OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Kifap3 (BC049100) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR210688
Kifap3 (Myc-DDK-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $726.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Kifap3 or BC049100"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kifap3
Synonyms KAP3, SMAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210688 representing BC049100
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAAGGCGAGGACGCCAGATACCTCAAAAGGAAAGTTAAAGGGGGGAATATTGATGTACATCCATCAG
AAAAAGCTCTCATTGTTCAGTATGAAGTGGAAGCTACCATTCTTGGAGAAATGGGAGATCCCATGTTGGG
AGAACGAAAGGAATGCCAAAAAATCATCCGTCTGAAGAGTCTCAATGCTAACACAGACATCACCTCTCTG
GCCCGGAAGGTGGTTGAGGAATGTAAGCTCATCCATCCCTCAAAGCTAAGCGAGGTGGAGCAGCTTTTGT
ACTATCTACAGAACCGCCGAGACTCATTGCCGGGAAAAGAAAAAAAAGAAAAATCAAGCAAGCCTAAAGA
CCCACCTCCTTTTGAAGGGATGGAGATTGACGAAGTGGCCAACATTAATGACATGGACGAATACATTGAG
CTCTTGTATGAAGACATTCCAGATAAGGTTCGGGGTTCTGCCTTGATCCTACAGCTTGCTCGAAATCCTG
ATAACCTGGAAGAGCTATTATTAAATGAAACTGCCCTGGGTGCGCTAGCAAGAGTCCTGAGAGAAGACTG
GAAACAAAGTGTTGAGTTAGCTACAAACATCATTTATATCTTCTTTTGCTTTTCTAGCTTTTCTCATTTT
CATGGACTCATCACTCACTACAAAATTGGAGCACTGTGTATGAATATCATTGATCATGAGTTAAAAAGAC
ACGAGCTTTGGCAAGAAGAACTCTCTAAGAAGAAGAAAGCTGTTGATGAAGACCTTGAAAATCAAACATT
GAGAAAGGATTATGACAAAACCTTTAAAAAATACCAAGGACTTGTGGTAAAACAAGAGCAGCTACTGAGA
GTTGCTCTCTACTTGCTTTTGAATCTTGCGGAGGACACACGTACAGAACTGAAGATGAGGAACAAGAATA
TCGTTCACATGTTGGTGAAGGCTCTTGATCGGGACAATTTTGAGCTGCTGATTCTGGTCGTGTCATTCTT
AAAGAAACTGAGTATTTTTATGGAGAATAAAAATGACATGGTAGAGATGGATATTGTTGAAAAACTGGTA
AAAATGATACCCTGTGAGCATGAAGATCTCCTGAATATCACCCTCCGGCTCCTCTTAAACCTCTCGTTTG
ACACAGGCCTGAGGAACAAGATGGTACAAGTTGGGCTTCTCCCAAAGCTCACTGCACTCCTGGGCAATGA
AAACTACAAACAAATAGCAATGTGTGTGCTTTACCATATAAGCATGGATGACCGCTTTAAGTCAATGTTT
GCATATACTGACTGCATACCACAGTTAATGAAGATGCTCTTTGAATGTTCAGATGAACGAATTGACTTGG
AGCTGATTTCTTTCTGCATTAATCTTGCTGCTAACAAGAGAAATGTCCAGCTCATCTGTGAAGGAAATGG
GCTGAAAATGCTCATGAAAAGAGCTCTGAAGCTCAAGGACCCACTGCTGATGAAGATGATCAGAAACATC
TCCCAGCATGATGGGCCCACTAAGAATTTGTTCATTGATTATGTTGGGGACCTTGCAGCCCAGATTTCCA
GTGATGAAGAGGAGGAGTTTGTAATCGAGTGTTTGGGAACGCTGGCAAATCTGACAATCCCAGATCTAGA
CTGGGAACTGGTCCTGAAGGAGTACAAGCTGGTTCCATTCCTCAAAGACAAACTAAAGCCAGGTGCCGCA
GAGGATGACCTTGTTTTAGAAGTGGTTATCATGATTGGGACGGTGTCTATGGATGACTCTTGTGCTGCCC
TACTGGCCAAATCTGGGATAATCCCAGCCCTCATCGAGCTGCTGAACGCTCAACAAGAAGATGATGAATT
TGTGTGTCAAATAATCTATGTCTTCTACCAGATGGTGTTCCATCAGGCCACAAGAGATGTCATAATCAAG
GAAACACAAGCTCCAGCATATCTCATTGACCTGATGCATGATAAAAATAATGAAATCCGGAAAGTCTGTG
ATAACACATTAGATATCATCGCAGAGTATGATGAAGAGTGGGCCAAGAAAATTCAGAGTGAGAAGTTTCG
CTGGCATAACTCTCAGTGGCTGGAGATGGTGGAGAGCCGTCAGCTGGATGAGAGCGAGCAGTACTTGTAT
GGTGATGATCGCATTGAGCCGTACATCCATGAAGGGGACATTCTTGAAAGGCCTGACCTTTTCTACAACT
CAGACGGACTAATTACCTCTGAAGGAGCCATAAGTCCAGACTTCTTCAATGATTTTCACCTCCAGAATGG
AGATGTGGTGGGGCAACACGCATTTCCTGGCAGCCTCGGGATGGATGGCTTCGGGCAGCCACTGGGGATC
CTTGGACGTCCTGCCACCGCGTATGGATTCCGCCCTGACGAACCTTACTACTACAGCTTTGGGTCTCGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210688 representing BC049100
Red=Cloning site Green=Tags(s)

MQGEDARYLKRKVKGGNIDVHPSEKALIVQYEVEATILGEMGDPMLGERKECQKIIRLKSLNANTDITSL
ARKVVEECKLIHPSKLSEVEQLLYYLQNRRDSLPGKEKKEKSSKPKDPPPFEGMEIDEVANINDMDEYIE
LLYEDIPDKVRGSALILQLARNPDNLEELLLNETALGALARVLREDWKQSVELATNIIYIFFCFSSFSHF
HGLITHYKIGALCMNIIDHELKRHELWQEELSKKKKAVDEDLENQTLRKDYDKTFKKYQGLVVKQEQLLR
VALYLLLNLAEDTRTELKMRNKNIVHMLVKALDRDNFELLILVVSFLKKLSIFMENKNDMVEMDIVEKLV
KMIPCEHEDLLNITLRLLLNLSFDTGLRNKMVQVGLLPKLTALLGNENYKQIAMCVLYHISMDDRFKSMF
AYTDCIPQLMKMLFECSDERIDLELISFCINLAANKRNVQLICEGNGLKMLMKRALKLKDPLLMKMIRNI
SQHDGPTKNLFIDYVGDLAAQISSDEEEEFVIECLGTLANLTIPDLDWELVLKEYKLVPFLKDKLKPGAA
EDDLVLEVVIMIGTVSMDDSCAALLAKSGIIPALIELLNAQQEDDEFVCQIIYVFYQMVFHQATRDVIIK
ETQAPAYLIDLMHDKNNEIRKVCDNTLDIIAEYDEEWAKKIQSEKFRWHNSQWLEMVESRQLDESEQYLY
GDDRIEPYIHEGDILERPDLFYNSDGLITSEGAISPDFFNDFHLQNGDVVGQHAFPGSLGMDGFGQPLGI
LGRPATAYGFRPDEPYYYSFGSR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC049100
ORF Size 2379 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC049100.1
RefSeq Size 3952 bp
RefSeq ORF 2381 bp
Locus ID 16579
Cytogenetics 1 H2.1
MW 144.9 kDa
Summary The protein encoded by this gene is the non-motor subunit of kinesin-2 complex, and forms a heterotrimer with two members of the kinesin superfamily of proteins that together form a microtubule plus-end directed translocator that plays an important role in intracellular transport, mitosis, and cell-cell adhesion. This protein contains multiple armadillo repeats involved in protein binding, and may serve as an adaptor to regulate binding of cargo with the motor proteins. Conditional disruption of this gene in mouse neural precursor cells caused a tumor-like phenotype and defective organization of the neuroepithelium thought to be the result of altered N-cadherin subcellular localization. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Mar 2015
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"BC049100" in other vectors (4)
SKU Description Size Price
MC220731 Kifap3 (untagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182), (10ug) 10 ug
$800.00
MG210688 Kifap3 (tGFP-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) 10 ug
$926.00
MR210688L3 Lenti ORF clone of Kifap3 (Myc-DDK-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) 10 ug
$1,026.00
MR210688L4 Lenti ORF clone of Kifap3 (mGFP-tagged) - Mouse kinesin-associated protein 3 (cDNA clone MGC:61298 IMAGE:5698182) 10 ug
$1,026.00

Plasmid Map for MR210688

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210688
Circular map for MR210688

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products