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Dnajc10 (NM_024181) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210690
Dnajc10 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $727.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dnajc10
Synonyms 1200006L06Rik; D2Ertd706e; ERdj5; JPDI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210690 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGTCTGGTTAAACAAAGATGACTTTATCAGAGACTTGAAGAGGATCAGTCTCTGTCTTCTGATAC
TGTACGTGGTCGTTGTAGTGGGCACAGATCAGAATTTTTACAGTTTACTTGGAGTATCTAAAACTGCAAG
CAGTAGAGAAATAAGACAAGCCTTTAAGAAATTAGCACTGAAGTTACATCCTGATAAAAACCCGAATAAC
CCAAATGCACATGGTGATTTTTTAAAAATAAATAGAGCATATGAAGTACTCAAAGATGAAGATCTACGGA
AAAAGTATGACAAATATGGAGAAAAGGGACTTGAAGATAATCAAGGAGGCCAGTATGAGAGCTGGAGCTA
CTACCGTTACGATTTCGGTATTTACGATGATGATCCTGAAATCATAACATTAGAAAGAAGAGAATTTGAT
GCTGCTGTCAACTCTGGAGAACTGTGGTTTGTAAACTTTTACTCCCCAGGATGTTCGCATTGCCATGATT
TAGCCCCCACTTGGAGAGAATTTGCTAAAGAAGTGGATGGGTTGCTTCGAATCGGAGCTGTCAACTGTGG
TGATGATCGGATGCTCTGCCGAATGAAAGGAGTTAACAGCTACCCCAGCCTCTTCATCTTTAGGTCTGGA
ATGGCTGCAGTGAAGTACAATGGAGATCGGTCAAAGGAGAGCTTAGTGGCCTTCGCCATGCAGCACGTCA
GGAGCACAGTGACAGAGCTGTCAACAGGAAATTTTGTTAATGCCATAGAAACTGCTTTTGCTGCTGGTGT
TGGCTGGCTGATCACATTCTGCTCTAAAGGAGAAGATTGTTTGACGTCACAGACCCGACTCAGGCTTAGT
GGCATGTTGGATGGCCTTGTTAACGTGGGATGGGTGGACTGTGACGCCCAGGACAGCCTCTGCAAGAGCC
TGGACACCACAGCCAGCACCACGGCTTATTTCCCTCCTGGAGCCACTTTGAATGACAGGGGAAAATCCAG
TGTGTTGTTTCTTAATTCATTGGATGCTAAGGAAATATACATGGAAATCATACATAATCTTCCAGACTTT
GAACTGCTATCAGCAAATCAACTAGAGGATCGTTTGGCACATCATCGCTGGCTTGTATTTTTTCATTTTG
GAAAAAATGAAAACGCAAATGATCCTGAGTTGAAAAAACTGAAAACTCTACTTAAAAATGAGCATATTCA
AGTTGGAAGGTTTGATTGCTCTTCTGCACCGGGCATCTGCTCTGATCTTTATGTCTTCCAGCCCTGCCTA
GCAGTGTTTAAAGGACAAGGAACCAAAGAATATGAAACTCATCATGGAAAGAAGATTCTATACGATATAC
TTGCTTTTGCCAAAGAAAGTGTTAATTCTCATGTTACCACACTTGGACCTCAGAACTTCCCTGCAAGTGA
CAAGGAACCATGGCTTGTTGACTTCTTTGCCCCTTGGTGTCCGCCATGTCGTGCGCTTTTGCCGGAGTTA
CGGAAAGCATCAACACTGCTCTACGGTCAACTCAAAGTTGGTACCTTAGACTGTACAATTCATGAAGGAC
TCTGCAACATGTATAACATCCAAGCTTATCCAACGACAGTGGTATTCAATCAGTCCAGTATTCATGAGTA
TGAAGGACATCACTCTGCGGAACAGATCTTGGAGTTCATAGAGGATCTCAGAAACCCTTCAGTGGTCTCT
CTTACACCCAGCACTTTCAATGAGCTGGTTAAACAGAGAAAGCATGATGAAGTCTGGATGGTCGATTTCT
ATTCTCCATGGTGTCATCCCTGTCAGGTCTTGATGCCAGAATGGAAACGCATGGCCCGGACGTTAACTGG
ACTGATCAATGTAGGCAGTGTGGACTGCCAACAGTATCATTCTTTTTGTACTCAAGAAAATGTTCAAAGA
TACCCCGAGATAAGATTTTATCCCCAAAAGTCAAGTAAAGCCTATCAGTATCATAGTTACAATGGCTGGA
ATAGGGATGCGTATTCCCTGAGAAGCTGGGGTCTTGGATTTTTGCCCCAAGCATCCATAGATTTAACACC
TCAGACTTTCAATGAAAAAGTTCTACAAGGGAAAACTCACTGGGTGGTTGATTTCTATGCTCCTTGGTGT
GGACCTTGCCAGAATTTTGCTCCTGAGTTTGAACTCTTGGCTAGGATGATCAAAGGAAAAGTGAGAGCCG
GGAAAGTGGACTGTCAGGCTTACCCCCAGACGTGCCAGAAAGCCGGCATCAAAGCCTACCCCAGTGTCAA
ACTGTACCAGTACGAGAGAGCAAAGAAAAGTATTTGGGAAGAGCAAATAAATTCCAGAGATGCAAAAACT
ATTGCTGCCTTAATTTATGGAAAATTGGAAACGCTCCAAAGCCAAGTAAAAAGAAATAAGGATGAGCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210690 protein sequence
Red=Cloning site Green=Tags(s)

MGVWLNKDDFIRDLKRISLCLLILYVVVVVGTDQNFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNN
PNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWSYYRYDFGIYDDDPEIITLERREFD
AAVNSGELWFVNFYSPGCSHCHDLAPTWREFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSG
MAAVKYNGDRSKESLVAFAMQHVRSTVTELSTGNFVNAIETAFAAGVGWLITFCSKGEDCLTSQTRLRLS
GMLDGLVNVGWVDCDAQDSLCKSLDTTASTTAYFPPGATLNDRGKSSVLFLNSLDAKEIYMEIIHNLPDF
ELLSANQLEDRLAHHRWLVFFHFGKNENANDPELKKLKTLLKNEHIQVGRFDCSSAPGICSDLYVFQPCL
AVFKGQGTKEYETHHGKKILYDILAFAKESVNSHVTTLGPQNFPASDKEPWLVDFFAPWCPPCRALLPEL
RKASTLLYGQLKVGTLDCTIHEGLCNMYNIQAYPTTVVFNQSSIHEYEGHHSAEQILEFIEDLRNPSVVS
LTPSTFNELVKQRKHDEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSVDCQQYHSFCTQENVQR
YPEIRFYPQKSSKAYQYHSYNGWNRDAYSLRSWGLGFLPQASIDLTPQTFNEKVLQGKTHWVVDFYAPWC
GPCQNFAPEFELLARMIKGKVRAGKVDCQAYPQTCQKAGIKAYPSVKLYQYERAKKSIWEEQINSRDAKT
IAALIYGKLETLQSQVKRNKDEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024181
ORF Size 2379 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024181.2, NP_077143.2
RefSeq Size 4054 bp
RefSeq ORF 2382 bp
Locus ID 66861
UniProt ID Q9DC23
Cytogenetics 2 C3
MW 90.5 kDa
Summary Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.UniProtKB/Swiss-Prot Function
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Documents
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"NM_024181" in other vectors (3)
SKU Description Size Price
MC205861 Dnajc10 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10), (10ug) 10 ug
$727.00
MR210690L3 Lenti ORF clone of Dnajc10 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10) 10 ug
$1,027.00
MR210690L4 Lenti ORF clone of Dnajc10 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 10 (Dnajc10) 10 ug
$1,027.00

Plasmid Map for MR210690

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210690
Circular map for MR210690

file-alt Citations

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