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Plcd3 (NM_152813) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210644
Plcd3 (Myc-DDK-tagged) - Mouse phospholipase C, delta 3 (Plcd3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $720.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Plcd3
Synonyms 2610205J15Rik; mKIAA1964
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210644 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGCGGTGGCTGGAAAAGGAGTCGCCGCTCGCCGGAGGAATCCCGGGTGTCCGCCCAGGTCGCAG
CGCCCCTCGCGTTCCCGCCCTCGCCCGCGTCGTCGGACAGCAGCACCAAAAGGCCTGGGCTACGGGCACT
GAAGAAGATGGGCCTGACGGAGGATGAGGATGTCCAAGCCATGCTGCGGGGCTCCCGACTCCTCAAGATC
CGCTCACGCACGTGGCACAAGGAGCGGCTGTACCGGCTGCAGGAGGACGGCCTGAGCGTGTGGTTTCAGC
GGCGCATCCCTCGCGCAGCTTCTAAGCACATATTCTTCGTGCAGCACATCGAGGCTGTCCGGGAGGGTCA
TCAGTCGGAGGGCCTGCGGCGCTTCGGGGGCGCCTTCGCGCCAGCGTGCTGCCTCACCATCGCCTTCAAG
GGCCGCCGCAAGAACCTGGACCTGGCAGCGCCCACTGCGGAGGAGGCACAGCGCTGGGTGCGAGGACTGG
CCAAGCTGCGGGCGCGCCTGGATGCCATGAGCCAGCGGGAGCGCCTAGACCATTGGATCCATTCCTATCT
GCACCGGGCCGACTCCGACCAAGACAGTAAGATGAGCTTCAAGGAGATCAAGAGCCTGCTGAGGATGGTG
AACGTGGACATGAACGACATGTACGCTTACCGCCTCTTCAAGGAGTGTGACCATTCCAACAACGAGCGTC
TAGAGGGGGCCGAGATAGAGGCGTTCCTGCGGAGGCTGCTAAAGCGGCCTGAGCTGGAGGAGATCTTCCG
CCGGTACTCCGGTGAGGACCGGGTGCTGAGTGCCTCTGAGCTGCTGGAGTTCCTGGAAGACCAGGGCGAG
GATGGTGCCACCCTGGCCTGTGCCCAGCAGCTTATCCAGACTTATGAGCTCAATGAGACAGCCAAGCAGC
ACGAACTGATGACGCTGGATGGCTTCATGATGTATCTGTTGTCGCCTGAGGGGGCCGCCCTGAACGTGGC
CCACACGTGTGTGTTCCAGGACATGGGTCAGCCCCTCGCCCACTACTTTATCTCGTCCTCTCACAATACC
TACCTGACGGACTCTCAGATCGGAGGGACCAGCAGCACAGAGGCTTATATTAGGGCCTTTGCCCAGGGAT
GCCGCTGTGTGGAACTTGACTGCTGGGAGGGGCCGGGAGGGGAACCTGTCATCTACCATGGCCACACGCT
CACCTCCAAGATCCTCTTTCGAGATGTGATCCAGGCTGTGCGTGACCACGCTTTCACGTCGTCCCCATAT
CCTGTAATCCTATCCCTCGAGAACCACTGCGGGCTGGAACAGCAGGCTGTCATGGCTCGCCACCTTCGAA
GCATCCTGGGAGACATGCTGGTGACACAGGCCCTGGACTCCCAGAACCCCGAGGAGCTGCCATCTCCTGA
GCAGCTCAAGGGCCGGATCTTGGTGAAGGGGAAGAAGCTGCCAGCTGCTCGGAGTGAGGATGGTCGAATT
CTGTCAGACAGGGAGGAGGAAGAGGAGGAGGAAGAGGAGGCGGAAGAGGCCTTGGAAGCTGCAGAGCAGA
GGAGTCGAGCCAAGCAGATCTCCCCAGAGCTCTCAGCGCTGGCTGTGTATTGCTGTGCTACCCGCCTGCG
GACCCTGGAACCCAGTCCTGGCCCGCCTCAGTCTTGCACGGTTGGCTCCCTCAGCGAGCGCAAGGCCAGG
AAGTTCACCAGGGAGGCAGGGACCAGCTTTGTCAGGCACAACACTCAGCAACTGACCCGAGTGTACCCAC
TGGGGCTGCGGATGAACTCAGCCAACTATAACCCCCAGGAGATGTGGAACTCCGGCTGTCAGCTGGTGGC
TCTGAACTTTCAGACTCCTGGTTATGAGATGGACCTCAACACAGGGCGCTTCCTCATAAACGGGCAATGT
GGCTACGTCCTCAAACCTGCTTACCTCAGGCAACTCAACACCACCTTTGACCCTGAGTGCCCTGGACCCC
CCAGAACTACTCTGGCAATCCAGGTGTTAACGGCCCAGCAGCTACCCAAGCTGAACGCAGAGAAGCCCAG
CTCCATTGTGGATCCTCTGGTGCGCGTGGAGATCCACGGGGTGCCCGAAGACTGTGCCCAGAAGGAGACA
GACTACGTCCTCAACAATGGCTTCAACCCCTGCTGGGAGCAGACTCTGCAGTTCCGACTGCGGGCCCCAG
AGCTGGTGCTGGTCAGGTTTGTGGTAGAAGATTATGACACCACCTCTCCCAATGATTTTGTGGGCCAGTC
TACACTGCCTCTCAGCAGCCTGAAGCAAGGGTACCGCCACATCCACCTGCTGTCCAAGGATGGAGCCTCG
CTCGCTCCAGCCACGCTCTTCGTGCACATCCGCATTCAGAACTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210644 protein sequence
Red=Cloning site Green=Tags(s)

MLCGGWKRSRRSPEESRVSAQVAAPLAFPPSPASSDSSTKRPGLRALKKMGLTEDEDVQAMLRGSRLLKI
RSRTWHKERLYRLQEDGLSVWFQRRIPRAASKHIFFVQHIEAVREGHQSEGLRRFGGAFAPACCLTIAFK
GRRKNLDLAAPTAEEAQRWVRGLAKLRARLDAMSQRERLDHWIHSYLHRADSDQDSKMSFKEIKSLLRMV
NVDMNDMYAYRLFKECDHSNNERLEGAEIEAFLRRLLKRPELEEIFRRYSGEDRVLSASELLEFLEDQGE
DGATLACAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALNVAHTCVFQDMGQPLAHYFISSSHNT
YLTDSQIGGTSSTEAYIRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVIQAVRDHAFTSSPY
PVILSLENHCGLEQQAVMARHLRSILGDMLVTQALDSQNPEELPSPEQLKGRILVKGKKLPAARSEDGRI
LSDREEEEEEEEEAEEALEAAEQRSRAKQISPELSALAVYCCATRLRTLEPSPGPPQSCTVGSLSERKAR
KFTREAGTSFVRHNTQQLTRVYPLGLRMNSANYNPQEMWNSGCQLVALNFQTPGYEMDLNTGRFLINGQC
GYVLKPAYLRQLNTTFDPECPGPPRTTLAIQVLTAQQLPKLNAEKPSSIVDPLVRVEIHGVPEDCAQKET
DYVLNNGFNPCWEQTLQFRLRAPELVLVRFVVEDYDTTSPNDFVGQSTLPLSSLKQGYRHIHLLSKDGAS
LAPATLFVHIRIQNS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_152813
ORF Size 2355 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_152813.2, NP_690026.1
RefSeq Size 3031 bp
RefSeq ORF 2358 bp
Locus ID 72469
UniProt ID Q8K2J0
Cytogenetics 11 66.71 cM
MW 88.6 kDa
Summary Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow.UniProtKB/Swiss-Prot Function
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"NM_152813" in other vectors (3)
SKU Description Size Price
MG210644 Plcd3 (tGFP-tagged) - Mouse phospholipase C, delta 3 (Plcd3) 10 ug
$789.00 $920.00
MR210644L3 Lenti ORF clone of Plcd3 (Myc-DDK-tagged) - Mouse phospholipase C, delta 3 (Plcd3) 10 ug
$1,020.00
MR210644L4 Lenti ORF clone of Plcd3 (mGFP-tagged) - Mouse phospholipase C, delta 3 (Plcd3) 10 ug
$1,020.00

Plasmid Map for MR210644

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210644
Circular map for MR210644

file-alt Citations

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