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Tmc6 (BC004840) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210776
Tmc6 (Myc-DDK-tagged) - Mouse transmembrane channel-like gene family 6 (cDNA clone MGC:6819 IMAGE:2648849)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $742.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmc6
Synonyms D11Ertd204e; EVER1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210776 representing BC004840
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCAGTCACTGGCTTTAGCCCTCGATGTCCCGGAGACCACGGGAGACGAAGGCCTGGAGCCCAGCC
CCTATGAAGAAAGTGAAGTCCATGACTCCTTCCACCAGCTCATTCAAGAGCAAAGCCTTCGGGTGGCGGA
GGAGGGGCTGGAGCTACTGCCCTTGGGTCTAGGCAGAGGTGACCAGACCCTCCCAGGACTTGAAGGTGCT
CCTGCCCTTAGCTCGGCCACTCTCCGCATCCTGGCCAGCATGCCCAGCCGTACCATTGGTCGCAGCCGTG
GGGCCATCATCTCCCAATACTACAACCGCACAGTGAGGCTTCGGCGCAGGAGCAGCCGGCCCTTGCTGGG
CAATGTGGTGCCCTCTGCCCGGCCCAGCCTTCGCCTGTATGACCTGGAGCTGGACTCCACAATCTTGGAG
GAGGATGAGAAGCGGAGTCTGCTAGTAAAGGAGCTTCAAGGTCTGTCGGCGGCCCAGAGGGACCACATGG
TGCGGAACATGCCCTTGAGTCTGGGTGAGAAGCGCTGTCTTCGAGAGAAAAGTTGGAGCCCAAAGGGAAA
GCGGCGGCACCTGCAGGGTCGAAGCGGGGCCTTCTCCTGCTGTAGCCGGCTCAGATACACCTGCATGCTG
GCTCTTCATAGCCTGGGGCTGGCACTGCTCTCAGGCCTGTATGCTGCCAGGCCGTGGCGCTATGCTCTGA
AGCAGATCGGTGGCCAGTTTGGCTCCAGCGTCCTCTCCTACTTCCTCTTCCTCAAGACCCTGTTGGCCTT
CAACGCGCTGATGTTGCTGCCTTTGCTGGCCTTCCTCGTGGGTGTTCAGGCTGCCTTTCCACCTGACCCA
GCAGGCCCCGTGCCTACGTTTTCTGGTCTGGAACTCCTCACAGGCGGGGGCCGGTTCACACACACAGTTA
TGTACTACGGCTACTACAGTAACAGCACGCTGAGCCCGTCATGTGACGCCCCTCGGGAAGGTGGCCAGTG
CAGTCCCAGGCTGGGCAGCCTGCCCTATAACATGCCGCTGGCCTACCTCTTCACAATGGGGGCCACCTTC
TTCCTTACCTGCATCATTCTGGTATACAGCATGTCCCACTCCTTTGGGGAGAGCTACCGGGTTGGCAGTA
CCAAGGGTATCCATGCCCTTACGGTCTTCTGCTCCTGGGACTACAAGGTGACACAGAAGAGGGCTTCCCG
TGTCCAACAGGACAGCATCTGCACTCAGCTGAAGGAGCTGTTGGCTGAATGGCACCTTCGAAAGCGCCCT
CGGAGTGTGTGCGGGCAGCTGAGGCAGGTCGTCGTTCTAGGTCTGGGGTGGCTGCTGTGTCTGGGCTCCA
CAATGGGCTGCACGGTGGCTGTCCTCACCTTCTCAGAGGTAATGATTCAGAGACCTGCTTCTGGTGGCCA
GGGGGTGGAGGCGTTGGCCTTGCCCCTGGTGGTCAGTGTCCTTAACCTGGGTGCCTCCTACCTGTTCCGT
GGTCTAGCCACTCTGGAGCGACATGACTCCCCTGTGTTGGAGGTATACATGGCCATCTGCAGGAACCTCA
TCCTGAAGATGGCCGTCCTGGGTGTGCTTTGCTATCACTGGCTGGGCCGCAGGGTGGCCACCCTGCAGGG
TCAGTGCTGGGAGGACTTTGTGGGCCAGGAGCTGTACCGCTTCATGGTCGTGGATTTCATCTTCATGCTC
TTGGACTCCCTTTTTGGAGAGTTGGTGTGGAGGCTCATCTCAGAGAAGAAGCTCAAGAGGGGGCAGAAGC
CTGAGTTTGACATTGCCAGGAATGTGCTGGACCTGATTTATGGACAGACACTGACCTGGCTGGGCGTCCT
GTTCTCACCGCTCCTGCCTGCAGTACAGATTCTCCGGCTGCTCTTCCTTTTCCACATCAAGAAGGCCAGC
CTGATGGCCAACTGCCAGGCACCACGCCGACCCTGGCTGGCCTCGCACATGAGCACTGTCTTCCTCACCT
TGCTCTGCTTCCCGTCGTTCCTGGGCGCTGCTGTTTTCCTCTGCTATGCTGTCTGGCAGGTGAGGCCCTC
GAGCACTTGTGGCCCTTTCCGGACTCTGAACACCATGTATGAAGCAGGCACGGTCTGGGTGCGTCGCCTG
GAGCATGCAGGCTCCGGAGCCTCCTGGCTGCCCTGGCTGCACCACTTCCTGGTGGAGAACACTTTCTTCC
TCTTCCTGGCTTCAGCCCTGCTGCTGGCTGTCATCTACTTCAACATCCAGGTGGTGAAAGGACAACGGAA
GGTCATCTGCCTGCTCAAGGAGCAGATCCGGAATGAAGGAGAGGACAAGATCTTCCTGATCAACAAGCTT
CACTCTGTTTACGAGGAGGAGGGAAGGAGCAGGCCTGGCAGAACCCAGGATGCTACTGAACCACCTGCCT
GGCATGAGGATGGAGGGGACCAGAAGGAACCCTGTAACCCCCGGTCACCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210776 representing BC004840
Red=Cloning site Green=Tags(s)

MAQSLALALDVPETTGDEGLEPSPYEESEVHDSFHQLIQEQSLRVAEEGLELLPLGLGRGDQTLPGLEGA
PALSSATLRILASMPSRTIGRSRGAIISQYYNRTVRLRRRSSRPLLGNVVPSARPSLRLYDLELDSTILE
EDEKRSLLVKELQGLSAAQRDHMVRNMPLSLGEKRCLREKSWSPKGKRRHLQGRSGAFSCCSRLRYTCML
ALHSLGLALLSGLYAARPWRYALKQIGGQFGSSVLSYFLFLKTLLAFNALMLLPLLAFLVGVQAAFPPDP
AGPVPTFSGLELLTGGGRFTHTVMYYGYYSNSTLSPSCDAPREGGQCSPRLGSLPYNMPLAYLFTMGATF
FLTCIILVYSMSHSFGESYRVGSTKGIHALTVFCSWDYKVTQKRASRVQQDSICTQLKELLAEWHLRKRP
RSVCGQLRQVVVLGLGWLLCLGSTMGCTVAVLTFSEVMIQRPASGGQGVEALALPLVVSVLNLGASYLFR
GLATLERHDSPVLEVYMAICRNLILKMAVLGVLCYHWLGRRVATLQGQCWEDFVGQELYRFMVVDFIFML
LDSLFGELVWRLISEKKLKRGQKPEFDIARNVLDLIYGQTLTWLGVLFSPLLPAVQILRLLFLFHIKKAS
LMANCQAPRRPWLASHMSTVFLTLLCFPSFLGAAVFLCYAVWQVRPSSTCGPFRTLNTMYEAGTVWVRRL
EHAGSGASWLPWLHHFLVENTFFLFLASALLLAVIYFNIQVVKGQRKVICLLKEQIRNEGEDKIFLINKL
HSVYEEEGRSRPGRTQDATEPPAWHEDGGDQKEPCNPRSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC004840
ORF Size 2430 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC004840.1
RefSeq Size 2848 bp
RefSeq ORF 2432 bp
Locus ID 217353
Cytogenetics 11 82.96 cM
MW 104.4 kDa
Summary Probable ion channel.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC004840" in other vectors (4)
SKU Description Size Price
MC200459 Tmc6 (untagged) - Mouse transmembrane channel-like gene family 6 (cDNA clone MGC:6819 IMAGE:2648849), (10ug) 10 ug
$743.00
MG210776 Tmc6 (tGFP-tagged) - Mouse transmembrane channel-like gene family 6 (cDNA clone MGC:6819 IMAGE:2648849) 10 ug
$789.00 $942.00
MR210776L3 Lenti ORF clone of Tmc6 (Myc-DDK-tagged) - Mouse transmembrane channel-like gene family 6 (cDNA clone MGC:6819 IMAGE:2648849) 10 ug
$1,042.00
MR210776L4 Lenti ORF clone of Tmc6 (mGFP-tagged) - Mouse transmembrane channel-like gene family 6 (cDNA clone MGC:6819 IMAGE:2648849) 10 ug
$1,042.00

Plasmid Map for MR210776

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210776
Circular map for MR210776

file-alt Citations

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