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Vps8 (BC032214) Mouse Untagged Clone

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SKU
MC220673
Vps8 (untagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $801.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Vps8
Synonyms mKIAA0804, MGC38232
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC032214
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGACTTAATTGTTCATTTCCAAGATAAAAAACTGCTGGAGAATGTGGAAGCCCTCATTGTCCACA
TGGACATCACCAGCCTCGACATTCAGCAGGTGGTCCTCATGTGTTGGGAGAATCGTCTGTATGACGCCAT
GGTCTATGTCTACAACCGAGGCATGAATGAGTTTATCAGTCCGATGGAGAAGCTTTTCAAAGTCATCGCC
CCACCTCTGAATGCAGGGAAGACTCTTACAGATGAGCAAGTTGTTATGGGCAACAAGCTTCTGGTGTACA
TCAGCTGTTGCCTGGCAGGTCGTGCCTACCCTCTCGGTGACATTCCTGAAGATCTTGTTCCCTTGGTTAA
AAACCAGGTTTTTGAGTTTCTGATTCGCCTGCATTCTGTAGAAGCATCCTCCGAGGAAGAAGTCTATCCT
TATGTTCGGACTTTGCTACATTTTGACACAAGAGAATTTCTGAATGTGCTGGCACTGACCTTTGAAGACT
TTAAAAATGATAAGCAAGCTGTGGAATACCAGCAGCGCATTGTGGATATTTTATTGAAGGTTATGGTGGA
GAATTCAGATTTTACCCCGTCACAAGTGGGGTGTCTCTTTACGTTCCTCGCTCGTCAGCTCGCAAAGCCT
GACAATACCTTGTTTGTGAACAGGACCCTCTTTGATCAAGTCCTCGAGTTCCTCTGTAGCCCCGACGATG
ACTCCCGCCACTCTGAAAGACAGCAGGTCCTTTTAGAATTGCTGCAGGCCGGAGGCATAGTTCAATTTGA
AGAGAGTCGGCTCATCCGCATGGCAGAAAAGGCAGAATTCTATCAAATCTGTGAATTTATGTATGAGCGG
GAACACCAGTATGACAAGATCATTGACTGCTACCTGCACGACCCATTGAGAGAGGAAGAAGTCTTCAATT
ATATCCACAACATCTTATCTATTCCTGGTCACAGTGCTGAGGAGAAGCAGTCTGTGTGGCAGAAAGCAAT
GAATCATATGGAGGAACTTGTGTCCCTGAAGCCCTGCAAAGCCGCAGAGCTGGTGGCTACTCACTTTTCT
GAGCAGATCGAAGTGGTCATTGGACAGCTTCAGAACCAGCTTTTGCTTTTCAAATTTTTGAGGAGTCTTC
TTGATCCAAGGGAAGGTGTTCATGTCAATCAGGAGTTGCTGCAGATCCCGCCTCACATTACAGAGCAGTT
CATTGAGCTGCTGTGCCAGTTCAGTCCTGACCAAGTCATACAGACACTGCAGGTCCTTGAGTGCTACCGT
CTGGAGGAAACGATTCAGATTACACAAAAGTATCAACTCCATGAAGTCACTGCGTATCTGCTGGAGAAGA
AAGGGGATGCACACGGCGCCTTCCTGCTGCTGCTGGAGAGACTGCAGAGCAGGCTGCAAGAGATGACACG
GCAGGACGAAAATACCAAGGAGGACATCCTGCTGAAAGGTGTTGAAGATACCATGGTAGAGACAATTGCT
CTTTGCCAGAGAAATTCACAGAATTTGAATCAGCAGCAACGAGAGGCTCTATGGTTTCCACTGTTGGAGG
CAATGATGACACCACAGAAGCTGTCCAGCTCGGCTGCTGCTCCTCATCCGCACTGTGAAGCTCTGAAGTC
TTTGACCATGCAAGTCCTAAACAGCATGGCAGCGTTCATCGCCCTCCCGTCCATCCTACAGAGAATCTTG
CAGGACCCAATTTATGGAAAAGGAAAGCTTGGAGAAATCCAGGGCCTTATTCTGGGGATGCTGGACACCT
TCAACTATGAGCAAACCTTGCTAGAAACAACAGCCAGCCTCCTGAACCAAGATCTCCATTGGTCACTGTG
TAACCTGAGAGCATCAGTATCCAGAGGACTCAATCCCAAGCAGGATTACTGTTCTATATGTTTACAGCAG
TACAAAAGACGCCAAGAAATGGCTGATGAAATTATTGTCTTTAGCTGTGGCCATTTGTATCATTCATTCT
GTCTCCAAAGTAAGGAATGCACCCTAGAGGTTGAGGGTCAGACGAGATGGGCGTGTCACAAATGCAGCTC
AAGTAATAAAGCGGGCAAACTCAGTGAAAATCCTTCTGAAAACAAGAAAGGACGGATAACCTCGTCTCAG
GTAAAAATGTCGCCCTCGTATCATCAGTCCAAAGGGGATCCTCCCGCCAGGAAGGCAAACTCAGAACCTG
TTCTGGACCCACAGCAAATGCAAGCCTTTGATCAGCTCTGCCGTCTCTACAGAGGAAGTTCTAGGTTGGC
TCTCCTTACGGAGCTCTCCCAGAACCGAGGTGGCGACAGCTGCAGGCCGTTTGCTGGCCCCCAGAGTGGG
CCTGCTTTCAACAGCGTCTTCCAGAAGGAGAACTTCCAGCTGCAGCTCGCGCCTCCGCCTGTGGCTGAAG
ACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC032214
Insert Size 2385 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC032214, AAH32214
RefSeq Size 3023 bp
RefSeq ORF 2384 bp
Locus ID 209018
Cytogenetics 16 B1
Summary Plays a role in vesicle-mediated protein trafficking of the endocytic membrane transport pathway. Believed to act as a component of the putative CORVET endosomal tethering complexes which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Functions predominantly in APPL1-containing endosomes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC032214" in other vectors (4)
SKU Description Size Price
MG210699 Vps8 (tGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$927.00
MR210699 Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$589.00 $727.00
MR210699L3 Lenti ORF clone of Vps8 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$1,027.00
MR210699L4 Lenti ORF clone of Vps8 (mGFP-tagged) - Mouse vacuolar protein sorting 8 homolog (S. cerevisiae) (cDNA clone MGC:38232 IMAGE:5323693) 10 ug
$1,027.00

file-alt Citations

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