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Mcm8 (NM_025676) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210756
Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $738.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mcm8
Synonyms 5730432L01Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210756 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGGTGCTTATAGAGGCAGAGGTTTTGGACGAGGAAGATTCCAAAGCTGGAAAAGAGGAAGAGGTG
GTGGGAACTTCTCAGGAAGGTGGAGAGAAAGAGAAAACAGAGTTGACCTGAATGAAGCTTCAGGAAAGCA
CGCTTCTGTTTACAGTAATAACTCTCCCTTTATTGAGAAGATTCAAGCATTTGAGAAATTTTTCACAAGG
CATATCGACTTATACGATAAGGATGAAATAGAAAGAAAAGGAAGCATTTTGGTGGATTTTAAAGAACTGA
CAAAAGCTGATGAAATAACTAACTTGATACCCGATATAGAAAACGCACTAAGAGATGCCCCTGAGAAAAC
ACTGGCGTGTATGGGGCTGGCAATACATCAGGTATTAACTAAGGACCTTGAAAGGCATGCCGCCGAATTA
CAAGCTCAAGAGGGATTGTCTAACGGTGGAGAGACAATGGTAAATGTACCACATATTTATGCAAGAGTGT
ACAACTATGAGCCCTTGACACACCTCAAGAATATCCGAGCAACTTGCTATGGGAAATACATCTCTATACG
AGGGACTGTGGTCCGGGTCAGCAACATAAAGCCTCTTTGTACCAACATGGCTTTTCAGTGCGCTGCATGT
GGAGAGATTCAGAGCTTTCCTCTGCCAGATGGAAAATACACCCTTCCTACAAAGTGTCCTGTGCCTGCGT
GCCGAGGGAGGTCATTTGCTCCACTGCGCAGCTCTCCTCTCACAGTTACACTGGACTGGCAGTTGATCAA
AATCCAGGAGCTGATGTCTGATGCACAGAGAGAAGCTGGTCGGATCCCTCGGACGATAGAATGTGAACTT
GTTCACGATCTTGTAGATAGTTGTGTCCCAGGAGATACAGTGACTGTTACTGGAATTGTCAAAGTCTCCA
ATTCTGAAGAAGGTTCTCGAAATAAGAATGATAAGTGCATGTTCCTTTTGTACATTGAGGCAAATTCTGT
TAGCAACAGCAAGGGGCCGAAAGCACAGACTGCAGAAGATGGTTGCAAGCATGGGACACTGATGGAGTTC
TCCCTTAAAGACCTCTATGCCATCCGAGAGATCCAGGCTGAAGAGAACCTGCTCAAGCTCGTTGTCAACT
CACTCTGTCCTGTCATTTTTGGTCATGAACTCGTCAAAGCAGGTCTGACGTTAGCACTCTTTGGCGGTAG
CCAGAAGTACGCAGATGACAAAAACAGAATTCCCATTCGAGGAGACCCACATGTCCTGATTGTTGGAGAT
CCAGGCTTGGGGAAGAGTCAGATGCTACAGGCAGCATGCAACGTGGCGCCACGTGGTGTGTATGTTTGTG
GAAATACCACCACCAGCTCTGGTCTCACTGTGACTCTTTCAAAGGACAGTTCCTCTGGAGATTTTGCTTT
GGAAGCTGGTGCCCTTGTACTTGGTGACCAAGGCATCTGTGGAATAGATGAATTTGATAAAATGGGGAAC
CAACATCAAGCCCTGTTAGAAGCCATGGAACAGCAGAGTATTAGCCTTGCGAAGGCTGGGGTAGTTTGCA
GCCTCCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTCGGAGGACACTACAATAAAGCCAGAAC
GGTTTCTGAGAATTTAAAGATGGGGAGTGCCCTACTGTCCAGATTCGATTTGGTCTTTATCTTGTTAGAT
ACGCCAAATGAACAGCATGACCACTTACTTTCTGAACATGTGATCGCGATAAGAGCTGGGAAGCAGAAAG
CGGTTAGCAGTGCCACAGTCACTCGTGTGCTCAGTCAAGACTCAAATACTTCTGTACTTGAAGTGGTTTC
TGAGAAACCATTATCAGAAAGACTAAAGGTGGCTCCTGGAGAACAGACAGATCCGATTCCACATCAGCTG
TTGAGGAAGTACATTGGCTATGCACGGCAGTATGTCCACCCAAGGTTATCTACGGACGCTGCTCAGGCCC
TTCAGGATTTCTACCTGGAGCTCCGCAAGCAGAGCCAGCGCGTGGGCAGCTCACCCATCACCACTCGGCA
GCTGGAGTCTTTGATCCGTCTGACAGAGGCACGGGCAAGGTTAGAATTGAGAGAGGAAGCAACTAGAGAA
GATGCTGAAGATATAATTGAAATTATGAAGCATAGCATGCTAGGAACTTACTCAGATGAATTCGGAAACC
TGGACTTTGAGCGATCCCAGCATGGCTCTGGGATGAGCAACAGGTCAACAGCAAAAAGATTTATTTCTGC
TCTCAACAGCATTGCTGAAAGAACTTATAACAACATATTTCAATATCATCAACTTCGTCAGATTGCTAAA
GAACTAAACATTCAGGTTGCCGATTTTGAAAACTTCATTGGATCACTGAATGACCAAGGTTATCTCTTGA
AGAAAGGCCCAAAGATTTACCAACTTCAAACTATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210756 protein sequence
Red=Cloning site Green=Tags(s)

MSGAYRGRGFGRGRFQSWKRGRGGGNFSGRWRERENRVDLNEASGKHASVYSNNSPFIEKIQAFEKFFTR
HIDLYDKDEIERKGSILVDFKELTKADEITNLIPDIENALRDAPEKTLACMGLAIHQVLTKDLERHAAEL
QAQEGLSNGGETMVNVPHIYARVYNYEPLTHLKNIRATCYGKYISIRGTVVRVSNIKPLCTNMAFQCAAC
GEIQSFPLPDGKYTLPTKCPVPACRGRSFAPLRSSPLTVTLDWQLIKIQELMSDAQREAGRIPRTIECEL
VHDLVDSCVPGDTVTVTGIVKVSNSEEGSRNKNDKCMFLLYIEANSVSNSKGPKAQTAEDGCKHGTLMEF
SLKDLYAIREIQAEENLLKLVVNSLCPVIFGHELVKAGLTLALFGGSQKYADDKNRIPIRGDPHVLIVGD
PGLGKSQMLQAACNVAPRGVYVCGNTTTSSGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGN
QHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKARTVSENLKMGSALLSRFDLVFILLD
TPNEQHDHLLSEHVIAIRAGKQKAVSSATVTRVLSQDSNTSVLEVVSEKPLSERLKVAPGEQTDPIPHQL
LRKYIGYARQYVHPRLSTDAAQALQDFYLELRKQSQRVGSSPITTRQLESLIRLTEARARLELREEATRE
DAEDIIEIMKHSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNSIAERTYNNIFQYHQLRQIAK
ELNIQVADFENFIGSLNDQGYLLKKGPKIYQLQTM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025676
ORF Size 2415 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025676.2
RefSeq Size 3397 bp
RefSeq ORF 2418 bp
Locus ID 66634
UniProt ID Q9CWV1
Cytogenetics 2 F2
MW 89.1 kDa
Summary Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC) (By similarity). Probably by regulating HR, plays a key role during gametogenesis (PubMed:22771120). Stabilizes MCM9 protein (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025676" in other vectors (4)
SKU Description Size Price
MC221745 Mcm8 (untagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8), (10ug) 10 ug
$812.00
MG210756 Mcm8 (tGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) 10 ug
$938.00
MR210756L3 Lenti ORF clone of Mcm8 (Myc-DDK-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) 10 ug
$1,038.00
MR210756L4 Lenti ORF clone of Mcm8 (mGFP-tagged) - Mouse minichromosome maintenance deficient 8 (S. cerevisiae) (Mcm8) 10 ug
$1,038.00

Plasmid Map for MR210756

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210756
Circular map for MR210756

file-alt Citations

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