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Vps8 (BC032214) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR210699L4
Lenti ORF clone of Vps8 (mGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,027.00
3 Weeks*
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Other products for "Vps8 or BC032214"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vps8
Synonyms mKIAA0804, MGC38232
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR210699).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC032214
ORF Size 2382 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC032214, AAH32214
RefSeq Size 3023 bp
RefSeq ORF 2384 bp
Locus ID 209018
Cytogenetics 16 B1
Summary Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).UniProtKB/Swiss-Prot Function
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"BC032214" in other vectors (4)
SKU Description Size Price
MC220673 Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693), (10ug) 10 ug
$801.00
MG210699 Vps8 (tGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$927.00
MR210699 Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$589.00 $727.00
MR210699L3 Lenti ORF clone of Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$1,027.00

Plasmid Map for MR210699L4

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Cloning scheme for MR210699L4
Circular map for MR210699L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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