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Cog4 (NM_133973) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210645
Cog4 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 4 (Cog4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $720.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cog4
Synonyms AW554810; D8Ertd515e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210645 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGTGGAGTCTCCGCTGAAATTGTCAGGAGCTCCTCCGCCACCGGAGGGGGTGGGAGGAGGAC
ACTGCTCCGAAATCTCCACCGAGCTCATTCGCTCTCTGACAGAGCTGCAGGAGCTGGAAGCTGTGTACGA
ACGACTCTGTGGCGAGGAGAAAGCGGTGGAGAAAGAACTGGATGCTCTTTTGGAACAGCAGAACACCATT
GAAAGTAAGATGGTCACTCTCCACAGGATGGGTCCCAGCCTGCAGTTGATTGAGGGAGACGCAAAGCAAC
TGGCTGGAATGATCACCTTTACCTGCAGCCTAGCTGAAAACGTGTCGAGCAAAGTCCGTCAGCTTGACCT
GGCCAAGAACCGCCTCTATCAGGCCATTCAGAGAGCCGATGACATCTTGGACCTGAAGTTCTGCATGGAT
GGAGTTCAGACTGCTTTGAGGAATGAGGATTATGAGCAGGCTGCAGCCCACATTCATCGTTACTTGTGCC
TGGACAAGTCAGTCATTGAGCTCAGCCGACAAGGCAAAGAAGGAAGCATGATTGATGCCAACCTGAAATT
ACTGCAGGAAGCTGAGCAACGTCTTAAGGCCATAGTCGCAGAGAAGTTTGCTATCGCTACCAAAGAAGGT
GATTTGCCCCAGGTGGAGCGATTCTTCAAGATCTTCCCACTGCTGGGTTTACATGAGGATGGCCTGAGCA
AGTTCTCGGAATACCTGTGCAAACAGGTCGCCAGTAAGGCTGAGGAGAATCTGTTGTTGGTGCTGGGCTC
AGACATGAGTGATCGGAGAGCTGCGGTCATCTTTGCGGATACTCTCACACTCCTGTTTGAAGGGATCGCC
CGAATTGTGGAAACTCACCAACCCATCGTGGAGACTTACTATGGACCAGGGCGACTCTTTACCCTGATCA
AATATCTTCAAGTAGAATGTGACACACAGGTGGAGAAGGTCGTAAACAAGTTCATCAAGCAAAGGGACTA
CCACCAGCAGTTTCGGCTCGTCCAGAGTAATTTGATGAGAAATTCTGCAACAGAAAAAATTGAACCAAGG
GAGCTGGACCCTGTCTTGACTGAGGTTACCCTGATGAATGCGCGAAGTGAACTGTACTTACGCTTCCTCA
GGAAGAGGATCAGCGCGGATTTTGAGGTGGGGGACTCCATGGCTTCAGAAGAAGTAAAGCAAGAGCACCA
GAAGTGCCTGGACAAACTCCTCAATAACTGCCTATTGAGCTGTACCATGCAGGAGCTAATTGGCTTCTAT
ATTACCATGGAGGAGTACTTCATGAGAGAGACGGTCAATAAGGCTGTGGCTCTGGATACCTATGAGAAAG
GCCAGTTGACATCCAGCATGGTGGATGATGTCTTCTACATTGTCAAAAAGTGCATTGGGCGGGCTCTGTC
CAGCTCTAACATCGACTGTCTCTGTGCCATGATCAACCTTGCCACCAGAGAGCTGGAAGCTGACTTCAGG
GATGTTCTATGTAATAAGCTAAGAATGGGCTTCCCAGCCACCACCTTACAGGACATCCAGCGTGGGGTGA
CAAGTGCCGTGAACATCATGCACAGCAGCCTCCAGCAGGGCAAGTTTGACACCAAAGGCATCGAGAGCAC
TGATGAAGCCAAGCTGTCCTTCCTGGTGACTCTGAACAATGTGGAAGTCTGCAGTGAAAACATCTCAACT
CTGAAGAAGACTCTGGAGAGTGACTGCACCAAGCTCTTCAGTCAGGGCATTGGAGGGGAACAGGCCCAGG
CCAAGTTTGACAGCTGCCTTTCTGACTTAGCTGCTGTGTCCAACAAATTTCGAGACCTCTTGCAGGAAGG
GTTGGCGGAGCTCAACAGTTCAGCAGTCAAGCCGCAGGTGCAACCTTGGATCAACACTTTTCTCTCAGTC
TCGCACAGCATCGAGGAGGAAGAATTCAACGACTATGAGGCCAATGACCCCTGGGTACAGCAATTCATCC
TCAACCTGGAACAGCAGATGGCAGAGTTCAAGGCCAGCCTGTCCCCAGTCATCTATGACAGCCTAACCGG
CCTCATGACCAGCCTTGTTGCTGTTGAACTGGAAAAAGTGGTCCTGAAATCCACCTTCAACAGGCTGGGC
GGTCTGCAGTTTGACAAGGAACTCCGGTCACTCATTGCTTACCTTACCACTGTGACAACCTGGACCATCC
GAGACAAGTTCGCCCGCCTCTCTCAGATGGCTACAATCCTTAACCTAGAGCGGGTGACTGAGATACTAGA
TTACTGGGGTGCTAACTCTGGCCCTTTGACATGGCGCCTCACCCCTGCTGAAGTGCGACAGGTGCTGGCT
CTGCGCATAGACTTCCGCAATGAGGATATCAAGAGGCTGCGCCTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210645 protein sequence
Red=Cloning site Green=Tags(s)

MAEVESPLKLSGAPPPPEGVGGGHCSEISTELIRSLTELQELEAVYERLCGEEKAVEKELDALLEQQNTI
ESKMVTLHRMGPSLQLIEGDAKQLAGMITFTCSLAENVSSKVRQLDLAKNRLYQAIQRADDILDLKFCMD
GVQTALRNEDYEQAAAHIHRYLCLDKSVIELSRQGKEGSMIDANLKLLQEAEQRLKAIVAEKFAIATKEG
DLPQVERFFKIFPLLGLHEDGLSKFSEYLCKQVASKAEENLLLVLGSDMSDRRAAVIFADTLTLLFEGIA
RIVETHQPIVETYYGPGRLFTLIKYLQVECDTQVEKVVNKFIKQRDYHQQFRLVQSNLMRNSATEKIEPR
ELDPVLTEVTLMNARSELYLRFLRKRISADFEVGDSMASEEVKQEHQKCLDKLLNNCLLSCTMQELIGFY
ITMEEYFMRETVNKAVALDTYEKGQLTSSMVDDVFYIVKKCIGRALSSSNIDCLCAMINLATRELEADFR
DVLCNKLRMGFPATTLQDIQRGVTSAVNIMHSSLQQGKFDTKGIESTDEAKLSFLVTLNNVEVCSENIST
LKKTLESDCTKLFSQGIGGEQAQAKFDSCLSDLAAVSNKFRDLLQEGLAELNSSAVKPQVQPWINTFLSV
SHSIEEEEFNDYEANDPWVQQFILNLEQQMAEFKASLSPVIYDSLTGLMTSLVAVELEKVVLKSTFNRLG
GLQFDKELRSLIAYLTTVTTWTIRDKFARLSQMATILNLERVTEILDYWGANSGPLTWRLTPAEVRQVLA
LRIDFRNEDIKRLRL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133973
ORF Size 2355 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133973.2, NP_598734.1
RefSeq Size 2754 bp
RefSeq ORF 2358 bp
Locus ID 102339
UniProt ID Q8R1U1
Cytogenetics 8 57.76 cM
MW 88.7 kDa
Summary Required for normal Golgi function. Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with SCFD1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_133973" in other vectors (4)
SKU Description Size Price
MC204016 Cog4 (untagged) - Mouse component of oligomeric golgi complex 4 (Cog4), (10ug) 10 ug
$720.00
MG210645 Cog4 (tGFP-tagged) - Mouse component of oligomeric golgi complex 4 (Cog4) 10 ug
$920.00
MR210645L3 Lenti ORF clone of Cog4 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 4 (Cog4) 10 ug
$1,020.00
MR210645L4 Lenti ORF clone of Cog4 (mGFP-tagged) - Mouse component of oligomeric golgi complex 4 (Cog4) 10 ug
$1,020.00

Plasmid Map for MR210645

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210645
Circular map for MR210645

file-alt Citations

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