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Atxn1 (NM_009124) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210675
Atxn1 (Myc-DDK-tagged) - Mouse ataxin 1 (Atxn1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $725.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Atxn1
Synonyms 2900016G23Rik; Atx1; C85907; ENSMUSG00000074917; Gm10786; Sca1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210675 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATCCAACCAAGAGCGGAGCAACGAATGCCTGCCTCCCAAGAAACGTGAGATCCCCGCCACCAGCC
GGCCCTCGGAGGAGAAGGCCACTGCTCTGCCCAGCGACAACCACTGCGTGGAGGGTGTGGCCTGGCTCCC
CAGCACCCCTGGCATCCGCGGCCATGGGGGTGGGCGGCACGGGTCAGCAGGGACTTCCGGGGAGCATGGT
TTACAAGGAATGGGTTTACATAAAGCACTGTCCGCAGGGCTGGATTACTCCCCACCCAGTGCCCCCAGGT
CAGTCCCCACAGCCAACACGCTGCCCACCGTGTACCCTCCTCCTCAGTCAGGGACCCCGGTGTCTCCTGT
GCAGTACGCCCACCTTTCGCATACCTTCCAGTTCATTGGGTCCTCCCAATACAGTGGGCCTTACGCGGGC
TTTATCCCTTCCCAGCTGATCTCCCCATCAGGCAACCCGGTCACCAGTGCAGTAGCCTCAGCTGCAGGGG
CCACCACTCCATCACAGCGCTCCCAGCTGGAGGCTTATTCCACCCTGCTGGCCAACATGGGCAGTCTGAG
CCAGGCACCAGGACATAAGGTTGAGCCCCCTCCGCAGCAGCACCTCAGCAGGGCTGCAGGATTAGTCAAC
CCGGGGTCCCCTCCTCCACCCACCCAGCAGAACCAGTACATCCATATTTCCAGCTCTCCACAGAGCTCCG
GGCGGGCGACATCTCCCCCACCCATCCCGGTCCACCTCCATCCCCATCAGACGATGATCCCGCACACACT
CACCCTGGGGCCTTCATCCCAGGTGGTTGTGCAATATAGTGATGCCGGAGGCCACTTTGTTCCTCGAGAG
TCCACCAAAAAAGCCGAGAGCAGCAGGTTGCAGCAGGCTATGCAAGCCAAGGAAGTCCTGAATGGGGAGA
TGGAGAAAAGCCGGAGGTATGGGGCATCATCTTCTGTGGAGCTGAGCCTAGGCAAGGCAAGCAGTAAGTC
AGTGCCTCATCCCTATGAGTCCAGGCATGTGGTGGTCCACCCAAGCCCAGCAGACTACAGCAGTCGTGAT
ACCTCCGGGGTCCGTGGATCTGTGATGGTTCTGCCTAATAGCAGCACACCCTCAGCCGACCTGGAGGCCC
AGCAGACCACGCATCGAGAGGCCTCCCCATCCACCCTCAATGACAAGAGCGGCCTGCACCTAGGGAAGCC
GGGCCACAGGTCTTATGCGCTGTCCCCCCACACGGTCATTCAGACCACACACAGTGCATCAGAGCCTCTC
CCGGTGGGCCTACCAGCCACGGCCTTCTACGCTGGCACTCAACCTCCTGTCATCGGCTACCTGAGCGGCC
AGCAGCAAGCAATCACCTATGCTGGTGGTCTGCCGCAGCACCTGGTGATCCCAGGTAACCAGCCCCTGCT
CATCCCGGTGGGCAGCCCTGACATGGACACGCCTGGGGCAGCCTCGGCCATCGTGACGTCATCACCCCAG
TTTGCTGCAGTACCTCACACGTTTGTCACCACCGCCCTGCCCAAGAGCGAGAACTTCAACCCAGAGGCTC
TGGTCACCCAGGCTGCCTACCCAGCCATGGTGCAGGCCCAGATCCACCTGCCGGTGGTGCAGTCCGTGGC
GTCCCCCACCACGGCGTCTCCCACGCTGCCGCCATATTTCATGAAAGGCTCCATCATCCAGCTGGCCAAC
GGGGAGCTGAAGAAGGTGGAGGACCTGAAGACGGAGGATTTCATCCAGAGTGCAGAGATTAGCAATGACC
TCAAGATCGACTCCAGTACTGTGGAGAGAATCGAGGAGAGCCACAGCCCCGGGGTGGCCGTGATACAGTT
TGCTGTTGGTGAACACCGAGCCCAGGTCAGTGTCGAAGTCTTGGTAGAGTATCCTTTTTTTGTATTTGGA
CAGGGCTGGTCATCCTGCTGTCCTGAGCGGACCAGCCAGCTCTTTGATCTGCCGTGTTCCAAACTCTCTG
TTGGGGACGTCTGCATCTCGCTCACCCTCAAGAACCTGAAGAATGGCTCTGTTAAAAAGGGCCAGCCTGT
GGACCCTGCCAGCGTCCTGCTGAAGCAGGCAAAGACCGACAGCCTGGCTGGCAGCAGACACAGATACGCG
GAGCAGGAAAACGGAATCAACCAGGGAAGCGCCCAGGTGCTCTCTGAGAATGGCGAACTGAAGTTTCCAG
AAAAAATAGGATTGCCTGCAGCACCCTTCCTCAGCAAAATAGAACCGAGCAAACCCACAGCCACGAGGAA
GAGGAGGTGGTCGGCGCCGGAGACCCGTAAACTGGAGAAGTCGGAGGACGAGCCACCTTTGACTCTTCCC
AAGCCTTCGCTCATTCCTCAGGAGGTTAAGATCTGCATCGAAGGCCGATCTAACGTGGGCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210675 protein sequence
Red=Cloning site Green=Tags(s)

MKSNQERSNECLPPKKREIPATSRPSEEKATALPSDNHCVEGVAWLPSTPGIRGHGGGRHGSAGTSGEHG
LQGMGLHKALSAGLDYSPPSAPRSVPTANTLPTVYPPPQSGTPVSPVQYAHLSHTFQFIGSSQYSGPYAG
FIPSQLISPSGNPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQAPGHKVEPPPQQHLSRAAGLVN
PGSPPPPTQQNQYIHISSSPQSSGRATSPPPIPVHLHPHQTMIPHTLTLGPSSQVVVQYSDAGGHFVPRE
STKKAESSRLQQAMQAKEVLNGEMEKSRRYGASSSVELSLGKASSKSVPHPYESRHVVVHPSPADYSSRD
TSGVRGSVMVLPNSSTPSADLEAQQTTHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPL
PVGLPATAFYAGTQPPVIGYLSGQQQAITYAGGLPQHLVIPGNQPLLIPVGSPDMDTPGAASAIVTSSPQ
FAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPTTASPTLPPYFMKGSIIQLAN
GELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEESHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFG
QGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKQAKTDSLAGSRHRYA
EQENGINQGSAQVLSENGELKFPEKIGLPAAPFLSKIEPSKPTATRKRRWSAPETRKLEKSEDEPPLTLP
KPSLIPQEVKICIEGRSNVGK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009124
ORF Size 2373 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009124.6
RefSeq Size 10599 bp
RefSeq ORF 2376 bp
Locus ID 20238
UniProt ID P54254
Cytogenetics 13 21.98 cM
MW 83.8 kDa
Summary Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed:28288114).UniProtKB/Swiss-Prot Function
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"NM_009124" in other vectors (4)
SKU Description Size Price
MC202345 Atxn1 (untagged) - Mouse ataxin 1 (Atxn1), (10ug) 10 ug
$725.00
MG210675 Atxn1 (tGFP-tagged) - Mouse ataxin 1 (Atxn1) 10 ug
$925.00
MR210675L3 Lenti ORF clone of Atxn1 (Myc-DDK-tagged) - Mouse ataxin 1 (Atxn1) 10 ug
$1,025.00
MR210675L4 Lenti ORF clone of Atxn1 (mGFP-tagged) - Mouse ataxin 1 (Atxn1) 10 ug
$1,025.00

Plasmid Map for MR210675

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210675
Circular map for MR210675

file-alt Citations

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