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Tril (NM_025817) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210772
Tril (Myc-DDK-tagged) - Mouse TLR4 interactor with leucine-rich repeats (Tril)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $742.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tril
Synonyms 1200009O22Rik; AI316813; mKIAA0644
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210772 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGTGTGGGTGCCGTGCGCTTCTGGCTCGTGGTGTGCGGCTGCCTGGCGTTCCCGCCGCGGGCCG
AGTCCGTGTGCCCGGAGCGCTGCGACTGCCAGCACCCCCAACATCTCCTATGCACCAACAGGGGACTTCG
CGCTGTACCCAAGACCAGCTCGCTGCCTAGTCCCCAGGACGTGCTCACCTACAGCCTAGGCGGCAACTTC
ATCACCAACATCACCGCCTTCGACTTCCACCGTCTGGGGCAGCTCAGACGGCTGGATCTGCAGTACAACC
AGATCCGTTCTCTCCACCCCAAGACCTTCGAGAAGCTCTCACGACTAGAGGAGCTGTACCTGGGGAACAA
CCTCTTGCAGGCGCTCGTTCCTGGCACGTTGGCTCCGCTGCGCAAGTTGCGCATCCTTTACGCCAACGGC
AACGAGATTGGACGCCTGAGTCGCGGGTCCTTCGAGGGCCTGGAGAGTTTGGTCAAGCTACGGCTGGACG
GGAACGTGCTGGGAGCGCTGCCGGACGCGGTCTTCGCTCCCCTGGGCAACCTGCTCTACCTACACCTGGA
ATCTAACCGGATCCGTTTTTTGGGCAAGAACGCCTTCAGCCAGCTGGGTAAGCTTCGATTCCTCAATCTC
TCGGCCAACGAGCTGCAACCCTCTCTGCGCCACGCGGCCACCTTTGTCCCTCTGCGCTCCCTCTCTACTC
TCATCCTCTCAGCCAACAGCCTACAGCACCTCGGCCCCCGCGTCTTCCAGCACCTGCCACGCCTAGGCCT
GCTCTCCCTCAGTGGAAACCAGCTCACGCACCTCGCCCCAGAGGCCTTTTGGGGACTGGAGGCCCTACGT
GAGCTGCGCCTGGAAGGTAACCGGCTGAACCAGCTCCCCCTGACACTGCTGGAGCCTCTGCACAGCTTGG
AGGCACTAGACCTGAGTGGCAATGAGCTTTCTGCTCTGCACCCTGCCACCTTTGGCCACCAGGGCCGGCT
CCGTGAGCTCAGCCTGCGCGACAACGCGCTCAGCGCCCTTTCTGGGGACATCTTTGCCGCCAGTCCGGCT
CTCTACCGGCTGGATCTAGACGGCAATGGTTGGACCTGCGACTGCCGGTTGCGGGGTCTGAAACGCTGGA
TGGGCAACTGGCATTCTCAGGGTCGCCTCCTTACGGTCTTCGTGCAGTGTCGCCACCCCCCGGCCCTGCG
GGGCAAGTACCTGGATTACCTGGATGACCAACTGCTGCAGAATGGGTCTTGCGTGGATCCCTCACCTTCC
CCTACAGCAGGCAGTAGGCAGTGGCCGCTACCCACATCGTCAGAAGAGGGGATGACGCCCCCTGCAGGCC
TCTCGCAGGAGCTACCACTGCAGCCGCAGCCGCAGCCCCAGCAGCGGGGAAGACTTCTACCAGGAGTGGC
CTGGGGTGGGGCTGCCAAGGAACTGGTGGGCAACCGCAGCGCATTAAGGTTGAGCCGGCGGGGACCAGGT
CCCCATCAGGGCCCCTCTGCTGCGGCTCCGGGCTCTGCCCCACAGTCCTTGGACCTGCACGAGAAGCCGG
GGAGAGGACGTCACACCCGAGCCAATCTCTCCCAGACGGAGCCCACCCCGACGTCTGAGCCCGCCTCTGG
GACACCATCTGCCAGAGACAGCTGGCAGCGCGCAGCAAAGCAGCGCCTAGCGTCCGAGCAGCAAGAGAGC
GCAGTCCAGTCCGTTAGTGGGGTTGGTTTGCCACCTCTGGTGTCTGACCCCTGTGACTTCAACAAATTCA
TTCTGTGCAACCTGACAGTAGAGGCGGTGAGCGCCAACAGCGCCTCGGTGCGCTGGGCGGTTCGCGAACA
CCGCAGTCCCCGGCCGCAGGGCGGCGCACGCTTCCGCCTGCTTTTCGACCGCTTTGGCCAGCAGCCCAAG
TTCCAGCGCTTCGTCTACCTGCCGGAGCGCAGCGACTCGGCCACGCTGCACGAGCTGCGCGGAGACACCC
CCTACCTAGTATGCGTGGAGGGTGTGCTCGGGGGTCGCGTTTGCCCCGTGGCCCCCCGAGACCACTGCGC
AGGGTTAGTAACCCTGCCAGAGGCAGGAGGTCGAGGTGGCGTCGACTACCAGCTCCTGACCTTGGTCTTG
CTGGCTGTCAACGCGCTTCTGGTGCTCCTGGCACTGGCAGCCTGGGGATCGCGATGGCTGCGGAGGAAGC
TGCGTGCCAGGCGGAAGGGAGGGGCCCCGGTCCACGTTCGCCACATGTACTCCACCCGACGGCCACTGCG
CTCCATGGGCACTGGTGTGTCCGCGGACTTCTCAGGCTTCCAGTCGCACCGTCCCCGCACCACCGTGTGC
GCGCTCAGCGAGGCCGACCTCATTGAGTTCCCCTGCGACCGCTTCATGGACAGCACCGGCGGTGGTACCA
GTGGCAGCTTGAGGCGGGAGGATCACCTCTTGCAAAGATTCGCTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210772 protein sequence
Red=Cloning site Green=Tags(s)

MEGVGAVRFWLVVCGCLAFPPRAESVCPERCDCQHPQHLLCTNRGLRAVPKTSSLPSPQDVLTYSLGGNF
ITNITAFDFHRLGQLRRLDLQYNQIRSLHPKTFEKLSRLEELYLGNNLLQALVPGTLAPLRKLRILYANG
NEIGRLSRGSFEGLESLVKLRLDGNVLGALPDAVFAPLGNLLYLHLESNRIRFLGKNAFSQLGKLRFLNL
SANELQPSLRHAATFVPLRSLSTLILSANSLQHLGPRVFQHLPRLGLLSLSGNQLTHLAPEAFWGLEALR
ELRLEGNRLNQLPLTLLEPLHSLEALDLSGNELSALHPATFGHQGRLRELSLRDNALSALSGDIFAASPA
LYRLDLDGNGWTCDCRLRGLKRWMGNWHSQGRLLTVFVQCRHPPALRGKYLDYLDDQLLQNGSCVDPSPS
PTAGSRQWPLPTSSEEGMTPPAGLSQELPLQPQPQPQQRGRLLPGVAWGGAAKELVGNRSALRLSRRGPG
PHQGPSAAAPGSAPQSLDLHEKPGRGRHTRANLSQTEPTPTSEPASGTPSARDSWQRAAKQRLASEQQES
AVQSVSGVGLPPLVSDPCDFNKFILCNLTVEAVSANSASVRWAVREHRSPRPQGGARFRLLFDRFGQQPK
FQRFVYLPERSDSATLHELRGDTPYLVCVEGVLGGRVCPVAPRDHCAGLVTLPEAGGRGGVDYQLLTLVL
LAVNALLVLLALAAWGSRWLRRKLRARRKGGAPVHVRHMYSTRRPLRSMGTGVSADFSGFQSHRPRTTVC
ALSEADLIEFPCDRFMDSTGGGTSGSLRREDHLLQRFAD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025817
ORF Size 2427 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025817.4, NP_080093.1
RefSeq Size 5377 bp
RefSeq ORF 2430 bp
Locus ID 66873
UniProt ID Q9DBY4
Cytogenetics 6 B3
MW 88.8 kDa
Summary Component of the TLR4 signaling complex. Mediate the innate immune response to bacterial lipopolysaccharide (LPS) leading to cytokine secretion (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025817" in other vectors (4)
SKU Description Size Price
MC221762 Tril (untagged) - Mouse TLR4 interactor with leucine-rich repeats (Tril), (10ug) 10 ug
$816.00
MG210772 Tril (tGFP-tagged) - Mouse RIKEN cDNA 1200009O22 gene (1200009O22Rik) 10 ug
$942.00
MR210772L3 Lenti ORF clone of Tril (Myc-DDK-tagged) - Mouse TLR4 interactor with leucine-rich repeats (Tril) 10 ug
$1,042.00
MR210772L4 Lenti ORF clone of Tril (mGFP-tagged) - Mouse TLR4 interactor with leucine-rich repeats (Tril) 10 ug
$1,042.00

Plasmid Map for MR210772

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210772
Circular map for MR210772

file-alt Citations

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