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Ripk4 (NM_023663) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210650
Ripk4 (Myc-DDK-tagged) - Mouse receptor-interacting serine-threonine kinase 4 (Ripk4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $721.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ripk4
Synonyms 2310069J12Rik; AI552420; ANKK2; Ankrd3; DIk; PKK; RIP4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210650 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCGAGGGCCGGGGCCGGTGGGCTCTGGGGCTGCTGCGCACCTTCGACGCCGGCGAATTCGCAG
GCTGGGAGAAGGTGGGCTCGGGCGGCTTCGGGCAGGTGTACAAGGTGCGCCATGTGCACTGGAAGACGTG
GCTCGCGATCAAGTGCTCGCCCAGTCTGCACGTCGACGACAGGGAACGAATGGAGCTCCTGGAGGAAGCT
AAGAAGATGGAGATGGCCAAGTTCCGATACATTCTACCTGTGTACGGCATATGCCAGGAACCTGTCGGCT
TGGTCATGGAGTACATGGAGACAGGCTCCCTGGAGAAGCTGCTGGCCTCAGAGCCATTGCCTTGGGACCT
GCGCTTTCGCATCGTGCACGAGACAGCCGTGGGCATGAACTTCCTGCATTGCATGTCTCCGCCACTGCTG
CACCTAGACCTGAAGCCAGCGAACATCCTGCTGGATGCCCACTACCATGTCAAGATTTCTGACTTTGGGC
TGGCCAAGTGCAATGGCATGTCCCACTCTCATGACCTCAGCATGGATGGCCTGTTTGGTACAATCGCTTA
CCTCCCTCCAGAGCGAATTCGTGAGAAGAGCCGCTTGTTTGACACCAAACATGATGTATACAGCTTCGCC
ATTGTGATCTGGGGTGTGCTTACACAGAAGAAGCCATTTGCAGATGAAAAGAACATCCTACACATCATGA
TGAAAGTGGTAAAGGGCCACCGCCCAGAGCTGCCACCCATCTGCAGACCCCGGCCGCGTGCCTGTGCCAG
CCTGATAGGGCTCATGCAACGGTGCTGGCATGCAGACCCACAGGTGCGGCCCACCTTCCAAGAAATTACC
TCTGAAACAGAAGACCTTTGTGAGAAGCCTGATGAGGAGGTGAAAGACCTGGCTCATGAGCCAGGCGAGA
AAAGCTCTCTAGAGTCCAAGAGTGAGGCCAGGCCCGAGTCCTCACGCCTCAAGCGCGCCTCTGCTCCCCC
CTTCGATAACGACTGCAGTCTCTCCGAGTTGCTGTCACAGTTGGACTCTGGGATCTCCCAGACTCTTGAA
GGCCCCGAAGAGCTCAGCCGAAGTTCCTCTGAATGCAAGCTCCCATCGTCCAGCAGTGGCAAGAGGCTCT
CGGGGGTGTCCTCAGTGGACTCAGCCTTTTCCTCCAGAGGATCGCTGTCACTGTCTTTTGAGCGGGAAGC
TTCAACAGGCGACCTGGGCCCCACAGACATCCAGAAGAAGAAGCTAGTGGATGCCATCATATCAGGGGAC
ACCAGCAGGCTGATGAAGATCCTACAGCCCCAAGATGTGGACTTGGTTCTAGACAGCAGTGCCAGCCTGC
TGCACCTGGCTGTGGAGGCCGGACAGGAGGAGTGTGTCAAGTGGCTGCTGCTTAACAATGCCAACCCCAA
CCTGACCAACAGGAAGGGCTCTACACCACTGCATATGGCTGTGGAGCGGAAGGGACGTGGAATTGTGGAG
CTACTGCTAGCCCGGAAGACCAGTGTCAATGCCAAGGATGAAGACCAGTGGACTGCCCTGCACTTTGCAG
CCCAGAATGGGGATGAGGCCAGCACAAGGCTGCTGCTAGAGAAGAATGCTTCTGTCAATGAGGTGGACTT
TGAGGGCCGAACACCCATGCATGTAGCCTGCCAGCATGGACAGGAGAACATTGTGCGCACCCTGCTCCGC
CGTGGTGTGGATGTGGGCCTGCAGGGAAAGGATGCCTGGTTGCCTCTGCACTATGCTGCCTGGCAGGGCC
ACCTTCCCATTGTTAAGCTGCTAGCCAAGCAGCCTGGGGTGAGTGTGAATGCCCAGACACTAGACGGGAG
GACACCCCTGCACCTGGCTGCTCAGAGGGGGCATTACCGTGTGGCTCGCATTCTCATTGACCTGTGCTCT
GATGTTAACATCTGCAGCCTACAGGCACAGACACCTCTGCATGTTGCTGCAGAGACTGGACACACTAGTA
CTGCCAGGCTACTCTTGCATCGTGGTGCTGGCAAGGAGGCTTTGACCTCAGAGGGCTATACTGCCTTGCA
CCTGGCAGCCCAGAATGGACACCTGGCTACTGTCAAGCTGCTCATAGAGGAGAAGGCTGATGTGATGGCT
CGGGGTCCCCTGAATCAGACAGCACTGCACCTGGCTGCTGCCCGTGGACACTCAGAGGTGGTAGAAGAGC
TGGTCAGTGCTGACCTCATTGACCTGTCTGATGAGCAGGGCCTCAGCGCACTGCACCTGGCTGCTCAGGG
CAGGCATTCACAGACTGTGGAGACACTGCTCAAACATGGAGCACACATCAACTTGCAGAGTCTCAAGTTC
CAAGGAGGCCAGAGCTCTGCTGCCACGTTGCTCCGACGCAGCAAGACC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210650 protein sequence
Red=Cloning site Green=Tags(s)

MEGEGRGRWALGLLRTFDAGEFAGWEKVGSGGFGQVYKVRHVHWKTWLAIKCSPSLHVDDRERMELLEEA
KKMEMAKFRYILPVYGICQEPVGLVMEYMETGSLEKLLASEPLPWDLRFRIVHETAVGMNFLHCMSPPLL
HLDLKPANILLDAHYHVKISDFGLAKCNGMSHSHDLSMDGLFGTIAYLPPERIREKSRLFDTKHDVYSFA
IVIWGVLTQKKPFADEKNILHIMMKVVKGHRPELPPICRPRPRACASLIGLMQRCWHADPQVRPTFQEIT
SETEDLCEKPDEEVKDLAHEPGEKSSLESKSEARPESSRLKRASAPPFDNDCSLSELLSQLDSGISQTLE
GPEELSRSSSECKLPSSSSGKRLSGVSSVDSAFSSRGSLSLSFEREASTGDLGPTDIQKKKLVDAIISGD
TSRLMKILQPQDVDLVLDSSASLLHLAVEAGQEECVKWLLLNNANPNLTNRKGSTPLHMAVERKGRGIVE
LLLARKTSVNAKDEDQWTALHFAAQNGDEASTRLLLEKNASVNEVDFEGRTPMHVACQHGQENIVRTLLR
RGVDVGLQGKDAWLPLHYAAWQGHLPIVKLLAKQPGVSVNAQTLDGRTPLHLAAQRGHYRVARILIDLCS
DVNICSLQAQTPLHVAAETGHTSTARLLLHRGAGKEALTSEGYTALHLAAQNGHLATVKLLIEEKADVMA
RGPLNQTALHLAAARGHSEVVEELVSADLIDLSDEQGLSALHLAAQGRHSQTVETLLKHGAHINLQSLKF
QGGQSSAATLLRRSKT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023663
ORF Size 2358 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023663.7
RefSeq Size 3550 bp
RefSeq ORF 2361 bp
Locus ID 72388
UniProt ID Q9ERK0
Cytogenetics 16 C4
MW 86.6 kDa
Summary Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023663" in other vectors (4)
SKU Description Size Price
MC221549 Ripk4 (untagged) - Mouse receptor-interacting serine-threonine kinase 4 (Ripk4), (10ug) 10 ug
$793.00
MG210650 Ripk4 (tGFP-tagged) - Mouse receptor-interacting serine-threonine kinase 4 (Ripk4) 10 ug
$921.00
MR210650L3 Lenti ORF clone of Ripk4 (Myc-DDK-tagged) - Mouse receptor-interacting serine-threonine kinase 4 (Ripk4) 10 ug
$1,021.00
MR210650L4 Lenti ORF clone of Ripk4 (mGFP-tagged) - Mouse receptor-interacting serine-threonine kinase 4 (Ripk4) 10 ug
$1,021.00

Plasmid Map for MR210650

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210650
Circular map for MR210650

file-alt Citations

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