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Ppargc1a (NM_008904) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210710
Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK DDK w/ Puro
AAV Particle:
DDK
$589.00 $1,094.00
2 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
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AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
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Other products for "Ppargc1a or NM_008904"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppargc1a
Synonyms A830037N07Rik; Gm11133; Pgc; PGC-1; Pgc-1alpha; Pgc1; Pgco1; PPARGC-1-alpha; Ppargc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210710 representing NM_008904
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTGGGACATGTGCAGCCAAGACTCTGTATGGAGTGACATAGAGTGTGCTGCTCTGGTTGGTGAGG
ACCAGCCTCTTTGCCCAGATCTTCCTGAACTTGACCTTTCTGAACTTGATGTGAATGACTTGGATACAGA
CAGCTTTCTGGGTGGATTGAAGTGGTGTAGCGACCAATCGGAAATCATATCCAACCAGTACAACAATGAG
CCTGCGAACATATTTGAGAAGATAGATGAAGAGAATGAGGCAAACTTGCTAGCGGTCCTCACAGAGACAC
TGGACAGTCTCCCCGTGGATGAAGACGGATTGCCCTCATTTGATGCACTGACAGATGGAGCCGTGACCAC
TGACAACGAGGCCAGTCCTTCCTCCATGCCTGACGGCACCCCTCCCCCTCAGGAGGCAGAAGAGCCGTCT
CTACTTAAGAAGCTCTTACTGGCACCAGCCAACACTCAGCTCAGCTACAATGAATGCAGCGGTCTTAGCA
CTCAGAACCATGCAGCAAACCACACCCACAGGATCAGAACAAACCCTGCCATTGTTAAGACCGAGAATTC
ATGGAGCAATAAAGCGAAGAGCATTTGTCAACAGCAAAAGCCACAAAGACGTCCCTGCTCAGAGCTTCTC
AAGTATCTGACCACAAACGATGACCCTCCTCACACCAAACCCACAGAAAACAGGAACAGCAGCAGAGACA
AATGTGCTTCCAAAAAGAAGTCCCATACACAACCGCAGTCGCAACATGCTCAAGCCAAACCAACAACTTT
ATCTCTTCCTCTGACCCCAGAGTCACCAAATGACCCCAAGGGTTCCCCATTTGAGAACAAGACTATTGAG
CGAACCTTAAGTGTGGAACTCTCTGGAACTGCAGGCCTAACTCCTCCCACAACTCCTCCTCATAAAGCCA
ACCAAGATAACCCTTTCAAGGCTTCGCCAAAGCTGAAGCCCTCTTGCAAGACCGTGGTGCCACCGCCAAC
CAAGAGGGCCCGGTACAGCGAGTGTTCTGGTACCCAAGGCAGCCACTCCACCAAGAAAGGGCCCGAGCAA
TCTGAGTTGTACGCACAACTCAGCAAGTCCTCAGGGCTCAGCCGAGGACACGAGGAAAGGAAGACTAAAC
GGCCCAGTCTTCGGCTGTTTGGTGACCATGACTATTGTCAGTCACTCAATTCCAAAACGGATATACTCAT
TAACATATCACAGGAGCTCCAAGACTCTAGACAACTAGACTTCAAAGATGCCTCCTGTGACTGGCAGGGG
CACATCTGTTCTTCCACAGATTCAGGCCAGTGCTACCTGAGAGAGACTTTGGAGGCCAGCAAGCAGGTCT
CTCCTTGCAGCACCAGAAAACAGCTCCAAGACCAGGAAATCCGAGCGGAGCTGAACAAGCACTTCGGTCA
TCCCTGTCAAGCTGTGTTTGACGACAAATCAGACAAGACCAGTGAACTAAGGGATGGCGACTTCAGTAAT
GAACAATTCTCCAAACTACCTGTGTTTATAAATTCAGGACTAGCCATGGATGGCCTATTTGATGACAGTG
AAGATGAAAGTGATAAACTGAGCTACCCTTGGGATGGCACGCAGCCCTATTCATTGTTCGATGTGTCGCC
TTCTTGCTCTTCCTTTAACTCTCCGTGTCGAGACTCAGTGTCACCACCGAAATCCTTATTTTCTCAAAGA
CCCCAAAGGATGCGCTCTCGTTCAAGATCCTTTTCTCGACACAGGTCGTGTTCCCGATCACCATATTCCA
GGTCAAGATTAAGGTCCCCAGGCAGTAGATCCTCTTCAAGATCCTGTTACTACTATGAATCAAGCCACTA
CAGACACCGCACACACCGCAATTCTCCCTTGTATGTGAGATCACGTTCAAGGTCACCCTACAGCTGTAGG
CCCAGGTACGACAGCTATGAAGCCTATGAGCACGAAAGGCTCAAGAGGGATGAATACCGCAAAGAGCACG
AGAAGCGGGAGTCTGAAAGGGCCAAGCAGAGAGAGAGGCAGAAGCAGAAAGCAATTGAAGAGCGCCGTGT
GATTTACGTTGGTAAAATCAGACCTGACACAACGCGGACAGAATTGAGAGACCGCTTTGAAGTTTTTGGT
GAAATTGAGGAATGCACCGTAAATCTGCGGGATGATGGAGACAGCTATGGTTTCATCACCTACCGTTACA
CCTGTGACGCTTTCGCTGCTCTTGAGAATGGATATACTTTACGCAGGTCGAACGAAACTGACTTCGAGCT
GTACTTTTGTGGACGGAAGCAATTTTTCAAGTCTAACTATGCAGACCTAGATACAAACTCAGACGATTTT
GACCCTGCTTCCACCAAGAGCAAGTATGACTCTCTGGATTTTGATAGTTTACTGAAGGAAGCTCAGAGAA
GCTTGCGCAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210710 representing NM_008904
Red=Cloning site Green=Tags(s)

MAWDMCSQDSVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNE
PANIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGAVTTDNEASPSSMPDGTPPPQEAEEPS
LLKKLLLAPANTQLSYNECSGLSTQNHAANHTHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELL
KYLTTNDDPPHTKPTENRNSSRDKCASKKKSHTQPQSQHAQAKPTTLSLPLTPESPNDPKGSPFENKTIE
RTLSVELSGTAGLTPPTTPPHKANQDNPFKASPKLKPSCKTVVPPPTKRARYSECSGTQGSHSTKKGPEQ
SELYAQLSKSSGLSRGHEERKTKRPSLRLFGDHDYCQSLNSKTDILINISQELQDSRQLDFKDASCDWQG
HICSSTDSGQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPCQAVFDDKSDKTSELRDGDFSN
EQFSKLPVFINSGLAMDGLFDDSEDESDKLSYPWDGTQPYSLFDVSPSCSSFNSPCRDSVSPPKSLFSQR
PQRMRSRSRSFSRHRSCSRSPYSRSRLRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSCR
PRYDSYEAYEHERLKRDEYRKEHEKRESERAKQRERQKQKAIEERRVIYVGKIRPDTTRTELRDRFEVFG
EIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDTNSDDF
DPASTKSKYDSLDFDSLLKEAQRSLRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008904
ORF Size 2391 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008904.1, NM_008904.2, NP_032930.1
RefSeq Size 3029 bp
RefSeq ORF 2394 bp
Locus ID 19017
UniProt ID O70343
Cytogenetics 5 C1
MW 91 kDa
Summary This gene encodes a transcriptional coactivator that induces and coordinates gene expression regulating mitochondrial biogenesis, respiration, hepatic gluconeogenesis, thermogenic program in brown fat and muscle fiber-type switching. Mice lacking the encoded protein exhibit reduced thermogenic capacity, hyperactivity and resistance to diet-induced obesity. Mice lacking the encoded protein specifically in the heart exhibit peripartum cardiomyopathy. Alternative splicing results in multiple transcript variants. provided by RefSeq, Sep 2015
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"NM_008904" in other vectors (6)
SKU Description Size Price
MC204780 Ppargc1a (untagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1, (10ug) 10 ug
$1,096.00
MG210710 Ppargc1a (tGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a) 10 ug
$1,294.00
MR210710L1 Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 10 ug
$1,394.00
MR210710L2 Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 10 ug
$1,394.00
MR210710L3 Lenti ORF clone of Ppargc1a (Myc-DDK-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 10 ug
$1,394.00
MR210710L4 Lenti ORF clone of Ppargc1a (mGFP-tagged) - Mouse peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (Ppargc1a), transcript variant 1 10 ug
$1,394.00

Plasmid Map for MR210710

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210710
Circular map for MR210710

file-alt Citations

IRX5 regulates adipocyte amyloid precursor protein and mitochondrial respiration in obesity
Bjune, Haugen, Gudbrandsen et al
Int J Obes (Lond) (2018)
AI Summary: OriGene products pCMV6-hIRX5, pCDNA-mPgc-1a, and pCMV6-mApp-myk-ddk were used as overexpression plasmids to investigate the roles of IRX5, PGC-1α, and APP in adipocyte gene regulation and mitochondrial function in the study.
Snippet: Addgene plasmid #8887) [19]. The following overexpression plasmids were utilized: pCMV6-hIRX5 (Origene, Rockville, MD, USA), pCDNA-mPgc-1a (Origene), pCMV6-mApp-myk-ddk (Origene) and pCDNA-empty. ME3 and COS
Showing 1-1 of 1 papers.
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