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Osbpl3 (BC086485) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210802
Osbpl3 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $750.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Osbpl3
Synonyms ORP3, OSBP3, A530055M08
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210802 representing BC086485
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGACGAGAAGAATCTCGGTGTGTCTCAAAAGTTGGTTTCACCTTCAAGAAGCACAAGTAGTTGCT
CCTCCAAGCAAGGAAGCCGGCAGGACAGCTGGGAAGTCGTGGAAGGATTGAGAGGGGAAATGACTTACAC
CCAGGAGCCGCCAGTGCAGAAAGGATTTTTGCTGAAAAAGAGGAAGTGGCCCTTAAAAGGCTGGCACAAG
AGATTCTTCTGCCTGGAAAAGGGAATCTTGAAATATGCCAAGAGCCAAGCTGATATTGAGCGGGAGAAGT
TGCATGGCTGCATTGACGTGGGGCTCTCTGTGATGTCTGTGAAGAAGTCGTCTAAGTGTATCGACCTAGA
CACGGAGGAGCACATCTACCACCTGAAGGTGAAGTCAGAAGAACTCTTTGACGAGTGGGTGTCCAAGCTC
CGTCACCACAGAATGTACCGGCAGAATGAGATCGCCATGTTTCCCCGGGATGTCAATCACTTTTTCTCAG
GGTCCTCTGTCACAGATTCTGCCCCAGGAGTGTTCGAGTCAGTTTCCAGTAGGAAGCGTAGCAGTCTATC
AAAGCAGAATTCATTTCCACCCGGAAGCAATTTGTCATTTTCTTGTGGTGGCGACACTCGAGTTCCTTTC
TGGCTACAGTCTTCAGAGGACATGGAAAAATGTTCAAAAGACCTGGCACACTGCCATGCCTACCTGCTGG
AAATGAGCCAGCTCTTAGAAAGCATGGACGTCCTGCATCGGACATACTCGGCGCCAGCCATCAACGCCAT
CCAGGTCCCTAAGCCTTTTTCTGGCCCTGTGAGACTGCACTCCTCCAATCCTAACTTGTCGACGCTGGAC
TTTGGAGAAGAGAAGTCTTACTCGGATGGTTCTGAAGCCTCGTCGGAGTTCTCCAAGATGCAGGAGGATC
TGTGCCATGTCGCCCACAAAGTTTACTTCGCTTTAAGGTCGGCTTTCAATAGCATATCGGTGGAGAGAGA
GAAACTGAAGCAGCTGATGGAGCTGGACACCTCCCCCTCCCCCTCCGCTCAGGTCGTCGGGCTGAAGCAC
GCTCTGTCATCCGAAAACTCCAGGGATGAGGGCCGAGCCCTGGTGCACCAGCTCTCCAATGAGAGCAGGC
TGTCTATCACCGACTCCCTTTCTGAGTTCTTCGATGCCCAGGAAGTTCTGCTGTCTCCTAGCTCTTCAGA
AAACGAGATTTCTGATGATGATTCATACGTCAGTGACATAAGTGATAATCTGTCCTTAGACAACCTCAGT
AATGATTTAGACAATGAAAGACAGACCTTGGGGCCTGTTCTCGAGAGTAGTGGGGAAGCCAGATCCAAAA
GGAGGACCAGCTTGCCGGCACCGGGTCCCAACACCAGTAGCGTTAGCTTGTGGAGCATCCTGCGCAACAA
CATTGGGAAGGACCTGTCCAAGGTGGCCATGCCGGTGGAGCTGAACGAGCCCCTGAACACGCTGCAGCGG
CTCTGCGAGGAGCTGGAATACAGCGAGCTTCTGGACAAGGCCTCGAGAATCCCCAGTCCCCTGGAGAGGA
TGGTGTACGTAGCAGCCTTCGCCATCTCCGCATATGCATCCAGCTACTTCCGAGCAGGCAGCAAGCCGTT
TAACCCGGTCCTTGGAGAAACGTACGAATGCATCCGACAGGACAAGGGTTTCCAGTTTTTTGCCGAGCAG
GTCAGCCACCACCCACCTATCTCCGCCTGCCACGCTGAGTCCGGAAACTTTGTTTTCTGGCAAGATGTGA
GATGGAAAAATAAATTCTGGGGTAAATCCATGGAAATTGTCCCGATTGGCACAACCCACGTGACTCTGCC
TGCTTTTGGAGATCACTTTGAGTGGAACAAAGTAACCTCCTGCATCCACAACATCTTGAGCGGACAGCGA
TGGATCGAGCACTATGGCGAAATTGACATCAAGAACCTGAATGACGACTCCTGCCACTGCAAAGTGAACT
TCATAAAGGCTAAGTACTGGAGCACTAATGCCCACGAGATCGAAGGCACGGTGTTCGACCGCAGTGGGAA
GGCTGTGCACCGGCTGTTTGGGAAATGGCACGAGAGCATCTACTGTGGCGGCGCCTCCTCTTCCACATGC
GTCTGGAGAGCAAATCCCATGCCGAAAGGCTATGAGCAGTATTATGGCTTCACACAGTTTGCACTAGAGT
TAAATGAAATGGATCCTTTGTCAAGGTCTTTATTACCACCCACCGACACTCGGTTTAGACCAGATCAAAG
GCTTCTAGAGGAAGGGAACATAGAGGAGGCTGAGGTTCAGAAGCAGAGGATCGAGAAGCTGCAGAGAGAG
CGGCGGAGGGTCTTGGAGGAGAACGGTGTGGAGCACCAGCCCCGGTTTTTCAGGAAATCCAGTGACGATG
CTTGGGTAAGCAATGGGACCTACCTGGAACTGAGGAAAGACCTTGGCTTTTCCAAGTTGGACCATCCCGT
CTTATGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210802 representing BC086485
Red=Cloning site Green=Tags(s)

MSDEKNLGVSQKLVSPSRSTSSCSSKQGSRQDSWEVVEGLRGEMTYTQEPPVQKGFLLKKRKWPLKGWHK
RFFCLEKGILKYAKSQADIEREKLHGCIDVGLSVMSVKKSSKCIDLDTEEHIYHLKVKSEELFDEWVSKL
RHHRMYRQNEIAMFPRDVNHFFSGSSVTDSAPGVFESVSSRKRSSLSKQNSFPPGSNLSFSCGGDTRVPF
WLQSSEDMEKCSKDLAHCHAYLLEMSQLLESMDVLHRTYSAPAINAIQVPKPFSGPVRLHSSNPNLSTLD
FGEEKSYSDGSEASSEFSKMQEDLCHVAHKVYFALRSAFNSISVEREKLKQLMELDTSPSPSAQVVGLKH
ALSSENSRDEGRALVHQLSNESRLSITDSLSEFFDAQEVLLSPSSSENEISDDDSYVSDISDNLSLDNLS
NDLDNERQTLGPVLESSGEARSKRRTSLPAPGPNTSSVSLWSILRNNIGKDLSKVAMPVELNEPLNTLQR
LCEELEYSELLDKASRIPSPLERMVYVAAFAISAYASSYFRAGSKPFNPVLGETYECIRQDKGFQFFAEQ
VSHHPPISACHAESGNFVFWQDVRWKNKFWGKSMEIVPIGTTHVTLPAFGDHFEWNKVTSCIHNILSGQR
WIEHYGEIDIKNLNDDSCHCKVNFIKAKYWSTNAHEIEGTVFDRSGKAVHRLFGKWHESIYCGGASSSTC
VWRANPMPKGYEQYYGFTQFALELNEMDPLSRSLLPPTDTRFRPDQRLLEEGNIEEAEVQKQRIEKLQRE
RRRVLEENGVEHQPRFFRKSSDDAWVSNGTYLELRKDLGFSKLDHPVLW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC086485
ORF Size 2457 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC086485.1
RefSeq Size 3233 bp
RefSeq ORF 2459 bp
Locus ID 71720
Cytogenetics 6 B2.3
MW 118.5 kDa
Summary Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and cholesterol.UniProtKB/Swiss-Prot Function
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"BC086485" in other vectors (4)
SKU Description Size Price
MC220828 Osbpl3 (untagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992), (10ug) 10 ug
$826.00
MG210802 Osbpl3 (tGFP-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) 10 ug
$950.00
MR210802L3 Lenti ORF clone of Osbpl3 (Myc-DDK-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) 10 ug
$1,050.00
MR210802L4 Lenti ORF clone of Osbpl3 (mGFP-tagged) - Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992) 10 ug
$1,050.00

Plasmid Map for MR210802

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210802
Circular map for MR210802

file-alt Citations

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