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Ift80 (NM_026641) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210591
Ift80 (Myc-DDK-tagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $711.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ift80
Synonyms 4921524P20Rik; mKIAA1374; Wdr56
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210591 representing NM_026641
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGACTGAAGATATCTCTCTCAAAGGAACCAAAGCATCAAGAATTAGTGAGCTGTGTGGGTTGGACTA
CGGCGGAGGAGCTGTACTCATGCAGTGACGACCACCAGATCGTCAAGTGGAACCTGCTAACCAGTGAAAC
CAGCCTAATAGTCAAGCTGCCTGATGACATCTACCCGATAGACCTTCACTGGTTTCCAAAGAGTTTAGGC
ATAAAGAAACAGACGCAGGCAGAAAGCTTCGTCCTCACAAGCTCTGATGGTAAGTTTCATCTGATTTCCA
AGTTAGGAAGAGTGGAAAAGAGTGTAGAAGCTCACTGTGGGGCTGTGCTCGCAGGACGATGGAATTATGA
GGGGACTGCATTAGTGACAGTTGGAGAAGATGGACAAGTGAAAATCTGGTCAAAGACAGGGATGCTCAGA
TCAACTTTAGCCCAGCAAGGAACACCAGTGTACTCAGTAGCCTGGGGCCCTGATTCAGAAAAGGTTCTTT
ATACAGCAGGCAAACAGCTCATCATTAAACCTCTTCAACCAAATGCTAAAGTTTTACAGTGGAAAGCCCA
TGATGGCATCATTTTAAAAGTCGACTGGAACTCAGTGAATGACCTTATTTTATCTGCGGGTGAAGACTGT
AAGTATAAGGTTTGGGATAGTTATGGCCGCGTGCTGTACGGCTCCCAGCCTCATGAGCACCCCATCACCT
CAGTGGCCTGGGCTCCAGATGGGGAGTTATTTGCCGTTGGATCGTTTCATACGTTACGCTTGTGTGATAA
AACTGGGTGGTCATATGCTTTAGAGAAACCCAACACTGGCAGCATATTTAACATTGCGTGGTCTATTGAT
GGCACTCAGATCGCGGGAGCCTGTGGGAACGGGCATGTTGTCTTTGCACATGTGGTGGAGCAGCGCTGGG
AGTGGAAAAATTTTCAAGTAACGCTAACGAAGAGAAGGACCATGCAGGTTCGCAACGTTCTCAATGATGC
CGTGGATCTCCTGGAGTTCCGGGACAGAGTCATTAAAGCATCCTTAAACCACGCACACTTGGTGGTCTCC
ACGTCCCTGCAGTGCTACGTGTTCTCTACGAAGAACTGGAATACACCACTTATATTTGATCTCAAAGAAG
GAACTGTTAGTTTAATTCTGCAGGCAGAAAGACATTTTCTTCTGGTGGATGGCGGGGGTATCTACCTGCA
CTCTTATGAAGGGCGCTTCATTTCTTCTCCAAAGTTTCCTGGCATGAGAACAGACATCCTGAACGCTCAG
ACTGTGTCTCTGAGCAATGACACGATAGCAATAAAGGACAAAGCTGACGAGAAGATAATCTTCCTCTTTG
AGGCGTCAACTGGAAAACCCCTAGGGGATGGGAAACTTCTTTCTCATAAGAATGAAATCTCAGAAATTGC
TCTGGATCAAAAAGGACTTACCAATGACAGAAAAATTGCTTTCATTGACAAGAACAGAGATCTCTACATC
ACCTCTGTGAAACGATTTGGGAAAGAAGAACAGATTATTAAACTTGGAACCATGGTACATACTTTGGCAT
GGTGTGATACATGCAATATCCTCTGTGGAATTCAAGATACTCGATTCACAGTATGGTATTACCCTAATAC
CATTTATGTGGACAGAGACATTCTGCCTAAAACATTGTACGAAAGAGATGCAAGTGAATATAGTAAAAAC
CCGCATATAGTGAGTTTTGTTGGAAATCAGGTAACTATAAGAAGAGCTGATGGCTCCCTGGTGCACATCA
GCATATCACCGTACCCCGCCATCCTCCATGAGTATGTGAGCAGCTCCAAGTGGGAAGAGGCTGTAAGGCT
TTGCCGCTTTGTTAAGGAGCAAAGCATGTGGGCTTGCCTAGCGGCTATGGCTGTTGCTAATCGAGATATG
GTCACTGCAGAAATAGCCTATGCAGCAGTTGGGGAAATTGATAAAGTTCGGTACATCAATGCTATAAAAG
ATCTTCCATCTAGAGAGTCAAAAATGGCCCACATACTCATGTTTAGTGGGAACATCCAGGAGGCTGAAAC
AGTCCTGCTCCAGGCTGGCCTTGTTTATCAAGCCATACAGATCAACATTAATCTCTATAACTGGGAAAGG
GCGCTGGAGTTGGCTGTAAAATACAAAACCCACGTTGATACAGTGCTCGCTTATCGTCAGAAGTTCTTGG
ACACATTTGGTAAACAGGAAACTAATAAACGATATTTACAATATGCAGAGGGCCTCCAAATAGACTGGGA
GAAAATCAAAGCCAAAATTGAGATGGAAATTACTAAAGAACGAGACCGATCATCCAGCGGCCAGTCCAGC
AAGAGTGTGGGCTTAAAGCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210591 representing NM_026641
Red=Cloning site Green=Tags(s)

MRLKISLSKEPKHQELVSCVGWTTAEELYSCSDDHQIVKWNLLTSETSLIVKLPDDIYPIDLHWFPKSLG
IKKQTQAESFVLTSSDGKFHLISKLGRVEKSVEAHCGAVLAGRWNYEGTALVTVGEDGQVKIWSKTGMLR
STLAQQGTPVYSVAWGPDSEKVLYTAGKQLIIKPLQPNAKVLQWKAHDGIILKVDWNSVNDLILSAGEDC
KYKVWDSYGRVLYGSQPHEHPITSVAWAPDGELFAVGSFHTLRLCDKTGWSYALEKPNTGSIFNIAWSID
GTQIAGACGNGHVVFAHVVEQRWEWKNFQVTLTKRRTMQVRNVLNDAVDLLEFRDRVIKASLNHAHLVVS
TSLQCYVFSTKNWNTPLIFDLKEGTVSLILQAERHFLLVDGGGIYLHSYEGRFISSPKFPGMRTDILNAQ
TVSLSNDTIAIKDKADEKIIFLFEASTGKPLGDGKLLSHKNEISEIALDQKGLTNDRKIAFIDKNRDLYI
TSVKRFGKEEQIIKLGTMVHTLAWCDTCNILCGIQDTRFTVWYYPNTIYVDRDILPKTLYERDASEYSKN
PHIVSFVGNQVTIRRADGSLVHISISPYPAILHEYVSSSKWEEAVRLCRFVKEQSMWACLAAMAVANRDM
VTAEIAYAAVGEIDKVRYINAIKDLPSRESKMAHILMFSGNIQEAETVLLQAGLVYQAIQININLYNWER
ALELAVKYKTHVDTVLAYRQKFLDTFGKQETNKRYLQYAEGLQIDWEKIKAKIEMEITKERDRSSSGQSS
KSVGLKH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026641
ORF Size 2331 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026641.2, NP_080917.1
RefSeq Size 4090 bp
RefSeq ORF 2334 bp
Locus ID 68259
UniProt ID Q8K057
Cytogenetics 3 E1
MW 87.8 kDa
Summary Component of the intraflagellar transport (IFT) complex B, which is essential for the development and maintenance of motile and sensory cilia.UniProtKB/Swiss-Prot Function
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Documents
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"NM_026641" in other vectors (4)
SKU Description Size Price
MC221499 Ift80 (untagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80), (10ug) 10 ug
$784.00
MG210591 Ift80 (tGFP-tagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80) 10 ug
$911.00
MR210591L3 Lenti ORF clone of Ift80 (Myc-DDK-tagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80) 10 ug
$1,011.00
MR210591L4 Lenti ORF clone of Ift80 (mGFP-tagged) - Mouse intraflagellar transport 80 homolog (Chlamydomonas) (Ift80) 10 ug
$1,011.00

Plasmid Map for MR210591

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210591
Circular map for MR210591

file-alt Citations

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