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Sema3a (NM_009152) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR210565
Sema3a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a)
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Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $707.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sema3a
Synonyms coll-1; Hsema-I; SEMA1; Semad; SemD
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR210565 representing NM_009152
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTGGTTCACTGGGATTGCCTGTCTTTTCTGGGGTGTATTACTTACAGCCAGAGCAAACTATGCAA
ACGGAAAGAACAATGTGCCAAGACTGAAATTATCGTACAAAGAAATGTTGGAATCCAACAATGTGATCAC
TTTTAATGGCTTGGCCAACAGCTCCAGTTACCACACCTTCCTTCTGGATGAAGAACGGAGTAGACTATAT
GTTGGAGCAAAAGATCATATATTTTCATTCAACTTGGTGAACATTAAAGATTTTCAAAAGATTGTGTGGC
CAGTATCTTACACAAGGAGAGATGAATGCAAATGGGCTGGAAAAGATATCCTGAAAGAATGTGCCAATTT
CATCAAGGTCCTGGAGGCTTATAATCAGACTCACTTGTATGCCTGTGGAACTGGGGCTTTCCATCCAATC
TGCACCTATATTGAAGTTGGACATCATCCTGAGGACAACATTTTTAAGCTGCAGGACTCACATTTTGAAA
ACGGTCGTGGGAAGAGCCCTTATGATCCCAAACTACTGACTGCCTCTCTTCTAATAGACGGTGAGTTGTA
CTCTGGAACTGCTGCGGACTTCATGGGACGGGACTTCGCTATCTTCAGAACACTGGGGCACCATCACCCC
ATCAGGACGGAGCAGCATGACTCCCGGTGGCTCAATGATCCTAGATTCATCAGTGCCCATCTCATCCCAG
AGAGTGACAACCCTGAAGATGACAAAGTATATTTTTTCTTCCGAGAAAATGCAATAGACGGAGAACACTC
TGGAAAAGCCACTCATGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGTGGACACAGAAGTCTTGTG
AATAAATGGACAACATTCCTAAAAGCACGCCTGATTTGCTCTGTGCCCGGTCCCAATGGCATTGACACCC
ATTTTGATGAATTGCAGGATGTATTCCTAATGAACTCTAAAGATCCTAAAAATCCGATCGTCTATGGAGT
GTTCACAACATCAAGCAACATCTTTAAGGGATCTGCTGTGTGCATGTACAGCATGAGTGATGTAAGAAGG
GTGTTCCTTGGTCCATATGCTCACAGAGATGGTCCCAACTATCAGTGGGTGCCTTACCAAGGAAGAGTCC
CTTATCCACGGCCAGGAACTTGTCCCAGTAAAACATTTGGCGGATTTGACTCCACAAAGGACCTTCCTGA
TGATGTCATAACTTTTGCAAGAAGTCATCCAGCCATGTACAACCCAGTGTTTCCTATAAATAATCGCCCG
ATCATGATCAAAACAGATGTAAATTATCAGTTCACACAAATTGTTGTAGACCGAGTGGATGCAGAAGATG
GCCAGTATGATGTTATGTTCATCGGAACAGATGTTGGAACCGTTCTTAAAGTGGTTTCAGTCCCCAAGGA
GACTTGGCATGACCTAGAAGAAGTTCTTCTGGAAGAAATGACCGTCTTCCGGGAACCAACAACTATTTCG
GCAATGGAGCTTTCTACTAAACAGCAACAGCTGTACATTGGCTCAACTGCGGGAGTGGCACAGCTTCCTC
TACACCGCTGTGACATCTATGGCAAAGCCTGTGCAGAATGCTGCCTCGCTCGGGACCCTTACTGTGCCTG
GGATGGGTCCTCATGCTCACGCTATTTTCCTACTGCAAAGAGGCGCACAAGACGACAAGATATAAGGAAT
GGAGACCCACTGACTCACTGCTCTGACTTGCAGCACCATGATAATCATCATGGGCCCAGCCTTGAAGAGA
GAATCATCTATGGAGTGGAAAACAGTAGTACATTCTTGGAATGCAGTCCGAAGTCACAGAGAGCCTTGGT
ATATTGGCAATTTCAGAGGAGAAATGAAGATCGAAAAGAGGAGATCAGAATGGGTGATCATATCATCAGG
ACAGAACAAGGGCTCCTGCTCCGTAGCCTGCAGAAGAAGGATTCAGGCAATTACCTGTGTCACGCTGTGG
AACACGGATTCATGCAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAACATTTGGAAGAACT
TCTTCATAAAGATGACGATGGAGATGGCTCTAAGATAAAAGAAATGTCGAGCAGCATGACGCCCAGCCAG
AAAGTCTGGTACCGAGACTTCATGCAGCTCATTAACCACCCCAACCTGAACACGATGGATGAGTTCTGTG
AACAAGTGTGGAAAAGGGACCGAAAGCAACGCCGACAAAGGCCGGGGCACTCTCAAGGGAGCAGCAACAA
GTGGAAGCACATGCAAGAGAGCAAGAAAGGTAGAAACAGGAGGACCCACGAGTTTGAGCGGGCACCCAGA
AGTGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR210565 representing NM_009152
Red=Cloning site Green=Tags(s)

MGWFTGIACLFWGVLLTARANYANGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY
VGAKDHIFSFNLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLEAYNQTHLYACGTGAFHPI
CTYIEVGHHPEDNIFKLQDSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP
IRTEQHDSRWLNDPRFISAHLIPESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV
NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNSKDPKNPIVYGVFTTSSNIFKGSAVCMYSMSDVRR
VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPINNRP
IMIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSVPKETWHDLEEVLLEEMTVFREPTTIS
AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSSCSRYFPTAKRRTRRQDIRN
GDPLTHCSDLQHHDNHHGPSLEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEDRKEEIRMGDHIIR
TEQGLLLRSLQKKDSGNYLCHAVEHGFMQTLLKVTLEVIDTEHLEELLHKDDDGDGSKIKEMSSSMTPSQ
KVWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHSQGSSNKWKHMQESKKGRNRRTHEFERAPR
SV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009152
ORF Size 2316 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009152.4
RefSeq Size 5946 bp
RefSeq ORF 2319 bp
Locus ID 20346
UniProt ID O08665
Cytogenetics 5 A1
MW 89.2 kDa
Summary Plays a role in growth cones guidance. May function to pattern sensory projections by selectively repelling axons that normally terminate dorsally. Involved in the development of the olfactory system and in neuronal control of puberty (By similarity).UniProtKB/Swiss-Prot Function
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"NM_009152" in other vectors (3)
SKU Description Size Price
MC205153 Sema3a (untagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a), (10ug) 10 ug
$708.00
MR210565L3 Lenti ORF clone of Sema3a (Myc-DDK-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a) 10 ug
$1,007.00
MR210565L4 Lenti ORF clone of Sema3a (mGFP-tagged) - Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (Sema3a) 10 ug
$1,007.00

Plasmid Map for MR210565

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR210565
Circular map for MR210565

file-alt Citations

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