Pnpt1 (BC055826) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR210634
Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934)
$589.00
$717.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pnpt1 |
| Synonyms | Old35, PNPase, RP23-313I4.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC055826 |
| ORF Size | 2349 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC055826, AAH55826 |
| RefSeq Size | 2598 bp |
| RefSeq ORF | 2351 bp |
| Locus ID | 71701 |
| Cytogenetics | 11 A3.3 |
| MW | 85.7 kDa |
| Summary | RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"BC055826" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC220827 | Pnpt1 (untagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934), (10ug) | 10 ug |
$790.00
|
|
| MG210634 | Pnpt1 (tGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) | 10 ug |
$917.00
|
|
| MR210634L3 | Lenti ORF clone of Pnpt1 (Myc-DDK-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) | 10 ug |
$1,017.00
|
|
| MR210634L4 | Lenti ORF clone of Pnpt1 (mGFP-tagged) - Mouse polyribonucleotide nucleotidyltransferase 1 (cDNA clone MGC:67745 IMAGE:3498934) | 10 ug |
$1,017.00
|
|
Citations
Exploring the mitochondrial microRNA import pathway through Polynucleotide Phosphorylase (PNPase)
Shepherd, Hathaway, Pinti et al
J. Mol. Cell. Cardiol. (2017) 110, 15-25 DOI: 10.1016/j.yjmcc.2017.06.012
Shepherd, Hathaway, Pinti et al
J. Mol. Cell. Cardiol. (2017) 110, 15-25 DOI: 10.1016/j.yjmcc.2017.06.012
AI Summary: OriGene’s pre-miRNA-378 miRNA expression plasmid (pCMV-MIRNA, Product # sc401025) was used to generate HL-1 cardiomyocyte cell lines stably overexpressing miRNA-378, enabling the study of mitochondrial miRNA import and its effects on cellular bioenergetics in diabetes.
Snippet: 378 HL-1 overexpressing cell line (HL-1-378) generated by our laboratory was also used as previously described [50];stably overexpressing miRNA-378 through a pre-miRNA-378 miRNA expression plasmid (pCMV-MIRNA) (Product # sc401025;Origene, ... All 3 versions Cite SaveSaving
Showing 1-1 of 1 papers.
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