGAATG
TCGGAAAACCTTCATCCAGATTGGACACCTTAACCAACATAAGAGAGTCCATACCGGGGAGAGAACGTAC
AACTACAAGAAGGGCAGGAGGGCCTTCAGGCAGACTGCACACTTTGCTCACCATCAGCAGATTCATTCCG
GAAAGTCACCTGCTCACCACTCTTTGCCTTCCACATCGAATCCTGTGGATCTCTTCTCCAAATTCGTCTG
GAATCCATCCTCACTGCCATCATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210832 representing NM_175247
Red=Cloning site Green=Tags(s)

MQGMASVVSCEPWALLGRGALCTKARPGGGPAAGTVVAPGSPDRGRPRSRNSLASQDQQGAVTSGTAHKA
LFSRDTNFLQEINRKQEAAPTGTRHKAKSQGLVTFGDVAVVFSQEEWEWLNSEQRSLYWKVMLDNYRNLA
SLGLCASQPDMITSLEQGRDPWMMKRKMRKGQHLDLKAMQETKEFPPKDLSEETLFLAVLRKQLLPHRPK
CSMVRAAWEGGAVFTTHRGLKTNSGLARDSPAQLVSAQRSFCKSVTWENCGDRGSVGQQSVQEAQDLLPR
QDSHAERVTGRTWSTKLECSTFRDQDSECTFERNEQETVTPNRAFSEGRDGMCIESGRWFHLNSSDERSH
NCDSGKSFSSNPVVVKETGICSGKKLFQCNECKKTFTQSSSLTVHQRIHTGEKPYKCNQCGKAFSDGSSF
ARHQRCHTGKKPYECPECGKAFIQNTSLVRHWRYYHTGEKPFDCIDCGKAFSDHIGLNQHRRIHTGEKPY
TCEVCHKSFRYGSSLTVHQRIHTGEKPYECEICRKAFSHHASLTQHQRVHSGEKPFKCKECGKAFRQNIH
LASHWRIHTGEKPFECGECGKSFSISSQLATHQRIHTGEKPYECKVCRKAFTQKAHLAQHQKTHTGEKPY
ECKECGKAFSQTTHLIQHQRVHTGEKPYKCLECGKAFGDNSSCTQHRRLHTGQRPYECVECGKTFKTKSS
LICHRRCHTGEKPYECSACGKAFSHRQSLSVHQRIHSGKKPYECKECRKTFIQIGHLNQHKRVHTGERTY
NYKKGRRAFRQTAHFAHHQQIHSGKSPAHHSLPSTSNPVDLFSKFVWNPSSLPSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_175247
ORF Size 2475 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175247.3, NP_780456.2
RefSeq Size 4161 bp
RefSeq ORF 2478 bp
Locus ID 22690
UniProt ID P10078
Cytogenetics 7 A1
MW 93.3 kDa
Summary May be involved in transcriptional regulation. May have a role in embryonic development.UniProtKB/Swiss-Prot Function
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Documents
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"NM_175247" in other vectors (2)
SKU Description Size Price
MC202406 Zfp28 (untagged) - Mouse zinc finger protein 28 (Zfp28), (10ug) 10 ug
$756.00
MG210832 Zfp28 (tGFP-tagged) - Mouse zinc finger protein 28 (Zfp28) 10 ug
$1,031.00

Plasmid Map for MR210832

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210832
Circular map for MR210832

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